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The giant panda-rabbit interspecies reconstructed embryos were constructed by injection of giant panda somatic cells into the perivitelline space of the enucleated oocytes and fused by electrical stimulation, and the reconstructed embryos can develop to hatched blastocyst stage in vitro. We demonstrated that the nuclear genetic materials in reconstructed blastocyst cells were the same as that of giant panda somatic cells by chromosome count and the existence of characteristic chromosome, which was confirmed by microsatellite DNA analysis later In order to determine the fate of mtDNA of giant panda-rabbit reconstructed embryos, we analyzed the cyt b sequence of embryos at 1 -cell stage, 2-cell stage, 4-cell stage, 8 cell stage, morula stage, blastocyst stage, expanded blastocyst stage and hatched blastocyst stage.

异种克隆大熊猫重构胚是通过将大熊猫成纤维细胞移入日本大耳白兔去核卵母细胞透明带下后经电融合构建的,可在体外发育至孵化囊胚阶段,我们应用一种简便、快速的异种重构胚的鉴定方法,即通过染色体数目和特征染色体的存在初步鉴定出大熊猫-兔异种重构胚的核遗传物质来源于大熊猫供核体细胞(后经昆明动物研究所通过微卫星DNA分析验证了该结果的可靠性)。

Sp. tritici conidiospores. 40 h after inoculation, penetration of the fungus and formation of haustoria in transformed cells were observed to evaluate the effect of the target gene's product on the invasion of the fungus.

基因枪轰击后高密度接种白粉病菌孢子。40h后观察转化阳性表皮细胞度其表面抱子的发育,并以阳性转化细胞中白粉病菌成功侵入的细胞所占比例为指标分析目标基因的表达对白粉病菌入侵及吸器形成产生的影响(对照为单独导入GUS基因的表皮细胞)。

RPC steel and RQ steel are constituted by bainite and martensite microstructures before tempering. The microstructure of RPC steel did not change obviously except some bainitic laths coalescing were detected by means of SEM during tempering, while the boundary of bainitic laths of RQ steel disappeared quickly and finally formed polygonal ferrite forms accompanied a dramatic decrease in hardness.

回火前两种钢板组织均为贝氏体和马氏体的复合组织,经RPC处理后的钢板随回火温度的提高组织没有明显变化,只是一些板条出现合并现象,而经过RQ处理后的钢板随回火温度的升高板条很快消失最终演变成多边形铁素体,硬度也随之大幅度下降。

Two series of polyurethane acrylate grafted acrylic resins (Acr-g-TPU-PETA and Acr-gPPU-PETA) self-emulsifying systems are obtained by grafting -NCO group modified polyurethane acrylates with acrylic copolymer prepared from n-butyl acrylate, styrene,βhydroxyethyl methacrylate and acrylic acid The self-emulsifying systems showed Newtonian fluidity with viscosity closed to that of water, the solid contents were around 30% The T〓 of 15~4℃ for dried film before photocuring favored film formation at room temperature DSC and DMA results showed that PU-PETA is compatible with acrylic resin in the grafted resin The TG and dTG curves for Acr-g-PU-PETA samples were substantially different from the corresponding blends, and the brodened dTG peaks for photocured grafted resin suggest that there might be multiple interactions among chain segments The photocued film of the grafted resins self-emulsifying system exhibited good overall properties with polyurethane acrylate grafts ranging from 30% to 50%, and Acr-g-TPU-PETA series showed better properties than Acr-gPPU-PETA series The adhesion of the photocured film was 1~2 grade, impact strength was 50kg-cm and flexibility was 1mm The Shore hardness and the adhesion of the film decreased markedly if PETA was replaced by HEMA as photoactive group due to the lower C=C double bond content in the grafted resin The polyhydroxy amine-linked thioxanthones photoinitiators can be evenly dispersed in self-emulsifying system without color spot appearing in the dried film The films photocured with IV〓~IV〓 generally exhibited higher Shore hardness, gloss and solventresistance than that cured with Irgacure 2959 and did not induce the rusting of the iron substrate Pigmenting with titanium oxide had no effect on the photocuring The polyhydroxy amine-linked thioxanthone IV〓 and IV〓 can be new candidates for the formulation of titanium oxide pigmented photocurable water-borne coatings for their pale-yellow appearance and slight interference with the color of the coatings WB 4759 with high water-solubility coalesced and separated from the dried resin film, and even induced the rusting of iron substrate.

以丙烯酸丁酯,甲基丙烯酸-β-羟乙酯,丙烯酸及苯乙烯合成厂丙烯酸共聚树脂,并与含-NCO端基的聚氨酯丙烯酸酯接枝反应,经水性化后,得到两个系列(Acr-g-TPU-PETA和Acr-g-PPU-PETA)各5个不同配比的丙烯酸树脂接枝聚氨酯丙烯酸酯自乳化体系,粘度与水相近,为牛顿流体,固含量30%左右。光固化前干燥涂膜的玻璃化转变温度在-15℃~4℃之间,有利于室温下成膜。DSC及DMA谱表明两种树脂基本相容。Acr-g-PU-PETA的热失重行为明显不同于共混材料,接枝样dTG峰明显宽化,表明接枝样中链段之间的相互作刚有多重性,丙烯酸树脂与聚氨酯之间有一定的相容性。接枝树脂自乳化体系光固化涂层的综合性能以聚氨酯丙烯酸酯成分为50%~30%者相对较好,且以Acr-g-TPU-PETA系列更理想。光固化膜的附着力为1~2级,冲击强度为50kg·cm,柔软性多为1mm。以HEMA代替PETA作为接枝树脂的活性基团,碳—碳双键密度太低,光固化膜交联点太少,硬度和附着力均较差。多羟基胺连硫杂蒽酮光引发剂能均匀分散于树脂自乳化体系和接枝树脂干膜中,不出现色斑,光固化膜肖氏硬度一般高于以Irgacure 2959为光引发剂的光固化膜,光泽度及耐丁酮溶剂性能均有所提高,不会诱导铁基生锈,钛白着色对光固化结果几乎没有影响。本论文所合成的多羟基胺连硫杂蒽酮光引发剂IV〓与IV〓为浅黄色,对光固化涂层的颜色干扰较小,为配制钛白着色光固化水性涂料提供了新的光引发剂。水溶性太强的WB 4759在干膜中易聚结析出,且对所附着的铁基有诱导锈蚀作用。

So the defocus quantity is calculated by detecting the image spot position offset and the front focus plane of the collimation lens can be adjusted on the first image of the theodolite.

该方法利用半反半透镜将准直物镜后的物光折转,采用双分离透镜将目标成像到CCD上,经视频驱动、放大电路、再经A/D转换后输入到微机进行图像信号处理,这样,通过检测像的偏移量求出离焦量的大小和方向,然后驱动调焦部件使准直镜的前焦点在经纬仪的第一像面上。

In order to obtain the correct height reconstruction of a measured object through the traditional Fourier transform profilometry, the connecting-line between the exit pupil of the projecting lens and the entrance pupil of the imaging lens must be horizontal and set to parallel to the reference plane.

在拍摄过程中,采集完参考平面的光场后,只需要再采集一帧条纹图形,通过傅里叶变换、频谱滤波、逆傅里叶变换、相位展开等处理后就能得到物体的三维数据。采用传统的傅里叶变换轮廓术进行三维物体面型测量时,必需保证投影装置出射光瞳和成像装置入射光瞳的连线与参考面平行,并且它们的光轴应在同一平面上,才能得到较为准确的测量结果。

Then based on CWMS, we propose a new method to enhance the resolution of seismic traces. Firstly, we divide the seismic signal into proper segments by Gabor molecule-windows adaptive to the signal, where each segment is regarded stationary, and then transform it into the Gabor domain. Secondly, we deal with the amplitude spectrum for each segment to expand the frequency band of the spectrum in the Gabor domain. Finally, taking inverse Gabor transform on modified time-frequency coefficients, we get the high-resolution signal.

然后基于变子波模型,提出了一种新的提高地震记录分辨率的方法:第一步,用自适应于地震记录的Gabor分子窗把地震记录恰当地划分成若干片断,每段内信号近似平稳,然后将地震记录变换到时间-频率域;第二步,在变换域对每个分子窗内信号的振幅谱进行处理以拓宽频带;最后把处理后的时间-频率域函数反变换回时间域得到提高分辨率后的结果。

When I had consider'd this a little, it follow'd necessarily, that I was certainly in the Wrong in it, that these People were not Murtherers in the Sense that I had before condemn'd them, in my Thoughts; any more than those Christians were Murtherers, who often put to Death the Prisoners taken in Battle; or more frequently, upon many Occasions, put whole Troops of Men to the Sword, without giving Quarter, though they threw down their Arms and submitted.

于是,我对自己的计划也改变了看法,并开始冷静地考虑我自己的行动。我想:这么多世纪以来,上天都容许这些人不断互相残杀而不惩罚他们,那我有什么权力和责任擅自将他们判罪处死,代替上天执行对他们的判决呢?这些人对我又究竟犯了什么滔天大罪呢?我又有什么权力参与他们的自相残杀呢?我经常同自己进行辩论:&我怎么知道上帝对于这件公案是怎样判断的呢?毫无疑问,这些人并不知道他们互相吞食是犯罪行为;他们那样做并不违反他们的良心,因而他们也不会受到良心的谴责。他们并不知道食人是违背天理的罪行而故意去犯罪,就像我们大多数人犯罪时一样。他们并不认为杀死战俘是犯罪行为,正如我们并不认为杀牛是犯罪行为;他们也不认为吃人肉是犯罪行为,正如我们并不认为吃羊肉是犯罪行为。&我稍稍从这方面考虑了一下,就觉得自己不对了。我感到他们并不是我过去心目中所谴责的杀人犯。有些基督徒在战斗中常常把战俘处死,甚至在敌人已经丢下武器投降后,还把成队成队的敌人毫无人道地杀个精光。从这方面来看,那些土人与战斗中残杀俘虏的基督徒岂不一样!

The power of motor is positive proportion to square root of equality of square pure torque .the pure torque is combined by suspension point torque and balancing weight torque ,so the change of suspension point's motion law and balancing weight offset angle can causes pure torque variation .especially, the suspension motion law is decided by configuration curve of hinder heaven sheave .so the paper has emphatically studied and analysis the law that the configuration curve of hinder heaven sheave brings the change of pumping unit energy-saving .

电机功率与减速器曲柄轴净扭矩均方根成正比,减速器曲柄轴净扭矩是由悬点载荷产生的扭矩和平衡重产生的平衡力矩合成的;改变悬点运动规律和平衡重的滞后角可以引起净扭矩的变化,其中悬点运动规律是由后天轮曲线决定的,因此,本文依据此原理着重研究分析了后天轮曲线影响抽油及节能的变化规律。

This paper presents conductive polymer molecular materials with insulating powder, mixed into the deployment of surge suppressors for low capacitance of paste, while the component design concept is based on the field of passive components, manufacturing process to make the basic theory to design the protective components, the main use of the existing ceramic substrate for the component body structure design, screen design in the body through the structure of the gap at the top of the printing layer of a different conductors, and then to learning to know the printing process technologies approach the low capacitance of the surge suppressor paste cover the gap in the two conductors, and by the resistance of plastic burning process technology, the temperature of sintering parts made of electrostatic protection element, and this protection through the external ESD components in bombardment tests, measurement of capacitance electrostatic discharge after bombardment, Trigger voltage and leakage current data, and the volume measured by the results of future discussion of the layout of this different style of work in the same area, different clearance, solid content and temperature than the next, for the attainment of performance components, a low capacitance ESD protection devices with low breakdown voltage requirements and to chip-based protection devices can be designed to integrate with the advanced Integrated Circuits or used independent of the electrostatic protection element in the system.

本文提出高分子导电分子材料搭配绝缘粉末,调配混合成适用於低电容突波抑制器之PASTE,而元件设计概念是以被动元件制程领域作基础理论去设计此防护元件,主体利用现有陶瓷基板为元件本体结构设计,在经由网版设计在本体结构上方印刷一层不同的间隙导体,再以习知印刷制程技术方式,将低电容突波抑制器之PASTE覆盖在两导体的间隙内,并经由电阻制程习知塑烧技术,将元件烧结适当温度制成静电防护元件;而此防护元件在经外加静电放电轰击测试后,量测静电放电轰击后的电容、Trigger voltage及漏电流相关数据,并藉由测结果讨此同的布局样式,在相同工作区面积、不同间隙、固含量与温度比下,对於元件效能是否达到静电防护元件低电容与低崩溃电压的需求,并藉此晶片型防护元件可设计整合於先进积体电或应用於独系统的静电防护元件中。

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Shop assistant: Please check at the door.

店员:请您到门口柜台交费。

In the end, transportation of PAHs in the environmental matrix was preliminarily investigated and discussed according to PAHs data of soils and sediments from a certain region in Shanghai. The result indicated that there are two or more pathways on PAHs transportation in the environmental matrix.

最后,对上海特定地区内PAHs在环境介质中的迁移径途进行了分析讨论,结果表明上海市土壤和沉积物等环境介质中PAHs的迁移存在多种途径。

Quick stop! AIDS doctors while the side and say hello and colleagues mention a blood sample box, the Red Cross had sent blankets and so on, with patients有说有笑to go home.

快停车!防艾医生们一边招呼一边和和同事们提着血液采样箱,抱着红十字会送来的被子等,有说有笑地同患者往家走。