后成的

After 20 d calcium induction, jacinth calcium salts could be detected in the cells by alizarin red staining.At day 10 after being adipoinduced, the cells showed positive oil red O staining.

结果：成功获得了稳定表达GFP的ADSCs，GFPADSC经成骨诱导20 d后茜素红染色可见有橘红色钙盐沉积；经成脂诱导10 d后油红O染色阳性。

After 20 d calcium induction, jacinth calcium salts could be detected in the cells by alizarin red staining.At day 10 after being adipo induced, the cells showed positive oil red O staining.

结果：成功获得了稳定表达GFP的ADSCs，GFP ADSC经成骨诱导20 d后茜素红染色可见有橘红色钙盐沉积；经成脂诱导10 d后油红O染色阳性。

The adipoinduced MSCs were detected with oil red O staining. RESULTS: ADSCs, stably expressing GFP, were obtained successfully from the transgenic mice with GFP gene. After 20 d calcium induction, jacinth calcium salts could be detected in the cells by alizarin red staining.

结果：成功获得了稳定表达GFP的ADSCs，GFPADSC经成骨诱导20 d后茜素红染色可见有橘红色钙盐沉积；经成脂诱导10 d后油红O染色阳性。

In the scope of our life made cards and membership cards are made of a circle or rounded corners or other irregular situation, this is when the business card printing and membership card up forming post-pressure sected, in which the effect of cutting way is to become a wooden model and then separated from the surrounding steel blade edge around it, and then be immediately arotic post-pressure sected.

在吾人生活范围里制卡和会员卡制作物经常有圆形或圆角或其他不规则情形，这是当制卡和会员卡制作完毕后压切成型的效果，此种压切方式是先装成一个木制模型然后由周围以薄型钢片刀顺其边缘围绕，再加以紧搏后压切。

Results一. Culturing and identification of human conjunctiva cells1. morphous of conjunctiva cellsCells were harvested by tissue cultivation after digestion. Ponderosus cells adhered after 48 hours. Cells were shown in shape of round, ellipse and polygon. Cell body was loose and lucency with nucleus in center, cell membrane was also clearly seen. Cells were arranged in inlay and fuse in film after 12-14 days.2. Immunochemistry testNomogeneous immunochernical positive granules against CK13 were observed in endochylema of human conjunctiva cells.3. growth curve of conjunctival cellsSubcultured cells were aged and feeble after 5th passage.

结果一、正常人结膜细胞的培养与鉴定1、活体细胞的形态消化后组织块培养法：48小时后见大量上皮细胞贴壁，细胞不规则呈圆形、椭圆形、多边形，细胞体肥大透亮，细胞核位于中央，胞膜清楚，约12-14天后细胞呈镶嵌状排列，融合成膜状。2、免疫组化鉴定培养的人结膜上皮细胞CK13免疫组化染色见阳性颗粒均匀分布于胞浆中。3、培养结膜细胞生长曲线传代培养多数样本于P5代以后开始出现衰老征象。

Methods Linkmax HV, Nexus 2(NX2), Variolink Ⅱ HV asdual-cured resin luting agents were bonded to silanized GN-Ⅰ glass ceramics, and irradiated by 800, 310 and 80 mW.cm-2light intensity to form micro-shear test specimens. After 1, 90 d water storage, micro-shear bond strength of silanized resin/ceramic luting agent were measured.

选取Linkmax HV、Nexus 2(NX2)、Variolink Ⅱ HV3种双重固化树脂粘接剂，使之与硅烷偶联剂处理的玻璃陶瓷粘接，接受800、310、80 mW·cm-2光强度照射后，制作成微剪切试件，然后在水储存1、90 d后，接受剪切实验。

And after the death of God, man became God, and created a post-industrial reality equipped with high technology.

上帝死后，人成了上帝，创造了高技术化的后工业现实。

METHODS: The rib trabeculae were resected and broken, trypsinizated and washed completely by PBS. Bone surface and non-adhesive floating cells in cleaning fluid were observed with inverted microscope. Rib trabeculae was washed by DMEM culture medium once, and cultured in culture bottle. The culture liquid was replaced by new one once a week. The osteoblast was moved from the sclerite a week later. The cells were fused monolayer and could be subcultured 4 to 6 weeks later.

切下人肋骨骨小梁剪碎，胰酶消化后以PBS彻底冲洗直至于倒置显微镜下观察骨片表面及清洗液中无粘着及漂浮的细胞，以DMEM完全培养液清洗骨小梁1次，移入培养瓶内培养，每周更换培养液1次，1周后可见成骨细胞自骨片移出，4~6周细胞融合成单层可作传代培养。

When dissolved gases and solids mix with pure water, the result is sea water, or salt water.

当分解后的气体和固体和纯水混合后，水就成了海水或盐水。

RESULTS: Persistent positive expression of vascular endothelial growth factor was seen in the epidermal cells and sebaceous gland cells.

阳性细胞数量随损伤时间的延长而增多，于伤后96 h达峰值，伤后1周和2周仍见少量巨噬细胞和成纤维细胞表达阳性。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。