后成的

Methods:Human CD80 cDNA was transfected into B16 melanoma cells by lipofectin-mediated gene transfer before the expansion of tumor cells were tested by MTT method in vitro;C57BL/6 mice were inculated subcutaneously either mock-transfected B16 cells (B16-neo)or CD80-transfected B16 cells (B16-CD80),then the latency,survival times and tumor mass growth were investigated.The lymphocytes were examined for both proliferation indices and specific cytotoxic activity by MTT method and improved LDH-releasing method,after syngenic Mixed Lymphocyte tumor cultures.

通过脂质体介导将CD80基因导入小鼠黑色素瘤B16细胞后，利用SABC法检测CD80的表达；MTT法测定肿瘤细胞体外增殖能力；将肿瘤细胞接种至同源小鼠皮下后观察成瘤期、荷瘤小鼠存活期及肿瘤结节生长速度；在同源淋巴细胞肿瘤细胞混合培养后，通过测定淋巴细胞增殖指数和CTL杀伤活性检测肿瘤细胞的免疫原性。

Accor- to early or late hydrocarbon generation and whether the original sealing systems have been broken in the course of primary hydrocarbon accumulation through to the finalization of the oil and gas pools,the reservoiring models of petroleum in marine strata in southern China can be divided into three types: including primary,se- and regeneration types.

依据生烃期的早晚以及油气在完成初次聚集后至最终成藏定型是否突破了原始封闭体系，南方海相油气成藏模式可划分为原生型、次生型和再生型3种类型。

Most of the Mesozoic and Cenozoic rifted basins in East China experienced magmation to some extent during or after the basin formation.

中国东部中、新生代裂陷盆地在成盆期或成盆期后大多都发生过不同程度的岩浆活动。

This went on for about twelve years, during which I sermonized on Socialism at least three times a fortnight average.

这种情况大约持续了十二年。在此期间，我宣传社会主义，平均两个星期至少要讲三次。（后半句单独成句，其中年的时间状语可以译成并列谓语。

In order to cater the generalization of shuttleles looms and hi-efficient production and upgrading the general quality of the knitting yarn,analysis is done to the impact of the snicks of the knitting yarn and the special structure of the knitting fabric before highlight is given to the main cause leading to snicks of resultant yarn and to the technological counter-measures against the snicks.

为了适应无梭织机普及和高效率生产的需求，提高针织纱整体质量，在分析针织纱成纱细节疵点危害性及针织物独特组织结构要求的基础上，重点阐述了成纱细节疵点产生和增加的主要原因及降低成纱细节应采取的相关技术措施，指出通过采用合理的配棉，优选前、后纺工艺参数，保持良好的设备状况，合理选用新型优质的纺纱专件、器材，强化日常性的操作与清整洁工作，重视各工序的温湿度管理，可明显减少针织纱的细节疵点，提高成纱质量的整体水平。

The warp and weft of this product all adopts high-quality PA6 raw materials, and through bright-drawing,cooling and so on procedures form into transparent yarn with .The warp yarn is made into warp spindle by an intelligent beaming machine,woven into blank screen on the flexibility rapier loom directly.

该产品经纬均采用优质PA6原料，经拉光、冷却等多道工序形成表面光滑、防水、防油、无毒、凉爽感的透明纱线，经纱通过智能型整经机制成经轴，直接在挠性剑杆织机上织造坯网，再经染色定型等后整理成最终产品。

When Ma Lan card upgrade will increase 7 percent of the offense and defense, blood an increase of 5 million upgrade to increase 9% in the offensive and defensive yellow, 80,000 blood, green horse upgrade license plus 11% attack and defense, 110,000 blood, purple increase offense and defense 13%, 1000 140,005 blood, and 2% will increase the value of life.

当马牌升级成蓝后，将加7%的攻防，血增加5万，升级成黄色加9%攻防，8万血，升级成绿色马牌加11%攻和防，11万血，紫色增加攻防13%，14万5千的血，而且都会加2%的生命值。

According to the construction course and finished dead state of the inclined tower cable-stayed bridge without back stay at Tongling Road in Hefei and combining the modern theory and method of the bridge construction control mentioned in this paper, established a space model by large-scale general finite element software ANSYS. It can obtain some valuable result by emulating analysis of the bridge which is under the construction course and finished dead state. It can provide an instruction for the Tongling Road bridge with the result and construction job site monitoring data.

文中根据合肥市铜陵路预应力混凝土无背索斜塔斜拉桥的具体施工过程和成桥状态，结合文中介绍的现代桥梁施工控制理论和方法，运用大型通用有限元软件ANSYS建立了三维空间模型，对该桥进行了施工过程和成桥状态下的结构仿真模拟分析，将模拟计算结果与施工现场振动频率法索力测试数据和分析结果相结合，为铜陵路无背索斜塔斜拉桥拉索张拉施工提供指导，及时调控，确保了该桥在成桥后线形美观，受力性能满足要求。

Methods 20 patients with complete left bundle branch block and 20 normal subjects were studied.

方法对20例完全性左束支传导阻滞患者和20例正常人解剖M型在心室波群区观察室间隔与左室后壁运动；运用频谱多普勒成像技术测量左、右心室射血前期时间（LVPET、RVPET），计算两者之差PETd；采用定量组织速度成像技术测量右室侧壁基底段和左室各壁基底段达收缩期峰值速度时限和达舒张早期峰值速度的时限。

A total of 391 bullfrogs were measured during the study period, including 113 adults and 278 juveniles.

共采集到变态后的牛蛙391只，包括牛蛙成体113只和亚成体278只。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。