后成的

4DRG was co-culture with sciatic nerve segment in 10鸖 DMEM;the axons were longer and surround the sciatic nerve segment which was regard as anew evidence for chemotropism.

结果：(1)在无血清条件下单独培养的DRG，背根神经节的轴突数目众多，外形纤细弯曲，不成束，并且施万细胞和成纤维细胞稀少，所以可以排除两者对轴突生长的影响，为观察来源于变性坐骨神经段的可溶性因子对轴突生长的作用提供了有利条件；(2)在无血清条件下DRG和变性坐骨神经段联合培养，①先单独培养DRG，4天后待神经元轴突长出，再与坐骨神经段联合培养，观察到神经元的轴突数目减少，外形挺直，部分轴突之间相互粘附成束；②变性坐骨神经段和DRG同时联合培养，神经元的轴突数目明显减少，外形粗壮，轴突之间相互粘附成束；(3)有血清条件下单独培养DRG，轴突数目较多，外形挺直，长短不一，部分神经元的轴突之间相互粘附，施万细胞和成纤维细胞数目众多，观察到的轴突生长情况受到施万细胞和成纤维细胞的直接或者间接的影响。

Results Compared to those before treatment the volume of the implanted keloid of Group D began to decrease since 14 days after treatment time-dependently (all P 《 0.05), and the volumes of the other 3 groups continued to increase and peaked on days 21, 14, or 7 respectively (all P 《 0.05).Microscopy showed infiltration of a larger quantity of histiocyte in the keloid tissue, and more obvious collagen disorganization and apoptosis of fibroblasts in Group D than in the other 3 groups.The protein expression of Bcl-2 was more remarkable and the protein expression of BAX was less remarkable in Group D than iu the other 3 groups.

结果 用药前和用药后7、14、21、28、35 d，D组瘢痕疙瘩组织块体积(mm3)分别为173±5、172±5、147±5、125±6、112±7和84±9，从用药后14 d开始明显缩小(均P＜0.05)；而其他3组瘢痕疙瘩组织块体积均明显增大，从用约后7 d开始各时点测得的体积均明显大于D组(均P＜0.05)。D组瘢痕疙瘩组织中有大量小鼠组织细胞浸润，胶原结构破坏和成纤维细胞凋亡明显重于其他3组，Bcl-2蛋门质表达明显弱于而Bax蛋白质表达明显强于其他3组。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

To those friends possessing computer,by super herosoft2000,the selected obbligato music can be compressed into MP3 or transfered to WAV form,then to make a obbligato CD.

有计算机的朋友可以利用超级解霸2000将选定的伴奏音乐压缩成MP3 或转成WAV 格式文件后再刻录成光盘。

Shim current calculation When shim coils are excited by appropriate current, they can generate correcting magnetic field to compensate the imaging magnetic field unhomogeneity. Based on the indirect calculation method, we proposed a novel iterative optimization algorithm to calculate shim current. Because there is a direct proportion relationship between FID signal and magnetic field homogeneity, we use the intensity of FID signal as feedback information to the calculation. Shim current can be worked out in less than 3 minutes. This satisfied the requirement that active shimming should be performed in real time.

5匀场电流计算对匀场线圈施加适当的电流后才能产生校正磁场消除成像磁场的非均匀分量，本文在匀场电流间接计算方法的基础上，利用磁共振成像原理中磁场均匀性与系统FID信号强度成正比的关系提出了一种电流迭代优化算法，将系统FID信号强度的变化作为反馈信息，能够快速计算出匀场电流对成像磁场进行补偿校正，平均耗时不到三分钟，满足磁共振成像系统对有源匀场的实时性要求。

Shim current calculationWhen shim coils are excited by appropriate current, they can generate correcting magnetic field to compensate the imaging magnetic field unhomogeneity. Based on the indirect calculation method, we proposed a novel iterative optimization algorithm to calculate shim current. Because there is a direct proportion relationship between FID signal and magnetic field homogeneity, we use the intensity of FID signal as feedback information to the calculation. Shim current can be worked out in less than 3 minutes. This satisfied the requirement that active shimming should be performed in real time.

5匀场电流计算对匀场线圈施加适当的电流后才能产生校正磁场消除成像磁场的非均匀分量，本文在匀场电流间接计算方法的基础上，利用磁共振成像原理中磁场均匀性与系统FID信号强度成正比的关系提出了一种电流迭代优化算法，将系统FID信号强度的变化作为反馈信息，能够快速计算出匀场电流对成像磁场进行补偿校正，平均耗时不到三分钟，满足磁共振成像系统对有源匀场的实时性要求。

With the increase of cell density, soma was slenderer and slenderer and like fibroblast.② Growth curves of PDMCs: Growth latency ranged from 2 to 8 days after cell inoculation. During this period, adherence was observed gradually but not obviously amplified. Eight days later, cells entered log growth phase.

结果：①多分化潜能细胞的原代培养情况：胎盘组织消化后获得细胞中仅有少量的贴壁细胞，经两周后逐渐形成扁平单层细胞，呈漩涡状生长或成簇生长，随着细胞密度的增加，胞体变得细长，形态类似成纤维细胞。

The deep cultivation-root cutting in before winter stage would increasethe weight and ratio of root system in middle and lower layer,the resultswould decrease the root redundancy.

冬前深耘断根可增加中、下层根系的重量和比率，降低根系生长冗余性，控制最高总茎数，提高成穗数及成穗率，使籽粒产量中来自花后光合器官输送的比例增加，从而使产量提高，因而在旱地高产麦田应推广冬前深耘断根。6研究了降水量对旱地高产小麦耗水规律及产量的影响，拔节前降水多且占比重高，土壤供水量及占比重少，拔节后降水量减少且占比重低，土壤供水量及占比重高，利于在旱地高产麦田形成高产。

The second approach ingredients: flour ingredients: scallions tender meat a little rapejiang suan heart a little practice: 1, warm water and soft PowerPhone and more and after a good 5-7 minutes Add basin line 2, scallions fine cut of meat Stand-slice 3, the line mian zhang sharing with good flour into a pie, a knife slip into a three-finger width of article 4, under the boiling water into the surface film will stretchjiang suan in 6,7 minutesru guo be mature plus nine patches salt, Add Rape Heart 7, points to the vinegarqi guo 8, surface-chip Add shallot sauce

第二种做法主料：面粉配料：香葱嫩油菜心姜蒜少许肉片少许做法：1、温水和面面和的软些和好后放入面盆行5－7分钟 2、香葱切细肉切片备用 3、将行好的面粉用擀面杖摊成饼状，用刀滑成三指宽的条 4、滚水下入姜蒜将面片抻入锅中 6、7分钟后待面片九成熟加盐，放入油菜心 7、点入香醋起锅 8、面片上放入香葱调味

Inseminated in 8-9 hrs after GVBD, the ova have the highest hatching rate (more than 86% in the concerned spawn).Based on the above insemination research of the oocyte maturation in vitro, recombinant opAFP-pUC19 was microinjected into the germinal vesicle of oocyte of maturation in vitro.

经过卵母细胞时期在生发泡中注射外源抗冻蛋白基因的体外成熟卵，其受精率（70～80%）和孵化率（38～75%）均低于对照组10～30%，在进行外源抗冻蛋白基因注射后授精孵出的小鱼长至半成体和成体鱼后，分别用斑点杂交和Southern blo-tting分析外源基因的整合。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。