后成的

Use constituent piece to stick mural way depart to foster Niu Er to become fiber cell above all, after classics generation is rarefied, fat simple way will be contained double the skin of the 4th acting ox that outside conveying carrier to turn into body, the particularity of person bacteriolysis enzymatic mammary gland that chooses number fosters becomes fiber cell, g/mLG418 chooses 500 μ 2 weeks, g418 halve continues to develop 3 generation of 2 ～, catch the cell after choosing to undertake PCR detects to turning next reach nucleus analysis.

首先采用组织块贴壁法分离培养牛耳成纤维细胞，经传代纯化后，脂质法将含有双重筛选标记的人溶菌酶乳腺特异性表达载体转入体外培养的第4代牛皮肤成纤维细胞，500μg/mLG418筛选2周，G418减半继续培养2～3代，然后对转染筛选后的细胞进行PCR检测及核型分析。

Used in shaping process of brick production , the treated clay will be plastic extruded by up stage then via vacuum room into down stage, estruding into solid or hollow clay column of required cross section and shape which then cut into greenbrecks by green breck cutter.

JKR40/40型双级真空挤砖机适用于砖瓦生产成型工段，可将经过处理的原料经过上级增塑挤出后落入真空室抽取空气后再经过下级挤成规定断面的实心或空心坯条，再经过切坯机制成一定规格的砖坯。

After the analysis it was concluded that the mean velocity fluctuations of the strata under Jidike Group in this working area could be divided into three zones: the minor velocity fluctuation zone in southern Dalaoba tectonic belt, the steep velocity fluctuation zone of the foreslope in middle YK structure and the zone from the bowtype thrusting tectonic belt in northern Qiulige to the minor velocity fluctuation zone in YK tectonic zone. On the basis of a good command of the regular patterns of the velocities in vertical and horizontal dimensions in the investigating area, the stack velocity was interpreted in a interactive mode by using the Depthteam Express modelling package of the Landmark system, converting the stack velocity into mean velocity by means of Dix formula. Based on the well drilling layering data, the velocity correction was executied to get the final corrected velocity model by using the Kriging Correction. By using this velocity model, we did the time to depth conversion and obtained the final depth structural map. After mapping by using the variable velocities, we came in for the display of the tectonic map of the seismic data.

经研究认为本区的吉迪克组以下层位平均速度变化可分成三个变化区域：南部大涝坝构造带速度变化平缓区；中部亚肯构造前缘斜坡区速度变化强烈区；北部秋里塔格弧形逆冲构造带前缘至亚肯构造带速度变化平缓区，在撑握工区速度纵、横向变化规律基础上，应用Landmark解释系统Depthteam Express速度建模软件包对迭加速度进行交互解释，利用DIX公式把迭加速度转换成平均速度，根据区内钻井分层数据采用克里金校正方法进行速度校正，得到最终校正后的速度模型，用此速度模型进行时深转换，得到最终的深度构造图，此次变速成图后，得到地震资料构造图显示。

Look from the content that informs against a letter, the person that inform against says is " blue sky lanneret " child, they and " blue sky lanneret " get acquainted with in sina UC chatting room, over,"Blue sky lanneret " impart to them fry a skill, after paying 10 thousand yuan of tuition each, became " blue sky lanneret "" close door child ", learn with him " fry a stunt ", formal courses by a definite date 2 weeks, after learning, often also can consult.

从举报信的内容看，举报者都称是"蓝天雄鹰"的弟子，他们与"蓝天雄鹰"在新浪UC聊天室中结识，在那里，"蓝天雄鹰"向他们传授炒股技巧，每人在交了1万元学费后，成了"蓝天雄鹰"的"关门弟子"，跟他学习"炒股绝技"，正式课程为期2周，学成后也可以不时请教。

The sending end when the use of pulse modulation approach will be a binary digital signal into a frequency modulation of the pulse sequence, and driver fired infrared tube to the form of light pulses sent receiver will receive the pulse of light conversion to electrical signals , and then, after amplification, filtering and other treatment to the demodulation circuit after demodulation, reduced to a binary digital signal after the output.

发送端采用脉时调制方式，将二进制数字信号调制成某一频率的脉冲序列，并驱动红外发射管以光脉冲的形式发送出去；接收端将接收到的光脉转换成电信号，再经过放大、滤波等处理后送给解调电路进行解调，还原为二进制数字信号后输出。

Firstly, the digital watermarking was transformed randomly, and then encrypted by chaos. The encrypted watermarking was transformed to one-dimensional row vector, and the pixel value was sorted. The coefficient of primitive image of stationary wavelet transformation was expanded to one-dimensional row vector, too, and then the sorted watermarking was embedded to the sorted low frequency and turned to two dimensions.

本算法先将数字水印图像进行置乱变换，然后将置乱后的水印图像进行混沌加密，将加密后的水印按行展开成一维行向量，并将像素值从大到小排序，将原始图像平稳小波分解得到的低频系数也按行展开成一维行向量，并按从大到小排序。

It was concluded that P2O5 was an effective nucleating agent for this glass system. The nucleation density of this glass system was too low to form tiny crystal after heat-treatment, when it contained no P2O5, and the major crystal phase was lithium silicate. Adding P2O5 resulted in major tiny crystal phase of lithium disilicate after heat-treatment.

结果发现P2O5是该玻璃体系的有效成核剂，未添加P2O5的玻璃体系成核密度低，热处理后不能形成微晶体，且主晶相为硅酸锂；添加P2O5使玻璃在热处理后形成以二硅酸锂为主晶相的微晶玻璃。

At the end of 12 and 18 weeks, rats were killed, taken the muscle of posterior limb, after separating the gastroenemius、soleus、plantaris, to prepare the frozen section of the each kind of muscle respectively to be confirmed to form the myopathy model by the histochemical stain.(3) RT-PCR、Western blot and immunohisto-chemistry stain were respectively used to detect the expression of NF-κB、MMP-2 and MMP-9 during different period.

2实验组用酒精给大鼠灌胃，对照组用含与酒精体积相等的生理盐水灌胃，两组均喂饲改良的全营养高脂饲料，于12周及18周后，取后肢肌肉，在分离出腓肠肌、比目鱼肌、跖肌后，将各类肌肉均分别地制备成冰冻切片和石蜡切片，经组织化学染色确认成模。

To understand the infectivity by porcine endogenous retrovirus with porcine skin fibroblast cell in vitro and in vivo, porcine skin fibroblast cell established by our laboratory were co-cultured with neo/HEK293 cell for the infection of RERV in vitro, and were subcutaneously transplantated to SCID (severe combined immuno-deficiency) mice for the infection of PERV in vivo, laying the foundation for valuation of biologic safety of xenotrans-plantation. The event of neo/HEK293 cells infected by PERV occurred during co-culture of porcine skin fibroblast cells with neo/HEK293 cells, expanding the rang of the infection of porcine endogenous retrovirus. Afterpig cells transplantated subcutaneously in SCID mice, the microchimerism (78.57%) of pig cells occurred widel, and there was phenomena of integration of PERV provirus (85.71%) in several organs or tissues remote from the injected sites, indicating infection of PERV in SCID mice in vivo. yet, there is no evidence of active viral replication in analysis of PERV env RNA of these tissues or organs.

为了解猪皮肤成纤维细胞PERV在体外和体内的感染性，通过建立猪皮肤成纤维细胞系，将所建细胞系与人胚胎肾293细胞体外共培养，并移植于严重联合免疫缺陷鼠皮下进行猪皮肤成纤维细胞PERV的体外和体内感染性实验，结果表明，猪皮肤成纤维细胞与人胚胎肾细胞共培养过程中，猪内源性逆转录病霉感染人胚胎肾细胞，进一步证实和拓宽了猪细胞PERV感染人细胞的范畴；猪皮肤成纤维细胞移植SCID鼠皮下后，导致SCID鼠发生猪细胞微嵌合(78.57%)和PERV在体内感染(85.71%)并且波及远离移植部位的多种组织或器官，但是并未检测出SCID鼠组织中表达PERV env RNA。

In order to confirm this suspect, we choosed a cultured rat granulation tissue fibroblasts model in vitro and applied RT-PCR and cell-ELISA technology to detect the changes of firbroblasts intrinsic EGF, bFGF, TGFβ-1 with their receptors gene expressions and protein synthesises after stimulated by SP. Our purpose was to explore the possible effects and patterns imposed by SP on fibroblasts intrinsic growth factors and their receptors expressions, which maybe offer theoretical basis for promoting wound healing via improving nervous functions and regulating neuropeptides secretions.

为进一步验证这一推测，同时排除在体多因素干扰，我们采用了一种大鼠肉芽组织成纤维细胞体外培养模型，采用RT-PCR与细胞ELISA技术，检测SP刺激成纤维细胞后，成纤维细胞内源性EGF、bFGF、TGFβ-1及其受体基因表达和蛋白合成的改变情况，探讨SP对成纤维细胞内源性生长因子及其受体表达的影响以及方式，以期为经由改善神经功能、调节神经肽分泌途径促进伤口愈合提供理论依据。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。