后成的

Results: Fifty-two of 66 patients with unilateral fractures were, after 9–13 years, rated as excellent/good according to the Green and O'Brien score as modified by Cooney et al.

结果：66例单侧桡骨无故骨折患者，有52例在伤后9－13年GOBC评分为优良，AO骨折分类与结果无关，相当多的患者仍遗留骨折移位：背侧成角（小于60岁者，平均13°，大于60岁者，平均18°），与伤时相比桡骨存在短缩（小于60岁者，平均2mm，大于60岁者，平均3mm），5例患者复位后仍存关节面缺损（1－2mm），但是仅有1例患者后来发展成了轻度的骨性关节炎。

But in SD rats, cardiac muscle fibers were fractured seriouly , contracted to wavilness obviously, then muscle fibers fracture were contracted like entasia by the hypoxia time increased , injury intensify , all the changes refered SD rats compensatory mechanism can not be transferred in this hypoxia degree. 3. Exposed to 4% oxygen concentration, the cardiac muscle ultrastructure of Gansu zokors had serious injury after hypoxia 2h.

在4%氧浓度的低氧耐受实验中，甘肃鼢鼠在低氧2，4h后，心肌纤维收缩明显呈波浪状，低氧时间延长至6，8和10h后，心肌显微波浪状收缩由强逐渐变弱，并逐渐恢复至正常状态，说明鼢鼠心肌在6h以后对严重缺氧环境开始代偿性适应；SD大鼠2h组心肌纤维断裂严重，收缩加强成明显的波浪状，随着低氧时间得增加，4h组呈严重成挛状收缩，损伤加剧，提示SD大鼠在这一氧浓度下已经无法正常调动其代偿机制。3。

The material for formation of the granitoids isderived from mixing sources of crust and mantle, with decreasing in crust-derived componentsfrom early to late.5. The tectonic setting for formation of the Mesozoic granitoids in the region isdefined as a post-collisional setting. The occurrence of a small part of rocks with characteristics ofcollisional or anorogenic granitoids provides evidence for the continuous process oftransformation of tectonic regime from collisional through post-collisional to anorogenic.6. Themultiple cycles of mantle-derived magma underplating caused by the multiple stages oflithospheric thinning in the Mesozoic result in the partial melting of middle to lower crust orcrust-mantle syntexis to form magmas. The crystallization of these magmas emplacing afterfractionation is responsible for the formation of the Mesozoic granitoids in this region.

形成花岗岩类的成岩物质来自壳幔混源。5、河南省西部地区中生代花岗岩类成岩构造环境为碰撞后环境，部分岩石具有碰撞期或非造山期花岗岩的某些特征，表明碰撞后阶段既是碰撞造山过程的延续，又是向非造山环境的构造转换时期，因而其岩浆活动在一定程度上仍然继承了碰撞前消减时期和碰撞时期已经活化的岩浆源区性质。6、研究表明，区内中生代多阶段的岩石圈减薄作用引发多旋回的幔源岩浆底侵以及中下地壳的部分熔融或壳幔同熔，由此形成的岩浆经分异演化后侵位可以形成区内的中生代花岗岩类。

Shows the end of Henan, Shaanxi Opera and after the introduction Puzhou Clapper, and local folk songs, made minor combination; one that transferred from the Northern Song direct the development of cable made of string; a folk concert in Henan is art, particularly since the Ming Dynasty later, in the Central Plains region based on the prevailing fashion Xiao Ling, absorbing "string rope" and other artistic achievement evolved.

一说明末秦腔与蒲州梆子传入河南后，与当地民歌、小调相结合而成；一说由北曲弦索调直接发展而成；一说是在河南民间演唱艺术，特别是自明朝中后期，在中原地区盛行的时尚小令基础上，吸收"弦索"等艺术成果发展而成。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

General 薄本 first bookbiding after the die-cutting or trimming the better; and thick this processing method vary, there's plenty of stepwise cut after the finished product, some of them are single-page die cutting out heterotype bookbiding or paper again.

一般薄本采用先装订后模切或冲切的方法比较好；而厚本的加工方法有所不同，有的是成品后分段冲切而成，有的是模切出异型单页再装订或裱糊而成。

The forced expression of myogenin could induce NIH3T3 fibroblast to differentiate into myogenesis, it was identified as a pair of bands on SDS-PAGE. The phosphorylated form implies a significant structural change in the myogenin protein, which is expected to affect its function at post-translational level.

强制性表达外源性生肌素可启动NIH3T3成纤维细胞分化，进入成肌过程。pCD1-myog转染后的蛋白印迹带型迁移率发生改变，说明磷酸化导致生肌素蛋白结构的明显变化，并可能在翻译后水平影响其功能。

RESULTS: After differentiation of human adherent myoblasts by neural induction medium, no cells with a neural cell morphology (ie., small, refractile cell body with dendritic cell extensions) were seen. All remaining myoblasts cultured with neural induction medium, myoblasts with proliferation medium and myotubes with differentiation medium containing 20 mL/L HS were positive for β Tubulin Ⅲ. C2C12 myoblasts were negative for β Tubulin Ⅲ. In contrast, all the above cells were negative for the markers Neurofilament Mr 68×103 and GFAP.

结果：用诱导分化液作用后，未见小的、伴有突起的放射状形态的神经细胞；抗β Tubulin Ⅲ对经神经元胶质细胞诱导分化液作用后的人成肌细胞、增殖培养液培养后的各代人成肌细胞及仅含20 mL/L HS分化液分化形成的肌管细胞染色均为阳性； C2C12细胞β Tubulin Ⅲ抗体染色阴性；上述所有细胞抗Neurofilament Mr 68×103和抗GFAP染色均为阴性。

The fibrils of three levels,Which were macrofibrils of several tens micron in diameter,fibrils feW micron and microfibrils about 0.3 micron,were observed simultaneously with banded texture usingpolarized microscope.After annealing,fibrils in different levels relaxed to differentmorphology.Macrofibrils still oriented along shear direction,but fibrils relaxed to helical form,and microfibrils relaxed to waveform therefore the banded texture canbe seen.The smaller the diameter of fibrils was,the more easily the fibrils relaxed.Ori the other hand,the banded texture decoration of annealed unoriented filmsrevealed directly the distribution of directors within and out of domains and thevariation of directors on boundaries.

微纤分三个层次，即直径分别为数十微米的微纤束、数微米的微纤和0.3微米的亚微纤，退火后三个层次的微纤松弛成不同的形态，微纤束仍笔直沿剪切方向取向，微纤松弛成螺旋形，而亚微纤松弛成波浪形从而观察到条带织构：微纤直径越小越易松弛，另一方面，非取向态织构退火后由亚微纤形成的条带装饰结果直观地揭示了微区内外分子指向矢的分布和它们在边界上的变化。

Reefs, reef-mounds and lime mud mounds are all carbonate buildups, and the reefs and banks are important targets for petroleum exploration The reefs, banks and mounds should be divided from each other not only in theory, but also in exploration practice, because of so many differences in sedimentary compositions, fabrics, environments, distribution in time and space, and the characteristics of petroleum geology If the original reef or bank itself was fine reservoir rocks, then it would become a better reservoir after the reservoirconstructive diagenesis The formation of a high quality carbonate reservoir is controlled by two factors: the sedimentary microfacies and the reservoirconstructive diagenesis The first is the basis for the formation of a high quality reservoir, and with the high energy reefbank facies as the best; the latter includes the cataclasis, the dolomitization and the karstification The dolomitization and karstification are the best for the formation of a high quality reservoir The karstification has the widest distribution, profoundest effect, and plays a vital role in the high quality reservoir forming The karst types are quite rich in the limestone reef or bank reservoir, including synsedimentary karst, penesedimentary karst, supergene karst and hydrothermal karst, and so does the dolomitization reef or bank reservoir The formation mechanism of synsedimentary karst and penesedimentary karst is related to the short time exposing and leaching of the cyclical highfrequency shallowupward sequence when the reefs or banks are forming The supergene karst is controlled by the exposing and leaching of tertiary cyclic sequence type I interface after the reefs or banks forming Thus, there developed a great deal of secondary corrosion pores, corrosion fractures and huge caves besides some original pores remained in the reef and bank reservoir Scarcely there are any original effective pores in reefmounds and lime mud mounds, especially in senkes among mounds, but secondary pores could be formed by karstification, and there would likely develop source rocks with high organic abundance

礁、礁丘、丘同属碳酸盐建隆，礁、滩是重要勘探目的层。但各自在沉积组分、组构、环境和时空分布，以及油气地质条件上迥然不同，因而无论从理论还是勘探实践上，都有必要将其各成体系划分开来。礁滩自身为好储集岩，当建设性成岩作用叠加后可形成好的储层。碳酸盐岩优质储层形成，主要受沉积微相与建设性成岩作用两大因素控制。前者是基础，并以高能的礁滩相带为最好；后者主要包括破裂、白云石化和溶蚀三大类，并以白云石化或溶蚀为最优，其中的溶蚀分布最广泛、影响最深刻而对优质储层形成起决定性作用。礁滩储层中的古岩溶类型最为丰富多彩，包括同生期、准同生期、表生期和埋藏期热流体岩溶的所有类型，白云岩化礁滩体也不例外。其中，同生准同生期岩溶的形成机制与礁滩沉积时高频层序旋回向上变浅序列的短暂暴露淋溶有关，表生期岩溶则受控于礁滩沉积后三级层序旋回Ⅰ型界面的暴露淋溶。由此，导致礁滩储层中除了可以也是唯一保存原生孔隙外，还发育了次生溶蚀孔洞缝和大型溶洞。礁丘、灰泥丘尤其是丘间洼地中发育高有机质丰度烃源岩；它们自身没有有效的原生孔隙，但岩溶作用可以形成次生孔隙。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。