后成体

GFP gene eukaryotic express plasmids were transfected into ES cells. The cells were observed under the inverted fluorescent microscope. The clones which expressed the powerful green fluorescence were chosen to be named ES-GFP cells and cultured continuously. The examination of ES cell totipotent including Alkine phosphatase staining, embryonic body formation in vitro and teratomas formation in nude mice was implemented.

1。采用脂质体方法将GFP质粒转染ES-D3细胞株，经筛选后倒置荧光显微镜观察细胞形态及荧光状态，挑取荧光最强的克隆，命名为ES-GFP细胞，进一步扩增培养，观察细胞生长及荧光表达情况，行细胞分化全能性鉴定包括碱性磷酸酶（alkine Phosphtase，AKP）染色、体外胚胎体（embryonic body，EB）形成实验及裸鼠体内成瘤实验。2。

Methods: Polytetrafluoroethylene and polypropylene film electrets were prepared by constant voltage corona charging at room temperatures. The charge-store stability of PTFE and PP were studied by measuring isothermal surface potential decay after different dose of ultraviolet radiation.

用常温恒压电晕充电系统将聚四氟乙烯和聚丙烯两种材料极化制备成驻极体；用补偿法测量不同剂量的紫外照射后驻极体的表面电势。

In this paper, the flax yarn will be used as the continuous reinforcement and the Polypropylene multifilament will be used as thermoplastic resin matrix. The two things will be made into a pp/flax covered yarn, which are ready to be woven into a plain fabric. The flax/pp composite board is accomplished by hot-pressing molding. Some other composite boards are also made, whose flax yarn haven been pretreated.

本课题将以亚麻纱线作为连续状增强体，聚丙烯复丝作为基体；采用包覆纱的浸渍工艺，制作了pp/亚麻包覆纱后浸料；再将包覆纱制成机织平纹布作为预成型件；通过热压成型工艺制作成复合材料板材；同时还制作了对亚麻纱线进行碱处理和偶联剂处理的复合材料板材。

The subsequent larval and nymphal progeny were applied onto the calves infected with Theileria annulata in order to investigate whether Hy. a. anatolicum were infected by both Theileria annulata

结果显示：巴贝斯虫未定种首先在蟀的肠道内发育，经一系列未知的变化后，在蟀饱血脱落5天内形 2 摘要成运动体，随后，几乎同时可在肠道、血淋巴、唾液腺及卵巢内发现大裂殖体

Methods Sixteen adult Sprague-Dawley rats were enrolled, from which anticoagulative erythrocyte suspension was made, and was incubate with equivalent volume of 1 time,2 times,4 times and 8 times of clinical therapeutic blood concentration of different local anesthetics respectively. Erythrocyte-yeast rossete and Tumor-erythrocyte rosette rate were measured.

选择成年SD大鼠16只，取抗凝血制备成所需浓度的红细胞悬液，与等体积的临床血药浓度、2倍、4倍及8倍临床血药浓度的各研究麻醉药物孵育后，加入补体致敏酵母菌或用自身血浆作为补体来源致敏的肿瘤细胞，测定红细胞、酵母菌花环率和肿瘤细胞-红细胞花环率。

Its dextrorotatory substant is an antidepressant which does not have the helminthic effect.

所不同的是四咪唑为外消旋体，左旋咪唑是四咪唑经过化学方法处理后拆分而成的左旋体。

The results indicate that the microstructure of laser remelted coating is both homogen eous and dense, the main phases of laser remelted coating are AlFe, CrFeNi and so on.

结果表明：激光重熔后涂层组织均匀、致密，主要由AlFe固溶体、 CrFeNi奥氏体等相组成，而且涂层与基体形成了良好的冶金结合。

Methods Human hemangioma endothelial cells cultured in vitro were injected hypodermically into nude mice(BALB/c nude mice). The volume of in the transplanted tumor was measured in 60 days. Samples were taken from survival tumor and stained immunohistochemically by CD31 and Ki-67 before subjected to histopathological examination.

将体外培养的血管瘤内皮细胞通过注射的方式植入裸鼠(BALB/c nude mice)皮下，测量不同生长时期瘤体体积变化，观察至60天后进行病理学光镜检查，并通过血管内皮细胞单克隆抗体CD31、细胞增殖标记抗体Ki-67免疫组化染色研究所成瘤体的同源性及增殖活性。

The method adopts the following steps: after mixed, phosphorus source, silicon source, aluminum source, an organic template agent, water and a precursor of the SAPO molecular sieve are hydrothermally crystallized for at least 0.1 hour at the temperature ranging from 110 to 260 DEG C to obtain the SAPO molecular sieve; wherein, the preparation method for the precursor of the SAPO molecular sieve has the following steps:, the molar ratio is 0.03 to 0.6 R:(Si0.01 to 0.98: Al 0.01 to 0.6: P0.01 to 0.6): 2 to 500 H2O; wherein, R stands for mixture solution of raw materials which is blended by the phosphorus source, the silicon source, the aluminum source, the an organic template agent and water of the template agent;, the mixture solution of raw materials prepared in step reacts at the temperature which is at least 50 DEG C lower than the crystallization temperature for at least 0.1 hour.

本发明通过采用包括以下步骤：将磷源、硅源、铝源、有机模板剂、水和SAPO分子筛前驱体混合后在110～260℃的温度下水热晶化至少0.1小时获得SAPO分子筛；其中SAPO分子筛的前驱体的制备包括以下步骤：将摩尔配比为0.03～0.6R∶(Si0.01～0.98∶Al0.01～0.6∶P0.01～0.6)∶2～500H 2 O，式中R代表模板剂的磷源、硅源、铝源、有机模板剂和水混合成原料混合液；将步骤制备的原料混合液在低于晶化温度至少50℃的温度下反应至少0.1小时的技术方案较好地解决了该问题，可应用于含氧化合物制烯烃催化剂的制备过程。

Results Following orchiectomy 7d,the amount of CGRP-immunoreactive nerve fibers in the AP of the rat increased significantly, with the tortuous varicose fibers more densely distributed than that of NOR and the amount of varicosities increased significantly, while the thick fibers were more thicker than that of NOR,and many thin fibers were sent out from the thick fibers, and the varicosities were more larger.

结果 睾丸摘除7d后，大鼠垂体前叶CGRP免疫反应神经纤维发生了显著变化，表现为膨体型纤维较正常组稠密，分支增多，迂曲成片，盘绕成网，分布更加广泛，膨体数量显著增多；粗纤维较正常组粗，且发出许多迂曲盘绕的细纤维，膨体形态变大。t检验显示，OX组与NOR组垂体前叶内CGRP免疫反应神经纤维的平均面积密度差异显著，OX＋V与NOR组差异显著。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。