后成体

Results一. Culturing and identification of human conjunctiva cells1. morphous of conjunctiva cellsCells were harvested by tissue cultivation after digestion. Ponderosus cells adhered after 48 hours. Cells were shown in shape of round, ellipse and polygon. Cell body was loose and lucency with nucleus in center, cell membrane was also clearly seen. Cells were arranged in inlay and fuse in film after 12-14 days.2. Immunochemistry testNomogeneous immunochernical positive granules against CK13 were observed in endochylema of human conjunctiva cells.3. growth curve of conjunctival cellsSubcultured cells were aged and feeble after 5th passage.

结果一、正常人结膜细胞的培养与鉴定1、活体细胞的形态消化后组织块培养法：48小时后见大量上皮细胞贴壁，细胞不规则呈圆形、椭圆形、多边形，细胞体肥大透亮，细胞核位于中央，胞膜清楚，约12-14天后细胞呈镶嵌状排列，融合成膜状。2、免疫组化鉴定培养的人结膜上皮细胞CK13免疫组化染色见阳性颗粒均匀分布于胞浆中。3、培养结膜细胞生长曲线传代培养多数样本于P5代以后开始出现衰老征象。

Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有按摩明显的阻抑作用，加药48h后增殖的抑制率可达43%以上；(2)ATP处理的U937细胞周期发生改变，G1期细胞数按摩明显增多，ATP作用24h时G1期前出现亚二倍体峰——凋亡峰，凋亡细胞数为3.4%，作用48h时凋亡细胞数增多为22.7%；(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜，逐渐向核膜外移动，进入胞浆内再移向质膜内外面，紧贴质膜外面再逐步脱离细胞体，进入细胞基质中，成为游离的凋亡小体。

Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有明显的阻抑作用，加药48h后增殖的抑制率可达43%以上；(2)ATP处理的U937细胞周期发生改变，G1期细胞数明显增多，ATP作用24h时G1期前出现亚二倍体峰——凋亡峰，凋亡细胞数为3.4%，作用48h时凋亡细胞数增多为22.7%；(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜，逐渐向核膜外移动，进入胞浆内再移向质膜内外面，紧贴质膜外面再逐步脱离细胞体，进入细胞基质中，成为游离的凋亡小体。

The results show that a MgSnO3·H2O conversion film is formed by treatment in the stannate solution at 90℃, and the immersion test and potentiodynamic polarization show that the corrosion resistance of the AZ91D alloy is improved to some extent.

结果表明：锡酸盐转化处理后合金表面形成了以MgSnO3·H2O为主要成分的转化膜，可对合金起到一定的防护作用；转化膜由细小的球形颗粒密积而成，颗粒之间存在间隙，它可以为化学镀镍的前处理过程提供良好的吸附条件；转化膜上的化学镀镍层组织致密、无缺陷， MgSnO3·H2O转化膜在镀镍层与基体之间起到过渡作用，镀层的磷含量达到9%，与基体结合良好；在3.5% NaCl (pH=7)溶液中的动电位极化测试表明，镀镍以后的合金在阳极极化过程中发生了明显的钝化，耐腐蚀性能进一步提高，对基体起到了较好的防护作用。

Experiment shows that both the dry plate pressure drop and the wet plate pressure drop increase with the increase of the kinetic energy factor in plate hole but have no influence of the height of cap. The wet plate pressure drop linearly ascends as the clear liquid height increases. Liquid lift-up increases at first then decreases with the increasing of the kinetic energy factor in plate hole, increases with the clear liquid height increasing and the height of cap decreasing, but as the kinetic energy factor in plate hole increased, the influence of height of cap will gradually decreased.

实验表明干板压降、湿板压降均随板孔动能因子的增大而增大，不受罩体高度变化的影响，湿板压降随清液层高度的增大而增大，成正比关系；液体提升量随板孔动能因子的增大先增大后减小，随清液层高度的增大而增大，随罩体高度的增大而减小，但随板孔动能因子的增加，罩体高度的影响逐渐变小；漏液量随板孔动能因子的增大迅速减小，随清液层高度的增大而增大，但接近漏液点时清液层高度的影响不明显。

The results indicated that the typical apoptosis was induced by PRRSV in lungs and uteri. The ultrastructural changes showed different characterizations at different stages of the infection. During the early stage from 8th h to day 3 postinfection,the apoptosis cells shrank,the cytoplasm concentrated,the chromatin condensed,the kernel disaggregated and the endoplasmic reticulum dilated. During the middle stage from day 5 to day 9 postinfection,the apoptosis cells evidently shrank,the cell membranes protu berated,the plastosome manifolded,and apoptosis bodies were found. During the last stage from day 10 upwards postinfection,the apoptosis bodies degenerated and disappeared,and no inflammation occurred. Few apoptosis cells were necrotic.

结果表明，PRRSV可诱导宿主肺和子宫发生典型的细胞凋亡，表现为细胞的超微形态结构随着病毒感染进程出现不同的特征性变化，早期（感染后第8 h至第3 d）凋亡细胞多表现为细胞体积缩小，细胞质密度增强，染色质浓缩，核仁解体，内质网扩张；中期（感染后第5 d至第9 d）凋亡细胞体积显著缩小，细胞膜突起，线粒体增多，有凋亡小体形成；晚期（感染后第10 d以后）凋亡细胞形态多表现为凋亡小体降解和消失，少数凋亡细胞在凋亡晚期表现为坏死细胞。

The replicons were detected and characterized by RT-FUR. IFA. Renilla Luciferase assay system and real time RT-FUR. respectively. Results It was shown that SLB2 mutant did out significantly affect the translation of the input RNA.

将突变体（包括相关的阳性和阴性对照复制子）分别线性化并体外转录成RNA后，取等量各转录体RNA，以脂质体法分别转染BHK-21细胞。

Methods:Human CD80 cDNA was transfected into B16 melanoma cells by lipofectin-mediated gene transfer before the expansion of tumor cells were tested by MTT method in vitro;C57BL/6 mice were inculated subcutaneously either mock-transfected B16 cells (B16-neo)or CD80-transfected B16 cells (B16-CD80),then the latency,survival times and tumor mass growth were investigated.The lymphocytes were examined for both proliferation indices and specific cytotoxic activity by MTT method and improved LDH-releasing method,after syngenic Mixed Lymphocyte tumor cultures.

通过脂质体介导将CD80基因导入小鼠黑色素瘤B16细胞后，利用SABC法检测CD80的表达；MTT法测定肿瘤细胞体外增殖能力；将肿瘤细胞接种至同源小鼠皮下后观察成瘤期、荷瘤小鼠存活期及肿瘤结节生长速度；在同源淋巴细胞肿瘤细胞混合培养后，通过测定淋巴细胞增殖指数和CTL杀伤活性检测肿瘤细胞的免疫原性。

In order to remedy this kind of shortage, this paper presents a quick volume rendering method, for the finite element data field of dam seismic response, choices the slicing direction relying on the element surface which normal line has the smallest included angle with the view direction, slices elements to many quadrilaterals, blends the colors of these quadrilaterals by using OpenGL blending technology, and then superimposes all elements one by one.

为了弥补传统体绘制静止画面不能明确判断场值集中部位的不足，提出一种快速体绘制算法，针对大坝地震反应有限元计算数据场，根据每个单元法线与视线夹角最小的表面来选择剖切方向，将单元剖切成多个四边形面后，对单元的绘制顺序进行合理排序，利用OpenGL图形库函数进行颜色融合，再将各个单元依次叠加，从而生成了三维数据场的体绘制图。

The results show that: 1 supplementation of protein to maturation media improves cumulus expansion in vitro compared to the protein-free control, but cumulus expansion is not necessarily related to oocyte nuclear maturation in pigs, and cumulus expansion is not the criterae for determination of nuclear maturation of pig oocytes, but only the exclusion of the first polar body; 2 exposure of pig COCs to hormone supplements for 23-24 hours improved cumulus expansion but had no significant effect on nuclear maturation compared to that for 46-48 hours; 3 under our research conditions, supplementation of different proteins into different maturation media has different effects on porcine oocyte nuclear maturation, but has no significant effect on subsequent embryonic development after IVF; 4 the nuclear maturation rates of pig oocytes matured in mTCM+pFF and mNCSU+pFF are superior than that in mNCSU+FCS; 5 different maturation media have no effect on pig oocyte cumulus expansion and subsequent embryonic development after IVF.

结果显示：(1)在成熟液中添加蛋白质可以加强卵丘细胞的扩散，但猪卵母细胞的核成熟与其周围的卵丘细胞扩展没有必然的联系，卵丘细胞扩散或成放射状不宜作为猪卵母细胞核成熟的标准，只有排出第一极体才能作为猪卵母细胞核成熟的标志；(2)在猪COCs的46-48小时成熟培养的后23-24小时阶段去除成熟液中的激素不但可以保证卵母细胞的核成熟率，而且可加强卵丘细胞的扩散；(3)在现有实验条件下，在mTCM和mNCSU中添加10％pFF与在mNCSU中添加10％FCS相比可获得较高的猪卵母细胞核成熟率；(4)在不同的成熟液中添加不同的蛋白质对猪卵母细胞核成熟率的影响效果不一样，但对体外受精后的早期胚胎发育影响不明显；(5)成熟液种类对猪卵母细胞的卵丘细胞扩散和体外受精后的早期胚胎发育无显著影响。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。