后成体

Methodsafter total decompression of the spinal nerve through posterior medline approach, discectomy of the slipping segment was performed with a circular saw with diameter of 1.0 centmeter. under direct visualization through the hole of the circular saw, the disc tissue including cartilage of upper and lower end plates was removed with a long handle curette.

方法后正中入路脊髓神经根彻底减压后，用直径1.0 cm环锯摘除滑脱椎体间的椎间盘，然后通过环锯孔直视下彻底清除椎间盘髓核组织，用长柄刮匙刮除椎体上下终板软骨，使骨面成鱼鳞状。

The results show that the microstructure of ventra1 scale for python was composed of micro-convex and plank structure that was periodically arranged, it was mensurable and could be described by 9 characteristic parameters. Its surface was different in their forward and backward lean angle. The frictional coefficient of the ventra1 scale had closely relationship with moving direction, it decreased with the increasing load. The value of the frictional coefficient was about 0.07. The frictional coefficient while python moved backward and laterally were equal, and that was 33% more than that moved ahead. The frictional force of the ventra1 scale surface was caused by mechanical tooth function of micro-convex, acting intermolecular force and material's elastic hysteresis. The difference of forward and behind bevel for micro-convex was the anisotropy reason of frictional force.

结果表明：腹鳞表面的微观结构由指状微突体和板结构部分周期排列而成，其结构可用9个特征参数定量描述；腹鳞表面摩擦力由分子作用力、表面微突体的犁沟力、楔形作用力以及材料弹性滞后共同引起；腹鳞表面的摩擦系数在0.07左右并与运动方向有关，摩擦系数随载荷增加而减小；后向运动及左、右侧向运动时摩擦系数基本相等，比前向运动时高33%左右；腹鳞表面微突体不同方向上倾斜角度的差异是引起摩擦各向异性的主要原因。

SHRIMP technique is applied to the age determination of Shuiquangou alkalic complex. Zircons from monzonite and syenite of the complex in the Dongping and Hougou gold mining district are dated at age of (390± 6) Ma and (386±6) Ma, respectively with U-Pb method. Rock-forming times of the complex is confidently constrained in the Late Caledonian-Early Hercynian period.

采用先进的SHRIMP测年技术对河北水泉沟碱性杂岩体进行了年龄测定，获得东坪金矿区水泉沟岩体二长岩锆石U-Pb年龄为(390±6)Ma，后沟金矿区水泉沟岩体正长岩年龄(386±6)Ma，比较可信地确定了水泉沟岩体的成岩时代为晚加里东期－早海西期。

Because it is a hard work to refold the denatured inclusion proteins correctly, especially for those which contain several disulfide bonds. the results?repeatability of some activities is not very perfect.

纯化包涵体后，将产物变性成可溶形式，然后用Ni-NTA对表达产物纯化，SDS-PAGE检测为一条带，包涵体的复性是一项非常困难的工作，尤其是对含有多对二硫键的蛋白质，因此复性后冻干，测它的某些活性时，结果的重复性不太理想。

Working Principle Transmitter is composed of sensor and electronic element circuit, pressure of measured medium directly applies on sensor s ceramic diaphragm, force diaphragm to displace tinily in proportion to pressure of medium, under normal working condition, diaphragm displace no more than 0.025mm, electronic circuit detects this displacement, and convert it into standard industrial measuring signal i.e.

工作原理变送器由传感器与电子元件线路组成，被测介质的压力直接作用于传感器的陶瓷膜片上，使膜片产生与介质压力成正比的微小位移，正常工作状态下，膜片最大位移不大于0.025mm，电子线路检测这一位移量后，即把这一位移量转换成对应于这一压力的标准工业测量信号（4~20）mA。超压时膜片直接贴到坚固的陶瓷基体上，由于膜片与基体的间隙只有0.1mm，过载时膜片的最大位移只能是0.1mm，所以从结构上保证了膜片不会产生过大变形。

The screw entry point was located at the tip of transverse process. The average inclination angle should be 10°-20° headward and 30°-40° toward neutral line respectively. The screw was inserted and entered into the vertebral body along the external cortex of pedicle. The integrity of cortical bone around screws, the distance to segmental blood vessels and the penetration of cortex were observed after operation to evaluate the accuracy and safety of screw placement. Among 160 thoracic pedicle screws that were implanted in 34 cases, 148 screws (92.5%) were accurately inserted inside the costotransverse unit, and 12 screws (7.5%) were misplaced according to the image of X-ray and CT thin-slice scan.

应用椎弓根外内固定技术2004/2007年在中山大学附属第三医院骨科治疗34例患者，固定前采用CT加密扫描测量进钉点、进钉点至椎体前缘的深度、进钉角度和直径，固定中椎弓根钉入点为横突尖，进钉方向为平均向头侧倾斜10°~ 20°、与中线成角30°~40°，顺椎弓根外侧皮质进入椎体，固定后CT加密扫描观察螺钉周围的皮质骨是否完整、是否靠近节段血管、是否穿透皮质骨，评价置入钉的准确性和安全性。34例患者共置入160枚胸椎椎弓根螺钉，固定后CT加密扫描和X射线片观察到148枚(92.5%)螺钉置入准确，12枚(7.5%)螺钉发生错置。

From 2004 to 2007, 34 cases were treated with extrapedicular screw fixation in Department of Orthopaedics, Third Affiliated Hospital of Sun Yat-sen University. The screw entry point, angle, depth and diameter were measured and defined on the thoracic vertebrae pedicle by CT thin-slice scan in standard prone for all cases before operation. The screw entry point was located at the tip of transverse process. The average inclination angle should be 10°-20° headward and 30°-40° toward neutral line respectively. The screw was inserted and entered into the vertebral body along the external cortex of pedicle. The integrity of cortical bone around screws, the distance to segmental blood vessels and the penetration of cortex were observed after operation to evaluate the accuracy and safety of screw placement.

应用椎弓根外内固定技术2004/2007年在中山大学附属第三医院骨科治疗34例患者，固定前采用CT加密扫描测量进钉点、进钉点至椎体前缘的深度、进钉角度和直径，固定中椎弓根钉入点为横突尖，进钉方向为平均向头侧倾斜10°~20°、与中线成角30°~40°，顺椎弓根外侧皮质进入椎体，固定后CT加密扫描观察螺钉周围的皮质骨是否完整、是否靠近节段血管、是否穿透皮质骨，评价置入钉的准确性和安全性。34例患者共置入160枚胸椎椎弓根螺钉，固定后CT加密扫描和X射线片观察到148枚(92.5%)螺钉置入准确，12枚(7.5%)螺钉发生错置。

Results Infected by this peptide, cell viability decreased 28.9%. Under light microscope, cells were shrinked and rounded, many cells were divorced from plate wall, some neuraxon shortened and broke. Apoptotic cells which nucleolus shrinked and rounded could be coloured orange by fluorescent colouration. Under electron microscope, chromatin gathered along the inside of the nuclear membrane, vacuole bodies appeared. Apoptotic peak was detected by flow cytometry and the ladder band appeared in DNA electrophoresis.

结果：细胞接触肽段后存活率下降28.9％；光镜下可见细胞贴壁不良，胞体缩小，细胞突起断裂缩短；荧光染色可见细胞突起缩短、胞核固缩、胞质染成橘红色的凋亡细胞；电镜下可见胞质中出现空泡样结构，细胞染色质浓集于核膜内侧并裂解成碎块状；流式细胞仪检测细胞出现亚二倍体峰，DNA电泳出现梯状带。

With the increase of cell density, soma was slenderer and slenderer and like fibroblast.② Growth curves of PDMCs: Growth latency ranged from 2 to 8 days after cell inoculation. During this period, adherence was observed gradually but not obviously amplified. Eight days later, cells entered log growth phase.

结果：①多分化潜能细胞的原代培养情况：胎盘组织消化后获得细胞中仅有少量的贴壁细胞，经两周后逐渐形成扁平单层细胞，呈漩涡状生长或成簇生长，随着细胞密度的增加，胞体变得细长，形态类似成纤维细胞。

In order to confirm this suspect, we choosed a cultured rat granulation tissue fibroblasts model in vitro and applied RT-PCR and cell-ELISA technology to detect the changes of firbroblasts intrinsic EGF, bFGF, TGFβ-1 with their receptors gene expressions and protein synthesises after stimulated by SP. Our purpose was to explore the possible effects and patterns imposed by SP on fibroblasts intrinsic growth factors and their receptors expressions, which maybe offer theoretical basis for promoting wound healing via improving nervous functions and regulating neuropeptides secretions.

为进一步验证这一推测，同时排除在体多因素干扰，我们采用了一种大鼠肉芽组织成纤维细胞体外培养模型，采用RT-PCR与细胞ELISA技术，检测SP刺激成纤维细胞后，成纤维细胞内源性EGF、bFGF、TGFβ-1及其受体基因表达和蛋白合成的改变情况，探讨SP对成纤维细胞内源性生长因子及其受体表达的影响以及方式，以期为经由改善神经功能、调节神经肽分泌途径促进伤口愈合提供理论依据。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。