后成体

Curtain plate stainless steel plate, spray nozzle for the spray nozzle to capture, exhaust fan with explosion-proof fan.3, chamber body assembly plate connection between the use of bolts, installation, removal, fast and convenient.4, configuration, special explosion-proof spray room lighting, interior lighting sufficient light.5 mist treatment with water curtain, shower folding capture plus maze can efficiently remove the paint mist.6, circulation pump with an area of small, simple installation and pipeline pumps.7, Room δ1.5mm steel and steel body is made of.8, dust chamber body is made of EPS board, has entered out.9, water curtain spray booth technical parametersNo Item ParametersA flow rate (m3 / h) 90002 Power 43 pump power 34 open wind speed 0.6-0.75 Compressed air consumption (m3/min) 0.36 lamp illumination 400Z-7 nozzle8 Painting Room: Dimensions 2500 × 2400 × 26009 Dust Room: Dimensions 2500 × 1800 × 3400Note: Manual spray system uses 你好gh-quality brand-name products in Taiwan.6, leveling RoomLeveling effect of room to spray paint surface after the paint on the share level, and to a number of solvent evaporation in order to prevent baking too quickly appeared in the film pinhole.1, Room body is made of welded steel and steel plate.2, chamber body wall windows with transparent glass observation and maintenance of the door.3, chamber body with the top emission device, keeping indoor air fresh.4, room spray processing site in vivo, uniform color, elegant appearance.3, the consolidated statementa, baking oven is designed to bridge Road.

没有碰到水帘的漆雾由水帘下部进入喷漆室的后部，由喷嘴喷出的水雾冲洗、迷宫折板过滤及折板上的喷淋系统三次过滤后，废气经风机排至大气中，布局特点如次：一、设置双工位水帘喷漆室。二、槽体采用型钢及Q235钢板制作，板厚4mm。水帘板为不锈钢板，喷淋捕捉喷嘴为雾化喷嘴，抽风机采用防爆型风机。3、室体拼装板之间采用螺栓连接、安装、拆卸、迅速，便捷。四、配置喷漆室专用防爆照明，内部采光充足、明亮。5、漆雾处理采用水帘、喷淋捕捉加迷宫折板，可高效去除漆雾。6、轮回泵采用占地少、安装简略的管道泵。7、室体采用δ1.5mm钢板与型钢制作而成。八、防尘室体采用EPS板制成，设有出进门。9、水帘喷漆室技术参数序号项目参数1 处理风量（m3/h） 90002 功率 43 水泵功率 34 开口风速 0.6-0.75 压缩空气耗量（m3/min） 0.36 照明灯照度 4007 喷嘴 Z型8 喷漆室：外形尺寸 2500×2400×26009 防尘室：外形尺寸 2500×1800×3400注：手动喷漆系统采用台湾优质名牌产品。六、流平室流平室的作用是使喷漆后喷在工件表面上的漆铺平，并使溶剂挥发一些，以防止烘烤过快而在漆膜上浮现针孔。一、室体采用型钢与钢板焊制而成。

And then adding NAA at low concentration in MS was used to give rise to roots. We also found that lower level of hormone could control effectively browninng and vitrifaction during the culture and G1 n (6mg/L) and AgNOj (2mg/L) supplemented in shoot induction media could improve the shoot information rates apparently (about90%).The whole period of plant regeneration from leaflets of peanut could be divided as five steps: germination - shoot induction -shoot elongation-rooting- tranplant.

用1／2MS培养基萌发花生种子，9-10d后，从无菌花生苗上切取幼嫩叶片中部为外植体。2500Lux光照和27±1℃条件下，在诱芽培养基(MS+BA3mg／L+NAA0.8mg／L+AgNO_32mg／L十Gln 6mg／L)培养12-14d即可观察到明显芽点或瘤状突起，较前人报道的培养时间大大缩短了，4w后芽点进一步发育成丛生芽，芽诱导率达90.2％，每个外植体平均产9个丛尘芽，然后转至培养基MS+BA3 mg／L+AgNO_32 mg／L上诱导芽的伸长，3-4w后可长至3-4cm，切下带有2-3片叶片的幼芽移至生根培养基(MS+NAA0.8mg／L+AgNO_32mg／L)，1w后切口处可见白色不定根形成。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Results Mucopolysaccharidosis typeⅠ had characteristic X-ray changes of bones:①Enlarged skull and hook- shape Sella Turcica;②Short and thick phalanges and metacarpal Bones;③Dilated central part and thin ends of upper and lower limb bones;④Kyphosis deformity of spine, beak-shape stick out of anteroinferior margin of Kyphosis deformity vertebra;⑤Broadening ribs as paddle shape;⑥Shallow acetabulum and enlarged Acetabular Angle;⑦Prolonged femoral neck, Coxa Vara or coxa valga.

结果 粘多糖病Ⅰ型具有较典型的骨骼X线改变，(1)头大，蝶鞍呈乙型；(2)掌指骨粗短；(3)上、下肢骨中央膨胀两端变细；(4)脊椎后凸成角，后凸椎体前下缘成鸟嘴状突出，上下椎体呈卵圆形；(5)肋骨增宽呈船桨；(6)髋臼变浅，髋臼角增大；(7)股骨颈增长，髋内翻或外翻。

Methods Respectively analyzed the X-ray, CT and image post-processing manifestations of mucopolysaccharidosis type Ⅰ. Results Mucopolysaccharidosis typeⅠ had characteristic X-ray changes of bones:①Enlarged skull and hook- shape Sella Turcica;②Short and thick phalanges and metacarpal Bones;③Dilated central part and thin ends of upper and lower limb bones;④Kyphosis deformity of spine, beak-shape stick out of anteroinferior margin of Kyphosis deformity vertebra;⑤Broadening ribs as paddle shape;⑥Shallow acetabulum and enlarged Acetabular Angle;⑦Prolonged femoral neck, Coxa Vara or coxa valga.

结果 粘多糖病Ⅰ型具有较典型的骨骼X线改变，(1)头大，蝶鞍呈乙型；(2)掌指骨粗短；(3)上、下肢骨中央膨胀两端变细；(4)脊椎后凸成角，后凸椎体前下缘成鸟嘴状突出，上下椎体呈卵圆形；(5)肋骨增宽呈船桨；(6)髋臼变浅，髋臼角增大；(7)股骨颈增长，髋内翻或外翻。

They are most abundant in quartz of pegmatitic leucosomes and granitic rocks, and represent the fluid appearing in their cooling stage. The DL(H2O) of such fluid is 0.93～0.96g/cm3 corresponding to P≈0.6GPa which is compatible with condition when the rapid decompression in this region was ended. 3 CO2-H2O two or three phases inclusions. They are the most widespread, and more frequently in clusters and in intragranular tails in quartz. The relative content of CO2+CH4 and H2O is considerably variable. They are also characterized with lower density (0.6～0.8g/cm3) and low pressure about 0.3～0.4GPa, and may be originated by mixture of carbonic liquid from breaking of most inclusions of peak stage and aqueous liquid of magmatic source. After entrapment, the further decrease of temperature to lower than 330℃ made such fluid separated to two or three phases.

主要集中于伟晶质脉体和花岗岩中，是这期岩浆冷凝过程析出的流体相当时就被封闭所成，密度为0.93～0.96g/cm3，相应压力约0.6GPa，这与峰期后迅速减压过程结束时的条件相符。3CO2-H2O两相和多相包体，分布最广，成簇状和拖尾状包体群，碳质和H2O相对含量很不均匀，整体密度相对最低，一般为0.6～0.8g/cm3，相应压力为0.3～0.4GPa，它们可能是大幅度减压过程中第一类包体大量爆裂析出的碳质流体与第二类H2O溶液流体在各处以不同比例混溶所成，它们被封闭后在降温至330℃以下时分裂成两相或三相包体。

RPC steel and RQ steel are constituted by bainite and martensite microstructures before tempering. The microstructure of RPC steel did not change obviously except some bainitic laths coalescing were detected by means of SEM during tempering, while the boundary of bainitic laths of RQ steel disappeared quickly and finally formed polygonal ferrite forms accompanied a dramatic decrease in hardness.

回火前两种钢板组织均为贝氏体和马氏体的复合组织，经RPC处理后的钢板随回火温度的提高组织没有明显变化，只是一些板条出现合并现象，而经过RQ处理后的钢板随回火温度的升高板条很快消失最终演变成多边形铁素体，硬度也随之大幅度下降。

Reefs, reef-mounds and lime mud mounds are all carbonate buildups, and the reefs and banks are important targets for petroleum exploration The reefs, banks and mounds should be divided from each other not only in theory, but also in exploration practice, because of so many differences in sedimentary compositions, fabrics, environments, distribution in time and space, and the characteristics of petroleum geology If the original reef or bank itself was fine reservoir rocks, then it would become a better reservoir after the reservoirconstructive diagenesis The formation of a high quality carbonate reservoir is controlled by two factors: the sedimentary microfacies and the reservoirconstructive diagenesis The first is the basis for the formation of a high quality reservoir, and with the high energy reefbank facies as the best; the latter includes the cataclasis, the dolomitization and the karstification The dolomitization and karstification are the best for the formation of a high quality reservoir The karstification has the widest distribution, profoundest effect, and plays a vital role in the high quality reservoir forming The karst types are quite rich in the limestone reef or bank reservoir, including synsedimentary karst, penesedimentary karst, supergene karst and hydrothermal karst, and so does the dolomitization reef or bank reservoir The formation mechanism of synsedimentary karst and penesedimentary karst is related to the short time exposing and leaching of the cyclical highfrequency shallowupward sequence when the reefs or banks are forming The supergene karst is controlled by the exposing and leaching of tertiary cyclic sequence type I interface after the reefs or banks forming Thus, there developed a great deal of secondary corrosion pores, corrosion fractures and huge caves besides some original pores remained in the reef and bank reservoir Scarcely there are any original effective pores in reefmounds and lime mud mounds, especially in senkes among mounds, but secondary pores could be formed by karstification, and there would likely develop source rocks with high organic abundance

礁、礁丘、丘同属碳酸盐建隆，礁、滩是重要勘探目的层。但各自在沉积组分、组构、环境和时空分布，以及油气地质条件上迥然不同，因而无论从理论还是勘探实践上，都有必要将其各成体系划分开来。礁滩自身为好储集岩，当建设性成岩作用叠加后可形成好的储层。碳酸盐岩优质储层形成，主要受沉积微相与建设性成岩作用两大因素控制。前者是基础，并以高能的礁滩相带为最好；后者主要包括破裂、白云石化和溶蚀三大类，并以白云石化或溶蚀为最优，其中的溶蚀分布最广泛、影响最深刻而对优质储层形成起决定性作用。礁滩储层中的古岩溶类型最为丰富多彩，包括同生期、准同生期、表生期和埋藏期热流体岩溶的所有类型，白云岩化礁滩体也不例外。其中，同生准同生期岩溶的形成机制与礁滩沉积时高频层序旋回向上变浅序列的短暂暴露淋溶有关，表生期岩溶则受控于礁滩沉积后三级层序旋回Ⅰ型界面的暴露淋溶。由此，导致礁滩储层中除了可以也是唯一保存原生孔隙外，还发育了次生溶蚀孔洞缝和大型溶洞。礁丘、灰泥丘尤其是丘间洼地中发育高有机质丰度烃源岩；它们自身没有有效的原生孔隙，但岩溶作用可以形成次生孔隙。

By removed ovary culture after positive and negative hybridization, produced interspecific hybridization, and have improved the hybrid seed through removed ovary culture and induced to shoot by culture medium MS, and produced hybrid F1, the plant of Brassica junce×B.pekinesis, the advantage characteristic of hybrid plant is similar to female nature;By cytology authenticating, chromosome number of F1 young flower bud cell (2n=28), it is the sum of monoploid chromosome number of female parent Brassica junce(2n=36) and male parent B.pekinesis(2n=20); The hybridization to Brassica junce×B.pekinesis, and embryonic bud come from removed ovary culture, and it found another form of plant embryo saving.

以芸薹属植物芥菜型油菜和白菜为研究对象，对其进行常规正反杂交后子房的离体培养，通过离体子房培养获得的杂种种子经MS培养基诱导成苗，获得了芥菜型油菜与白菜的杂种F1代植株，表现出杂种优势；通过细胞学的方法，鉴定了杂种F1幼蕾体的细胞染色体数为2n=28，为母本芥菜型油菜（2n=36）和父本白菜（2n=20）的单倍体染色体数之和；芥菜型油菜与白菜常规杂交后，经离体子房培养获得了&胚性芽&，确立了植物胚挽救的另一种形式。

Several useful computer codes for analysing 2—D and 3—D transonic flow past bodies of revolution, fuselage configurations and highly swept wings have been developed in different types of grid topologies according to Holst's highly efficient AF2 algorithm of finite difference method.

九、研制了一整套在不同网格拓扑上用于分析旋成体、任意截面机身、以及大后掠细长机翼跨音速气动特性的实用程序。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。