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Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P<0.001.

结果:构建了带有HSV-tk基因的反转录病毒载体GINaTK,应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317,成为产病毒细胞PA317TK,用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞,命名为C6TK细胞,对GCV和ACV的敏感性分别高于亲本450倍和10倍;成功地建立了大鼠脑胶质瘤模型,并证实转染细胞C6TK的成瘤效应未改变,存活期约为15天;而经ACV治疗后,含有C6TK细胞的肿瘤生长明显被抑制,大鼠生存期延长为57.8±8.07天,尤其是采用PA317TK细胞混和治疗组和原位治疗组,肿瘤基本消失,大鼠生存期显著延长,混和治疗组存活120天以上,原位治疗组存活至71.4±36.1天。t检验,P值均小于0.001。

Results: GINaTK retroviral vector containing HSV-tk was constructed and transduced into PA317 retrovirus packaging cell by lipofectin (named PA317TK). Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P <0.001.

结果:构建了带有HSV-tk基因的反转录病毒载体GINaTK,应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317,成为产病毒细胞PA317TK,用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞,命名为C6TK细胞,对GCV和ACV的敏感性分别高于亲本450倍和10倍;成功地建立了大鼠脑胶质瘤模型,并证实转染细胞C6TK的成瘤效应未改变,存活期约为15天;而经ACV治疗后,含有C6TK细胞的肿瘤生长明显被抑制,大鼠生存期延长为57.8±8.07天,尤其是采用PA317TK细胞混和治疗组和原位治疗组,肿瘤基本消失,大鼠生存期显著延长,混和治疗组存活120天以上,原位治疗组存活至71.4±36.1天。t检验,P值均小于0.001。

They detected 112 sentinel lymph nodes in 71 patients (97%), including 64 (88%) in whom lymph nodes were stained blue after intraparenchmal injection of isosulfan blue, and 61 (84%) in whom lymph nodes were radioactive, reflecting uptake of radiocolloid injected intradermally.

研究者检测71位患者(97%)的112个前哨淋巴结,其中有64位患者(88%)的患者软组织注射isosulfan blue后,其淋巴结被染成蓝色,61位患者(84%)的淋巴结有放射活性,这反映皮内注射的放射胶体被吸收。

Based on the summarizing of the exiting algorithms of the texture mapping and antialiasing, this paper goes deeply into the basic theory and processing of texture mapping. The texture compress algorithm which can satisfy the need of the memory and time is given for the stochastic sampling in the inverse texture mapping; the geometry transformation in the texture mapping are discussed and the concepts of the unitary parametrization and the patch parametrization is proposed, some kinds of the unitary parametrization methods of parametric surfaces and the geometry transformation in the patch parametrization are also presented; in order to solve the aliasing in the parametrization of intermediate surface-sphere in two-part texture mapping, an area-preserving transformation is presented also; to improve the quality of the graph the aliasing in the texture mapping is analyzed from the aspect of signal process, and the modified summed-area tables algorithms is proposed; the distortion of the scanline conversion algorithms under perspective transformation is analyzed and the proper interpolation algorithm is presented so the aliasing problem of the polyhedral object after the unitary parametrization is efficiently solved.

本文在总结现有的纹理映射和反走样算法的基础上,深入研究了纹理映射的基本原理与过程,根据反向纹理映射对纹理随机采样的特殊要求,提出了一种可满足存储空间和寻址时间的纹理压缩存储算法;通过对纹理映射中几何变换理论的进一步深入探讨,提出了整体参数化和面片参数化的概念,并给出了参数化曲面的各种整体参数化方法和面片参数化的几何变换形式;针对现有的两步映射算法所存在的中间曲面—球面的参数化问题,提出一种等积映射方法,较好地实现了由非参数化面片拼接而成的多面体表面的纹理映射;从信号采样的角度深入分析了纹理映射中走样产生的原因,提出了改进的区域求和表算法,使图形生成的效果有所提高;还分析了一般的扫描线算法在透视变换中所存在的问题,提出了一种正确的插值算法,从而解决了整体参数化后多面体表面纹理映射的几何变形问题。

Under different spraying electric, the microhardness and the porosity of the coating are in inverse proportion relationship.

不同喷涂电流下,涂层的显微硬度与涂层孔隙率成反比例关系,而孔隙率又是随电流的增大先增加后减小的。

The huge pool of blood in front of her was already assuming the iridescence of coagulation; and when the sun rose a hundred prismatic hues were reflected from it.

她面前的一大滩血已经凝结成彩虹般的一道;太阳升起后,照在上面反射出缤纷的色彩。

The microstructure and compositions of the spots on the blue colours of Jingdezhen Blue andWhite porcelain were investigated. The spots on the blue colours of Blue and White porcelainare the result of crystallization and enrichment of manganese and iron contained in cobalt bluepigment after high temperature sintering, and the compositions of the spots mainly depend on thecontents of manganese and iron in cobalt blue pigment.

通过对元代、明洪武及明宜德年间的景德镇青花瓷样品彩上斑点的显微结构和组成分析,研究了斑点的形成机理和组成,表明青花瓷彩上斑点是由青花钴料中的锰、铁、钴,经高温过程后,在釉熔体中的偏析所形成的,其物像组成,除主要取决于青花钴料中的锰、铁含量外,样品的胎、釉组成及烧成机制等也影响着铁的价态。

Men with depression are more likely to report fatigue, irritability, and loss of interest in work.

男人患上抑郁症后很可能将它说成疲劳、易激动和对工作失去兴趣。

Isidore of Seville, is in Latin, called 'unctio' and from it Christ receives His name, and man is sanctified after the laver ; for as in baptism remission of sins is given, so by anointing the sanctification of the Spirit is conferred.

" chrisma "说,圣伊西多尔的塞维利亚,是在拉丁美洲,所谓' unctio ',并从基督得到他的名字,而人是神圣化后,紫菜;如同洗礼减免的罪孽是由于,所以由anointing 成圣的精神,是赋予。

Methods: Polytetrafluoroethylene and polypropylene film electrets were prepared by constant voltage corona charging at room temperatures. The charge-store stability of PTFE and PP were studied by measuring isothermal surface potential decay after different dose of ultraviolet radiation.

用常温恒压电晕充电系统将聚四氟乙烯和聚丙烯两种材料极化制备成驻极体;用补偿法测量不同剂量的紫外照射后驻极体的表面电势。

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