后成

After washed, the chromogen substrate was added and incubated for 30 minutes in the dark. Rinsed with distilled water, purple spots were observed and counted using a dissection microscope. The number of spots means the level of cytokine producted by sCTL. Analysis of ability of specific cytotoxic lymphocytes to lyse target cells: CytoTox96 Non-Radioactive Cytotoxicity Assay kit was used.

5特异性CTL裂解靶细胞能力测定：采用CytoTox96 Non-RadioactiveCytotoxicity Assay试剂盒，将经HBV core18-27短肽、重组乙肝病毒核心抗原共刺激培养13日的PBMC与HLA-A2〓的靶细胞按一定比例混合，靶细胞被裂解后，释放的乳酸脱氢酶与底物反应显色，酶标仪测定吸光度，吸光度与裂解程度成正比，按照公式计算百分裂解率。

He became a chronicler of contemporary history in 1834, when he produced stunning images of the burning of the houses of parliament.

当透纳1834年完成了这幅描绘国会大厦失火事件的大作后，他成了那个年代历史的记录者。

Also the molecular structure and functional effects of this active component were studied in detail. 1 Extraction, separation, purification and structural identification of the functional component in Chufa After initial handling of waste slurry to solid powder, ethanol was used to lixiviate it followed by the extraction with petroleum ether,ethyl acetate,n-butyl alcohol one by one, the polarity of three organic reagents being changed from low to high.

荸荠功能因子的提取、分离、纯化及结构鉴定以马蹄粉厂产粉废浆为原料，经过实验室初处理成固态原料粉后，用乙醇浸提，再将浸提物以水悬浮，依次通过极性从小到大的石油醚、乙酸乙酯、正丁醇三种有机溶剂两相萃取分部，并以金黄葡萄球菌、枯草芽孢杆菌和致病大肠杆菌为指示菌，检测上述各部分提取物的功能效果。

The results demonstrated that the measured molecular weight of phycocyanin was 37.715Kd, and consisted of two peptides which were similar with their molecular weight and amino acid sequences.4 The comformation of phycocyaninPhycocyanin high structure was investigated, as the result of circular dichroism spectrum and Fourier transformation infared spectrum shown, the secondary structure of NSPC was mainlyα-helix structure and the content ofβ-shee or coil was very small.

根据氨基酸自动分析仪测定的氨基酸组成和精确分子质量计算出NSPC分子总共有331个氨基酸残基，分别是由40个Asp、22个Thr、18个Ser、22个Glu、1个Gly、31个Ala、27个Cys、17个Val、13个Ile、22个Leu、5个Tyr、7个Phe、7个Lys、24个His、51个Arg、14个Pro、1个Met和9个Trp组成。胰蛋白水解酶分别酶解NSPC-1和NSPC-2后，分别采用MALDI-TOF-MS分析，其肽指纹谱图显示胰蛋白酶可将NSPC-1和NSPC-2水解成大小不等的多条肽段，质量数在800以上的NSPC-2有11段，即水解的肽质量分别为899.54、1454.77、1544.9、1683.87、1803.93、1811.91、1987.92、2003.90、2536.26、2915.42和3492.72。

After Taro Aso's Liberal Democratic Party was humiliated in Tokyo's municipal elections—the ruling coalition lost its majority in the city's Assembly and the opposition Democratic Party of Japan became the largest party—Japan's prime minister called an early election for August 30th.

日本首相麻生太郎领导的自由民主党在东京的市政选举中受到羞辱后，这个现任的统治联盟失去市议会的大多数选票，反对党日本民主党成了最大的政党，日本首相号召8月30日进行早期的大选。

Neither of them are big rivers, but very large when joined together, and yet larger when they are joined by a third river, which joins them near Clarendon Park, about three miles below the city.

这两条河都不大，但这两条河汇合以后则是一条很大的河，而且当它与在该市以下3英里克拉伦登公园处的第三条河汇合后则成了一条更大的河流。

Under this condition, the percent of the BaO in the clinker aggregate is up to 54%, which is above 70% according to the rate of conversion.

在这种条件下，毒重石焙烧后的熟料中BaO的含量可达54%，换算成转化率即为70%以上。

During the peak stage of the deformation under high stress environment, mass medium fracture and sticky slip will appear in cracks, and they are represented as solid tides anomalies in the records of clinometer.

大地震在主破裂前往往行成地壳变形，高应力作用下进入峰值后的变形阶段，常有大量介质破裂及裂隙间的粘滑产生，反映到地倾斜记录仪上，表现为固体潮形变异常。

Methods 5 postlingually adults,3 males and 2 females,participated in the test.The MACC Battey was used as test material.The test included both close-set phonetic recognition and open-set word and sentence recognition.

5例语后聋成人中，应用MACC测试材料，分别进行了包括封闭项列(机会水平0.25)和开放项列两大部分的言语识别测试。

After this companys product is and the valve assembles the electrically operated valve or the air operated valve uses, they use for to control the valve opening or the closure, the operator may in the scene manual operation, perhaps the long-distance range carry on the control in the control room.

本公司的产品是和阀门组装成电动阀门或气动阀门后使用的，它们用来控制阀门的开启或关闭，操作人员可以在现场手动操作、或是在控制室内远距离进行控制。同时还提供与各种型号匹配的控制器、控制箱等。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。