后成

Use constituent piece to stick mural way depart to foster Niu Er to become fiber cell above all, after classics generation is rarefied, fat simple way will be contained double the skin of the 4th acting ox that outside conveying carrier to turn into body, the particularity of person bacteriolysis enzymatic mammary gland that chooses number fosters becomes fiber cell, g/mLG418 chooses 500 μ 2 weeks, g418 halve continues to develop 3 generation of 2 ～, catch the cell after choosing to undertake PCR detects to turning next reach nucleus analysis.

首先采用组织块贴壁法分离培养牛耳成纤维细胞，经传代纯化后，脂质法将含有双重筛选标记的人溶菌酶乳腺特异性表达载体转入体外培养的第4代牛皮肤成纤维细胞，500μg/mLG418筛选2周，G418减半继续培养2～3代，然后对转染筛选后的细胞进行PCR检测及核型分析。

Used in shaping process of brick production , the treated clay will be plastic extruded by up stage then via vacuum room into down stage, estruding into solid or hollow clay column of required cross section and shape which then cut into greenbrecks by green breck cutter.

JKR40/40型双级真空挤砖机适用于砖瓦生产成型工段，可将经过处理的原料经过上级增塑挤出后落入真空室抽取空气后再经过下级挤成规定断面的实心或空心坯条，再经过切坯机制成一定规格的砖坯。

Results: Fifty-two of 66 patients with unilateral fractures were, after 9–13 years, rated as excellent/good according to the Green and O'Brien score as modified by Cooney et al.

结果：66例单侧桡骨无故骨折患者，有52例在伤后9－13年GOBC评分为优良，AO骨折分类与结果无关，相当多的患者仍遗留骨折移位：背侧成角（小于60岁者，平均13°，大于60岁者，平均18°），与伤时相比桡骨存在短缩（小于60岁者，平均2mm，大于60岁者，平均3mm），5例患者复位后仍存关节面缺损（1－2mm），但是仅有1例患者后来发展成了轻度的骨性关节炎。

Curtain plate stainless steel plate, spray nozzle for the spray nozzle to capture, exhaust fan with explosion-proof fan.3, chamber body assembly plate connection between the use of bolts, installation, removal, fast and convenient.4, configuration, special explosion-proof spray room lighting, interior lighting sufficient light.5 mist treatment with water curtain, shower folding capture plus maze can efficiently remove the paint mist.6, circulation pump with an area of small, simple installation and pipeline pumps.7, Room δ1.5mm steel and steel body is made of.8, dust chamber body is made of EPS board, has entered out.9, water curtain spray booth technical parametersNo Item ParametersA flow rate (m3 / h) 90002 Power 43 pump power 34 open wind speed 0.6-0.75 Compressed air consumption (m3/min) 0.36 lamp illumination 400Z-7 nozzle8 Painting Room: Dimensions 2500 × 2400 × 26009 Dust Room: Dimensions 2500 × 1800 × 3400Note: Manual spray system uses 你好gh-quality brand-name products in Taiwan.6, leveling RoomLeveling effect of room to spray paint surface after the paint on the share level, and to a number of solvent evaporation in order to prevent baking too quickly appeared in the film pinhole.1, Room body is made of welded steel and steel plate.2, chamber body wall windows with transparent glass observation and maintenance of the door.3, chamber body with the top emission device, keeping indoor air fresh.4, room spray processing site in vivo, uniform color, elegant appearance.3, the consolidated statementa, baking oven is designed to bridge Road.

没有碰到水帘的漆雾由水帘下部进入喷漆室的后部，由喷嘴喷出的水雾冲洗、迷宫折板过滤及折板上的喷淋系统三次过滤后，废气经风机排至大气中，布局特点如次：一、设置双工位水帘喷漆室。二、槽体采用型钢及Q235钢板制作，板厚4mm。水帘板为不锈钢板，喷淋捕捉喷嘴为雾化喷嘴，抽风机采用防爆型风机。3、室体拼装板之间采用螺栓连接、安装、拆卸、迅速，便捷。四、配置喷漆室专用防爆照明，内部采光充足、明亮。5、漆雾处理采用水帘、喷淋捕捉加迷宫折板，可高效去除漆雾。6、轮回泵采用占地少、安装简略的管道泵。7、室体采用δ1.5mm钢板与型钢制作而成。八、防尘室体采用EPS板制成，设有出进门。9、水帘喷漆室技术参数序号项目参数1 处理风量（m3/h） 90002 功率 43 水泵功率 34 开口风速 0.6-0.75 压缩空气耗量（m3/min） 0.36 照明灯照度 4007 喷嘴 Z型8 喷漆室：外形尺寸 2500×2400×26009 防尘室：外形尺寸 2500×1800×3400注：手动喷漆系统采用台湾优质名牌产品。六、流平室流平室的作用是使喷漆后喷在工件表面上的漆铺平，并使溶剂挥发一些，以防止烘烤过快而在漆膜上浮现针孔。一、室体采用型钢与钢板焊制而成。

But in SD rats, cardiac muscle fibers were fractured seriouly , contracted to wavilness obviously, then muscle fibers fracture were contracted like entasia by the hypoxia time increased , injury intensify , all the changes refered SD rats compensatory mechanism can not be transferred in this hypoxia degree. 3. Exposed to 4% oxygen concentration, the cardiac muscle ultrastructure of Gansu zokors had serious injury after hypoxia 2h.

在4%氧浓度的低氧耐受实验中，甘肃鼢鼠在低氧2，4h后，心肌纤维收缩明显呈波浪状，低氧时间延长至6，8和10h后，心肌显微波浪状收缩由强逐渐变弱，并逐渐恢复至正常状态，说明鼢鼠心肌在6h以后对严重缺氧环境开始代偿性适应；SD大鼠2h组心肌纤维断裂严重，收缩加强成明显的波浪状，随着低氧时间得增加，4h组呈严重成挛状收缩，损伤加剧，提示SD大鼠在这一氧浓度下已经无法正常调动其代偿机制。3。

After the analysis it was concluded that the mean velocity fluctuations of the strata under Jidike Group in this working area could be divided into three zones: the minor velocity fluctuation zone in southern Dalaoba tectonic belt, the steep velocity fluctuation zone of the foreslope in middle YK structure and the zone from the bowtype thrusting tectonic belt in northern Qiulige to the minor velocity fluctuation zone in YK tectonic zone. On the basis of a good command of the regular patterns of the velocities in vertical and horizontal dimensions in the investigating area, the stack velocity was interpreted in a interactive mode by using the Depthteam Express modelling package of the Landmark system, converting the stack velocity into mean velocity by means of Dix formula. Based on the well drilling layering data, the velocity correction was executied to get the final corrected velocity model by using the Kriging Correction. By using this velocity model, we did the time to depth conversion and obtained the final depth structural map. After mapping by using the variable velocities, we came in for the display of the tectonic map of the seismic data.

经研究认为本区的吉迪克组以下层位平均速度变化可分成三个变化区域：南部大涝坝构造带速度变化平缓区；中部亚肯构造前缘斜坡区速度变化强烈区；北部秋里塔格弧形逆冲构造带前缘至亚肯构造带速度变化平缓区，在撑握工区速度纵、横向变化规律基础上，应用Landmark解释系统Depthteam Express速度建模软件包对迭加速度进行交互解释，利用DIX公式把迭加速度转换成平均速度，根据区内钻井分层数据采用克里金校正方法进行速度校正，得到最终校正后的速度模型，用此速度模型进行时深转换，得到最终的深度构造图，此次变速成图后，得到地震资料构造图显示。

Look from the content that informs against a letter, the person that inform against says is " blue sky lanneret " child, they and " blue sky lanneret " get acquainted with in sina UC chatting room, over,"Blue sky lanneret " impart to them fry a skill, after paying 10 thousand yuan of tuition each, became " blue sky lanneret "" close door child ", learn with him " fry a stunt ", formal courses by a definite date 2 weeks, after learning, often also can consult.

从举报信的内容看，举报者都称是"蓝天雄鹰"的弟子，他们与"蓝天雄鹰"在新浪UC聊天室中结识，在那里，"蓝天雄鹰"向他们传授炒股技巧，每人在交了1万元学费后，成了"蓝天雄鹰"的"关门弟子"，跟他学习"炒股绝技"，正式课程为期2周，学成后也可以不时请教。

A self-drilling anchor (10) for use in a friable material (1) comprises a body (12) having an axis (13), a flanged rear end (14), a drilling front end (16) and a generally cylindrical portion (18) therebetween having an outer surface (20) with a thread (22) disposed thereon, wherein the body forks, beginning at a predetermined distance from the flanged rear end, into a first leg (24) and a second leg (26), the first leg extending forwardly into a drilling tip (28) and having a generally rearward facing shoulder (30) angled obtusely outwardly with respect to the axis, wherein the body (12) has an axial bore (32) for receiving an elongate fastener (2), the axial bore (32) extending substantially through the flanged end (14) and the generally cylindrical portion (18) and leading to the generally rearward facing shoulder (30), wherein the anchor (10) has a drilling mode wherein the second leg (26) nests behind the generally rearward facing shoulder (30) of the first leg (24), and an anchoring mode wherein the legs are pivoted apart from one another.

一种用于易碎材料(1)中的自攻螺钉(10)，包括：主体(12)，其具有中心轴(13)、带有凸缘的后端部(14)、用于钻孔的前端部(16)和位于所述后端部和前端部之间的通常为圆柱状的部分(18)，所述圆柱状部分(18)的外表面(20)上布置有螺纹(22)，其中主体从距离带有凸缘的后端部的预定的距离处开始分叉为第一腿(24)和第二腿(26)，第一腿向前伸入钻孔尖端(28)，并且具有相对于中心轴向外成钝角的通常面向后的肩部(30)，主体(12)具有容纳细长状紧固件(2)的轴向孔(32)，轴向孔(32)基本延伸穿过带有凸缘的端部(14)和通常为圆柱状的部分(18)，并且通向通常面向后的肩部(30)，螺钉(10)具有钻孔状态和固定状态，在钻孔状态，第二腿(26)套在第一腿(24)的通常面向后的肩部(30)之后，并且在固定状态，腿彼此转动分离。

PartⅡThe mechanism elucidation about effects ofα-(2,3)/(2,6)sialic acid on the Cx43gap-junction functions.(1) Westernblotting experiment showed that the decrease of sialic acid didn\'t changethe Cx43 expression and its phospholation level.(2) Westernblotting experiment showed sialidase didn,t change the ZO-1 expression,IP and confocal experiment showed sialidase improved the interaction of Cx43 andZO-1.(3) Westernblotting experiment showed sialidase didn\'t change N-cadherin expression,IP and confocal experiment showed sialidase promoted the complex formation ofCx43 and N-Cadherin.(4)Sialidase could increase the ERK1/2 phospholation level,and enchanedintercellular homotypic adhesion,Immunofluorometric assay showed sialidase couldpromote the N-cadherin cluster on the membrance.

第二部分：α—(2，3)／(2，6)唾液酸对肿瘤细胞CX43间隙连接功能影响的机制研究1、Westernblotting结果表明降低细胞膜表面唾液酸并不改变Cx43的表达及其磷酸化水平：但Cx43连接斑形成增多；2、Westernblotting结果表明唾液酸酶作用后细胞ZO—1表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与ZO-1的结合；3、Westernblotting结果表明唾液酸酶作用后细胞N-cadherin表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与N-Cadherin复合物的形成；4、唾液酸酶作用后细胞内ERK1／2磷酸化水平明显增加，细胞间同质粘附增加，以及免疫荧光表明唾液酸降低后可促进N-cadherin的膜成簇。

To understand the infectivity by porcine endogenous retrovirus with porcine skin fibroblast cell in vitro and in vivo, porcine skin fibroblast cell established by our laboratory were co-cultured with neo/HEK293 cell for the infection of RERV in vitro, and were subcutaneously transplantated to SCID (severe combined immuno-deficiency) mice for the infection of PERV in vivo, laying the foundation for valuation of biologic safety of xenotrans-plantation. The event of neo/HEK293 cells infected by PERV occurred during co-culture of porcine skin fibroblast cells with neo/HEK293 cells, expanding the rang of the infection of porcine endogenous retrovirus. Afterpig cells transplantated subcutaneously in SCID mice, the microchimerism (78.57%) of pig cells occurred widel, and there was phenomena of integration of PERV provirus (85.71%) in several organs or tissues remote from the injected sites, indicating infection of PERV in SCID mice in vivo. yet, there is no evidence of active viral replication in analysis of PERV env RNA of these tissues or organs.

为了解猪皮肤成纤维细胞PERV在体外和体内的感染性，通过建立猪皮肤成纤维细胞系，将所建细胞系与人胚胎肾293细胞体外共培养，并移植于严重联合免疫缺陷鼠皮下进行猪皮肤成纤维细胞PERV的体外和体内感染性实验，结果表明，猪皮肤成纤维细胞与人胚胎肾细胞共培养过程中，猪内源性逆转录病霉感染人胚胎肾细胞，进一步证实和拓宽了猪细胞PERV感染人细胞的范畴；猪皮肤成纤维细胞移植SCID鼠皮下后，导致SCID鼠发生猪细胞微嵌合(78.57%)和PERV在体内感染(85.71%)并且波及远离移植部位的多种组织或器官，但是并未检测出SCID鼠组织中表达PERV env RNA。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。