后成

4DRG was co-culture with sciatic nerve segment in 10鸖 DMEM;the axons were longer and surround the sciatic nerve segment which was regard as anew evidence for chemotropism.

结果：(1)在无血清条件下单独培养的DRG，背根神经节的轴突数目众多，外形纤细弯曲，不成束，并且施万细胞和成纤维细胞稀少，所以可以排除两者对轴突生长的影响，为观察来源于变性坐骨神经段的可溶性因子对轴突生长的作用提供了有利条件；(2)在无血清条件下DRG和变性坐骨神经段联合培养，①先单独培养DRG，4天后待神经元轴突长出，再与坐骨神经段联合培养，观察到神经元的轴突数目减少，外形挺直，部分轴突之间相互粘附成束；②变性坐骨神经段和DRG同时联合培养，神经元的轴突数目明显减少，外形粗壮，轴突之间相互粘附成束；(3)有血清条件下单独培养DRG，轴突数目较多，外形挺直，长短不一，部分神经元的轴突之间相互粘附，施万细胞和成纤维细胞数目众多，观察到的轴突生长情况受到施万细胞和成纤维细胞的直接或者间接的影响。

The material for formation of the granitoids isderived from mixing sources of crust and mantle, with decreasing in crust-derived componentsfrom early to late.5. The tectonic setting for formation of the Mesozoic granitoids in the region isdefined as a post-collisional setting. The occurrence of a small part of rocks with characteristics ofcollisional or anorogenic granitoids provides evidence for the continuous process oftransformation of tectonic regime from collisional through post-collisional to anorogenic.6. Themultiple cycles of mantle-derived magma underplating caused by the multiple stages oflithospheric thinning in the Mesozoic result in the partial melting of middle to lower crust orcrust-mantle syntexis to form magmas. The crystallization of these magmas emplacing afterfractionation is responsible for the formation of the Mesozoic granitoids in this region.

形成花岗岩类的成岩物质来自壳幔混源。5、河南省西部地区中生代花岗岩类成岩构造环境为碰撞后环境，部分岩石具有碰撞期或非造山期花岗岩的某些特征，表明碰撞后阶段既是碰撞造山过程的延续，又是向非造山环境的构造转换时期，因而其岩浆活动在一定程度上仍然继承了碰撞前消减时期和碰撞时期已经活化的岩浆源区性质。6、研究表明，区内中生代多阶段的岩石圈减薄作用引发多旋回的幔源岩浆底侵以及中下地壳的部分熔融或壳幔同熔，由此形成的岩浆经分异演化后侵位可以形成区内的中生代花岗岩类。

The invention provides a ginkgo pyroligneous liquor and the application thereof in promoting the seed germination of crops and the growth of seedling, and the ginkgo pyroligneous liquor is prepared according to the following steps: after ginkgo wood is split into wood chips, the charring of ginkgo wood is carried out in a retort, the gas generated in the process of charring is condensed, the liquid after condensation is collected and then is placed still for three months, after that, supernatant liquid is separated, thus obtaining the ginkgo pyroligneous liquor.

本发明提供一种银杏木醋液及其在促进作物种子发芽和幼苗生长中的应用，所述银杏木醋液按如下步骤制备而成：将银杏木材劈成木片后，在干馏釜中进行银杏木材的炭化，冷凝炭化过程中产生的气体，收集冷凝后的液体，将收集到的液体静置3个月后分离出上层液体，得到银杏木醋液。

RESULTS: After differentiation of human adherent myoblasts by neural induction medium, no cells with a neural cell morphology (ie., small, refractile cell body with dendritic cell extensions) were seen. All remaining myoblasts cultured with neural induction medium, myoblasts with proliferation medium and myotubes with differentiation medium containing 20 mL/L HS were positive for β Tubulin Ⅲ. C2C12 myoblasts were negative for β Tubulin Ⅲ. In contrast, all the above cells were negative for the markers Neurofilament Mr 68×103 and GFAP.

结果：用诱导分化液作用后，未见小的、伴有突起的放射状形态的神经细胞；抗β Tubulin Ⅲ对经神经元胶质细胞诱导分化液作用后的人成肌细胞、增殖培养液培养后的各代人成肌细胞及仅含20 mL/L HS分化液分化形成的肌管细胞染色均为阳性； C2C12细胞β Tubulin Ⅲ抗体染色阴性；上述所有细胞抗Neurofilament Mr 68×103和抗GFAP染色均为阴性。

Shim current calculation When shim coils are excited by appropriate current, they can generate correcting magnetic field to compensate the imaging magnetic field unhomogeneity. Based on the indirect calculation method, we proposed a novel iterative optimization algorithm to calculate shim current. Because there is a direct proportion relationship between FID signal and magnetic field homogeneity, we use the intensity of FID signal as feedback information to the calculation. Shim current can be worked out in less than 3 minutes. This satisfied the requirement that active shimming should be performed in real time.

5匀场电流计算对匀场线圈施加适当的电流后才能产生校正磁场消除成像磁场的非均匀分量，本文在匀场电流间接计算方法的基础上，利用磁共振成像原理中磁场均匀性与系统FID信号强度成正比的关系提出了一种电流迭代优化算法，将系统FID信号强度的变化作为反馈信息，能够快速计算出匀场电流对成像磁场进行补偿校正，平均耗时不到三分钟，满足磁共振成像系统对有源匀场的实时性要求。

Shim current calculationWhen shim coils are excited by appropriate current, they can generate correcting magnetic field to compensate the imaging magnetic field unhomogeneity. Based on the indirect calculation method, we proposed a novel iterative optimization algorithm to calculate shim current. Because there is a direct proportion relationship between FID signal and magnetic field homogeneity, we use the intensity of FID signal as feedback information to the calculation. Shim current can be worked out in less than 3 minutes. This satisfied the requirement that active shimming should be performed in real time.

5匀场电流计算对匀场线圈施加适当的电流后才能产生校正磁场消除成像磁场的非均匀分量，本文在匀场电流间接计算方法的基础上，利用磁共振成像原理中磁场均匀性与系统FID信号强度成正比的关系提出了一种电流迭代优化算法，将系统FID信号强度的变化作为反馈信息，能够快速计算出匀场电流对成像磁场进行补偿校正，平均耗时不到三分钟，满足磁共振成像系统对有源匀场的实时性要求。

The fibrils of three levels,Which were macrofibrils of several tens micron in diameter,fibrils feW micron and microfibrils about 0.3 micron,were observed simultaneously with banded texture usingpolarized microscope.After annealing,fibrils in different levels relaxed to differentmorphology.Macrofibrils still oriented along shear direction,but fibrils relaxed to helical form,and microfibrils relaxed to waveform therefore the banded texture canbe seen.The smaller the diameter of fibrils was,the more easily the fibrils relaxed.Ori the other hand,the banded texture decoration of annealed unoriented filmsrevealed directly the distribution of directors within and out of domains and thevariation of directors on boundaries.

微纤分三个层次，即直径分别为数十微米的微纤束、数微米的微纤和0.3微米的亚微纤，退火后三个层次的微纤松弛成不同的形态，微纤束仍笔直沿剪切方向取向，微纤松弛成螺旋形，而亚微纤松弛成波浪形从而观察到条带织构：微纤直径越小越易松弛，另一方面，非取向态织构退火后由亚微纤形成的条带装饰结果直观地揭示了微区内外分子指向矢的分布和它们在边界上的变化。

Reefs, reef-mounds and lime mud mounds are all carbonate buildups, and the reefs and banks are important targets for petroleum exploration The reefs, banks and mounds should be divided from each other not only in theory, but also in exploration practice, because of so many differences in sedimentary compositions, fabrics, environments, distribution in time and space, and the characteristics of petroleum geology If the original reef or bank itself was fine reservoir rocks, then it would become a better reservoir after the reservoirconstructive diagenesis The formation of a high quality carbonate reservoir is controlled by two factors: the sedimentary microfacies and the reservoirconstructive diagenesis The first is the basis for the formation of a high quality reservoir, and with the high energy reefbank facies as the best; the latter includes the cataclasis, the dolomitization and the karstification The dolomitization and karstification are the best for the formation of a high quality reservoir The karstification has the widest distribution, profoundest effect, and plays a vital role in the high quality reservoir forming The karst types are quite rich in the limestone reef or bank reservoir, including synsedimentary karst, penesedimentary karst, supergene karst and hydrothermal karst, and so does the dolomitization reef or bank reservoir The formation mechanism of synsedimentary karst and penesedimentary karst is related to the short time exposing and leaching of the cyclical highfrequency shallowupward sequence when the reefs or banks are forming The supergene karst is controlled by the exposing and leaching of tertiary cyclic sequence type I interface after the reefs or banks forming Thus, there developed a great deal of secondary corrosion pores, corrosion fractures and huge caves besides some original pores remained in the reef and bank reservoir Scarcely there are any original effective pores in reefmounds and lime mud mounds, especially in senkes among mounds, but secondary pores could be formed by karstification, and there would likely develop source rocks with high organic abundance

礁、礁丘、丘同属碳酸盐建隆，礁、滩是重要勘探目的层。但各自在沉积组分、组构、环境和时空分布，以及油气地质条件上迥然不同，因而无论从理论还是勘探实践上，都有必要将其各成体系划分开来。礁滩自身为好储集岩，当建设性成岩作用叠加后可形成好的储层。碳酸盐岩优质储层形成，主要受沉积微相与建设性成岩作用两大因素控制。前者是基础，并以高能的礁滩相带为最好；后者主要包括破裂、白云石化和溶蚀三大类，并以白云石化或溶蚀为最优，其中的溶蚀分布最广泛、影响最深刻而对优质储层形成起决定性作用。礁滩储层中的古岩溶类型最为丰富多彩，包括同生期、准同生期、表生期和埋藏期热流体岩溶的所有类型，白云岩化礁滩体也不例外。其中，同生准同生期岩溶的形成机制与礁滩沉积时高频层序旋回向上变浅序列的短暂暴露淋溶有关，表生期岩溶则受控于礁滩沉积后三级层序旋回Ⅰ型界面的暴露淋溶。由此，导致礁滩储层中除了可以也是唯一保存原生孔隙外，还发育了次生溶蚀孔洞缝和大型溶洞。礁丘、灰泥丘尤其是丘间洼地中发育高有机质丰度烃源岩；它们自身没有有效的原生孔隙，但岩溶作用可以形成次生孔隙。

BMSCs were isolated, depurated, cultivanted, and identified,then incubated with the concentration of 25μg Fe per milliliter at 37℃in 5% CO2. The labeled cells were stained by Prussian blue/trypan blue,and observed under fluorescent microscope.2. The labeled cells of different density (1×104/ml,5×104/ml,1×105/ml,5×105/ml,1×106 /ml,5×106/ml)were imaged by MRI with T1WI, T2WI and T2*WI sequences;and the same density (5×104/ml,1×105/ml)labeled cells were imaged by T2*WI sequences at different time.Then the signal intensities were measured and statistically analyzed.3. The model of rabbit renal ischemia-reperfusion injury was set up and treated. Then BMSCs(5×105)were injected into 16 recipient rabbits(1abeled cells in 10,unlabeled cells in 6)from ear vein.MR images of kidneys were obtained respectively at the time points of 0,1,3,5, 8 days after transplantation and before transplantation. MR imaging findings were analyzed,which were correlated with histological findings.

实验方法1分离、纯化、培养、鉴定兔BMSCs并以SPIO以25μg Fe/ml培养液浓度标记，对标记后不同时间的细胞行普鲁士蓝染色和台盼蓝拒染后显微镜观察。2将不同细胞浓度标记细胞管(1×104/ml、5×104/ml、1×105/ml、5×105/ml、1×106/ml、5×106/ml)，以不同扫描序列T1WI，T2WI，T2*WI(GRE进行MR成像，再选择相同细胞浓度组(5×104/ml、1×105/ml)进行不同时相MR成像，并测量信号强度，进行统计学分析。3缺血再灌注肾损伤模型建立和处理，然后将标记和未标记细胞(5×105个)经耳缘静脉移植入家兔体内(共16只：注入标记细胞者10只，注入未标记细胞者6只)，两组均于注射前、注射后第0、1、3、5、8天应用MRI对移植细胞进行活体示踪并与肾脏组织切片对照，然后对收集的信号强度进行统计学分析。

Combined with water and then covered with fire stew, and pour out of steam, with a kitchen knife into filaments, bit by bit into the cage, and then pickled lumps of pressed wood, put it step by step, after the full release Sorghum followed by pole size of the cut-off into the mouth urn-shaped section, I urn on the top, coupled with a "pebble" pressed, the urn will be placed in the shade, add water daily, so that the leaves ferment inside the urn after the juice is leaking "Red Water" spillover.

然后再加水上盖用火焖，蒸熟后倒出来，用菜刀切成细丝，一点一点地放进笼中，再用木制的腌菜疙瘩压紧，一层一层放置，放足后用高粱杆跟着瓮口大小截成段状，放在瓮口，上面再加一块&鹅卵石&压紧，将瓮置于阴凉处，逐日加清水，使瓮内菜叶发酵后渗出原汁即&红水&外溢。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。