后成

By using electroporation transfects,the TGF〓expression vector were introduced into HL-60 cells.Thecells were allowed to grow for 1 day before beingsubjected to selection for the ability to grow in mediumcontaining 600ug/ml geneticin.Stable cell clones resistantto G418 sulfate were obtained after 2-3 WK growth withdrug-containing medium.Southern blot hybridization wascarried out.RNA slot have higher expression of TGF〓mRNA in HL-60 transfected.All results followed suggestedthat TGF〓 have suppressed the maliganant phenotype of HL-60 cells.

利用电击法将〓表达载体导入HL-60细胞中，经G418筛选培养20天后再行Southern杂交检测外源〓整合情况，利用RNA狭线杂交法证明了在转录水平上表达有所提高，进一步进行了流式细胞仪测定DNA含量，使细胞在〓期产生生长阻滞作用，细胞生长速度，在软琼脂中成集落数、在组织中的浸润性及在裸鼠中的成瘤性均发生变化，我们的实验结果表明TGF〓对HL-60细胞具有抑制恶性表型的作用。

However the co-administration of bromocriptine (10 mg/kg) and baclofen (1.0 mg/kg) can inhibit the withdrawal response. The results showed that the two receptors synergized and effectively inhibited the naloxone-induced withdrawal syndromes.

当无抑制作用剂量的溴隐亭(10mg/kg)和巴氯芬(1.0mg/kg)联合应用时能够明显抑制小鼠的戒断症状，说明此二受体在吗啡成瘾后戒断期间功能上具有协同作用，能够很好地抑制纳络酮诱导的成瘾小鼠跳跃症状。

With the development of embryo, Myf5 expression decreased gradually in somites in the anterior region, but remained strong in the newly formed somites; After 30 somites formed, MyoD expression decreased in the somites except the caudal somites. At the hatching stage, MyoD and Myf5 were expressed in head muscle cells and fin muscle cells. In the growing fish, Myf5 was expressed in the skeletal muscle and intestine, and in adult flounder, Myf5 was only expressed in muscle. In the growing fish and adult fish, MyoD was only expressed in muscle.

在胚胎发育早期，Myf5在近轴中胚层中表达，体节发生过程中，Myf5在体节中表达，MyoD基因最早在分节板的体节前细胞中表达，随后在近轴细胞、体节中表达；随着胚胎的发育，Myf5在成熟体节中表达量降低，在新生体节中表达较强；MyoD自30个体节时期后只在新生的尾部体节中表达，在成熟的体节中表达量降低；在孵化期，MyoD和Myf5在头部及鳍的肌肉、尾部的体节中表达；生长期的牙鲆中，Myf5在骨骼肌和肠中表达，成体牙鲆中，Myf5只在肌肉中表达；生长期的牙鲆及成体牙鲆中，MyoD只在肌肉组织中表达。

Next the otter shell portions of the sour bamboo shoots are removed the first inner portions are then cut into smaller pieces.

酸笋 sour bamboo shoots 买回来后，先将老的部分切除，再将酸笋拨成丝，把酸笋也切成小丁。

The humidity resistance and the sensitivity to chemical warfare agents simulants of the tuned films were also investigated.Self-assembled LBL polyaniline films were prepared from aniline monomers by in situ polymerization and the mechanism of film formation was discussed. It was proposed that the self-assembly of polyaniline occurred through the quick absorption of aniline monomer cations or aniline monomer cation radicals on the glass surfaces by electrostatic attractions to form homogeneous polymerization centers, then increased gradually by the way of upright to the glass supports for extensity, leading to the polyaniline chain growth. The polyaniline was doped under acidic condition to absorb anionic polyelectrolyte, such as sodium polystyrene sulfonate based on the electrostatic force, or to adsorb polymer based on hydrogen-bonding interactions, for example Polyvinyl Alcohol, and then recycled to fabricate_n or_n self-assembled multilayer films.

跟踪了原位聚合的具有纳米结构的聚苯胺多层自组装膜的成膜过程，提出了该自组装膜的成膜机理：聚合反应初始阶段的苯胺阳离子或苯胺阳离子自由基通过静电作用快速吸附到带负电荷的基片表面，形成均匀的聚合中心，然后以垂直于基片的方式进行链增长生成PANI；PANI在酸性条件下经现场掺杂后带正电荷，再通过静电作用吸附带负电荷的聚电解质如聚苯乙烯磺酸钠，或通过氢键作用吸附聚合物如聚乙烯醇，这样循环LBL-SA即得到_n或_n等原位聚合聚苯胺自组装膜。

The safety endpoint was the rate of sICH. Efficacy endpoints were the rate of reperfusion on MRI after 4 to 8 hours and clinical outcome as assessed by NIHSS, modified Rankin scale, and Barthel Index at 90 days.

弥漫成像能够显示梗死和已经死亡的组织，而灌注成像能够勾画出周围缺血的脑组织，但不包括死亡了的，这样就可以评价中风相关的血管再通后是否对患者有益。

This stone pagoda was built by using rocks as bricks. It was originally built as a 9-story pagoda but only 3 stories remain now. It is a part of very important data in the studies of the Silla Stone culture and development.

该塔系将石块打磨成砖块样后堆成，原来为九层，现仅存三层，为了解新罗石塔文化的发展史提供了十分珍贵的资料。

The evaluation model of special performance and special ability indexes are set up for Chinese elite men pole vaulter: y=-1.251+0.01921(60m race)－0.0104+0.001166+1.537(the height of athlete,s single arm-raising)+1.158+2.304×10-5－0.00278+3.83×10-4.

在专项能力结构中，8个指标的地位是：技术指标＞单臂举高＞垫上后滚翻展体成倒立＞60m跑＞垫上后空翻＞杠铃卧推＞跳远＞吊环摆上成支撑。

Adult rats were measured body weight and imaged by Micro-CT, then divide into four groups randomly; according to control, low dosage, medium doagse, high dosage. Each group was given aqueous solution of Paraquat via oral single dosage at 0mg/kg, 40mg/kg, 60mg /kg, 80mg/kg, respectively.In control group, the color and luster of the lung samples appeared normal and no petechia was observed.

成鼠先秤重及Micro-CT造影，再以随机方式分成控制、低剂量、中剂量、高剂量四组，依其组别给予单次不同剂量之巴拉刈水溶液，给药后每周进行两次秤重及Micro-CT造影，连续进行四周，并在每周造影后，每组随机牺牲一只成鼠，取出肺脏，进行检体及组织病理观察。

Raw cotton performance and key technology of each process of influencing on yarn forming neps were analyzed through spinning test.

成纱棉结是衡量纱线质量的一项重要指标，它不仅影响成纱和织物的外观，而且还影响后加工质量和生产效率。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。