后成

Detect that whether the ADSCs were differentiated inducedly into osteoblasts or adipogenic cells by Alkaline phosphatase staining、Von kossa staining and Oil red "O"staining after the ADSCs were cultured in inductive basic medium containing osteogenic induction agent and adipogenic inducers for four weeks.②.

①。采用密度梯度离心法加贴壁培养法对兔脂肪组织进行了分离纯化，CD44免疫组化法分析ADSCs表面标志；用含成骨、成脂诱导培养基培养4周后，行碱性磷酸酶染色、Von kossa染色及油红&O&染色检查ADSCs是否向成骨、成脂细胞定向分化。②。

The first synthetic route uses 1,2,4-trimethoxybenzene and chloroacetonitrile in forming 2,4,5-trimethoxy-a-chlor-acetophenone under the anhydrous condition, then the intermediate condensates with papaverine forming the core pyrro[2,l-a]isoquinoline, followed by formation and lactonization to form the lactone ring. The second synthetic route uses prepared aldehyde with prepared ethyl nitroacetate by Knoevenagel condensation to obtain 2-Nitro-3-(2,4,5-tris-methoxy-phenyl)-acrylic acid ethyl ester and 2-Nitro-3-(2,4,5-tris-benzyloxy -phenyl)-acrylic acid ethyl ester etal intermediates. The lamellarin skeleton could arise from condensation of the papaverine and these intermediates by Michael reaction, the ester group is provided for subsequent lactonization. The third synthetic route uses coumarin or indan-l,3-dione derivatives and papaverine to form lamellarin under basic conditions.

第一条路线首先从1，2，4-三甲氧基苯出发与卤乙腈作用合成卤代芳酮中间体，然后与罂粟碱反应合成开链片螺素，最后经乙酰化、去保护、成内酯环得到片螺素；第二条路线由制备的芳醛和制备的硝基乙酸乙酯经缩合得到2-硝基-3-芳基丙烯酸乙酯，然后由该中间体与罂粟碱反应，在完成关环的同时也引入酯基，最后去保护、成内酯环得到片螺素；第三条路线是由香豆素或茚二酮出发，经溴代后的中间体与罂粟碱反应，得到片螺素的基本框架。

To avoid the low yield in direct hydrosilylation addition of allyamine, 1, 1, 1, 3, 3, 3-hexamethyldisilazane is used to protect the amino groups, and the protective groups are removed easily after hydrosilylation addition, then the high yield of ladderlike copolymethyl-aminpropylsilsesquioxane was obtained.

为了克服将烯丙基胺直接与聚氢基甲基倍半硅氧烷进行硅氢加成反应接枝效率低的缺点，探索用六甲基二硅氮烷对烯丙基胺预保护，硅氢加成反应后再脱保护，从而以较高产率制备了含胺丙基、甲基的反应性梯形共聚倍半硅氧烷。

Results In define induction envioroment, HPMSCS can diferentiate into lipoblast、after 2 weeks, the induced cells are full of lipid droplet;HPMSCS can differentiate into osteoblasts, after 4 weeks, we can see calcified tubercle in the cells, calcium in the calcified tubercle can be dyed black by Von Kossa and ALP dying is positive reaction above 95%.

结果 HPMSCS在培养基中持续培养后，在特定诱导环境下，成功使其向成脂肪细胞转化，培养2周时，可见融合成团的脂滴充满整个细胞；成功使其向成骨细胞转化，培养4周以上可见明显钙化结节，von Kossa染色可将骨结节中沉积的钙染成黑色，碱性磷酸酶染色呈强阳性反应达到95%以上。

Results Infected by this peptide, cell viability decreased 28.9%. Under light microscope, cells were shrinked and rounded, many cells were divorced from plate wall, some neuraxon shortened and broke. Apoptotic cells which nucleolus shrinked and rounded could be coloured orange by fluorescent colouration. Under electron microscope, chromatin gathered along the inside of the nuclear membrane, vacuole bodies appeared. Apoptotic peak was detected by flow cytometry and the ladder band appeared in DNA electrophoresis.

结果：细胞接触肽段后存活率下降28.9％；光镜下可见细胞贴壁不良，胞体缩小，细胞突起断裂缩短；荧光染色可见细胞突起缩短、胞核固缩、胞质染成橘红色的凋亡细胞；电镜下可见胞质中出现空泡样结构，细胞染色质浓集于核膜内侧并裂解成碎块状；流式细胞仪检测细胞出现亚二倍体峰，DNA电泳出现梯状带。

Based upon the analysis and study of the signal-to-noise ratio of SAR imagery, this dissertation developed the coherent and noncoherent multilook imaging approach which suits various types of ground feature and target.

本文在回顾合成孔径雷达的发展历史、综述SAR的各种工作模式和条带SAR的各种数字成象方法后，研究了SAR实时成象中的一些问题，提出了解决的方法。

Cinnabarinus. 500 times dilution of the concentration on the poisoned Tetranychus cinnabarinus better, 96 h in drug corrected mortality of young mite reached 80%, the corrected mortality of the nymphae mite reached above 75%, the corrected mortality of the adult mites reached 60%, which showed that the SC had poisoned the role to all mites.

结果］稀释500倍以下的浓度对朱砂叶螨的毒杀效果较好，在药后96h幼螨的校正死亡率均达到80%以上，若螨的校正死亡率均达到75%以上，成螨的校正死亡率均达到60%以上，表明BtR05悬浮剂对朱砂叶螨的幼螨、若螨和成螨均具有毒杀作用。

Recent data suggested that Notch receptors and their membrane-bound ligands Delta and Serrate were involved in both patterning and cell fate determination during odontogenesis.

越来越多的证据表明：Notch途径参与了牙齿发育的各个过程，包括牙齿早期发育细胞的增殖、分化过程，决定细胞的定向分化方向，决定成牙本质细胞与成釉细胞的终末分化命运，并且参与牙齿炎症损伤后修复过程，与第三期牙本质的形成有关。

In order to check the corresponding compound at 31P NMR –45.4 ppm, stable pentacoordinate spirobicyclic 2-phenoxy-1,3-phenylene-dioxo-1,3,2-iminoacetoxyphosphoranes were synthesized through a new and efficient method whereby phosphorus pentachloride was displaced stepwise by catechol, N,O-bisamino acid and phenol, or catechol, phenol and N,O-bisamino acid, their 31P NMR chemical shifts are at about 31P NMR –45.0 ppm, the results showed that the compound 4 at 31P NMR –45.4 ppm was pentacoordinate phosphorane containing amino acid residue.

采用磷谱跟踪来研究上述成肽反应机理。例如在氮气保护下，0.1mmol O，O-亚苯基磷酰氯（1）和0.15mmol N，O-二苯丙氨酸（2）苯溶液加入0.6ml核磁管中，混合均匀，反应过程用磷谱检测。反应1分钟后，起始原料1(δp=19ppm)很快转变成N-磷酰苯丙氨酸的三甲基硅基酯（3）(δp=19.8ppm)，在8分钟内，3完全转变成苯丙氨酸磷烷4(δp =-45.4ppm)，同时出现环磷三酯（6）(δp=3.5ppm)。随着反应的进行，对应于化合物4的峰逐渐变小，而6逐渐变大。

Results The hEFs were successfully isolated and cultivated from gonadal ridges and dorsal mesenteries of human embryos. They could be passaged beyond the 25th generation. The biologic characteristics of the cells did not change, even in high-passage cells or frozen-thawed cells. The cells expressed prolyl 4-hydroxylase β, but not cytokeratin-4, which was similar to the fibroblasts. The cultured cells expressed bFGF and LIF.

结果：从人胚胎生殖腺嵴和肠背系膜中成功地分离培养出hEFs，该细胞可传25代以上，且经过传代及冻存复苏后生物学特性无改变；hEFs表达脯氨酰4-羟化酶β亚单位，不表达细胞角蛋白4，确定其为成纤维细胞；该成纤维细胞表达bFGF和LIF。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。