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The result of drip test (The height is 20 cm) was that the bibulous speed was 4 to 5 seconds.

先用4公升实验室的管道自来水,与40克洛醇配成水溶液,剪一块30 x 50 cm 的布料,以手工操作,30分钟后,再换4公升新配的水溶液,30分钟后,再换4公升新配的水溶液,30分钟后烘乾,经检查,棉子売清除达98%,滴水测试结果是(高度20 cm )吸水速度4 -5秒,白度接近6。

Methods Animals were randomly and equally divided into five groups: saline control group, positive drug control group, low-dose, medium-dose and high-dose crocetin group. Animals were anesthetized with urethane (1.2 g/kg), then followed by the perfusion of aconitine (10 μg/mL) through caudal vein in rats, or of ouabain (9 μg/mL) through external jugular vain in guinea pigs in a constant velocity of 0.1 mL/min, and the emergence time of ventricular fibrillation, ventricular extrasystole, ventricular tachycanlia and cardiac arrest were then detected and recorded. The recorded time was then translated to the cumulated amount of aconitine and ouabain. Experimental arrhythmia was induced by rapid injection of 4% CaCl2 (140 mg/kg), and the emergence time, persistence time, incidence and death of arrhythmia after the administration were observed.

将实验动物随机分为生理盐水组,阳性药物对照组,藏红花酸高、中、低剂量组,SD大鼠采用1.2 g/kg乌拉坦麻醉后,从尾静脉匀速(0.1 ml/min)灌注10μg/ml乌头碱溶液,豚鼠麻醉后从颈静脉匀速(0.1 ml/min)灌注9μg/ml哇巴因溶液,观察出现室性期前收缩、室性心动过速、室颤和心脏停搏的时间,然后换算成乌头碱和哇巴因的累积量;快速推注4%氯化钙(140 mg/kg)诱导大鼠心律失常,观察给药后心律失常出现时间、持续时间、心律失常发生数及死亡数。

The notable proliferation was not observed by eyes in the local of injection. The infiltration of inflammation cells and mild proliferation of fibrocyte around dura mater was observed by HE stained in 4 and 8 weeks after injection. Infiltration and exudation of inflammation cells was observed by HE stained in epidural nerve root. Compared with group A, no changes of group B, C and D were observed under specific stained. Proliferation of type Ⅱ collagen fibers around dura mater was seen under immunohistochemical stained in 4 and 8 weeks after injection. There is no significant demyelination changes under LFB stained. The thickness and shape of the myelin sheath in epidural nerve root was not regular under transmission electronic microscopy in 4 and 8 weeks after injection. Fibroblast was also seen there. In nerve endometrium, macrophage could be seen under TEM, myelinated nerve fiber changed significantly, but nonmyelinated nerve fiber changed mildly. When 8 weeks, the changes of group D is smaller than the group B and C.

给药局部肉眼观察未见明显的纤维组织增生;HE染色可见B、C、D三组给药后四周及八周时硬膜内外均有炎细胞浸润,纤维细胞轻度增生,硬膜外神经根内有炎细胞浸润及炎性渗出;特殊染色B、C、D三组同A组相比未见有脊髓及神经根的改变;免疫组化染色,给药后四周及八周时,硬膜内外均有Ⅱ型胶原纤维增生;固兰染色B、C、D三组未见有明显脱髓鞘改变,与A组相比无明显异常改变;电镜观察B、C、D三组在给药后的四周及八周时,表现为硬膜外神经根内髓鞘厚薄不一,形状不规则,可见成纤维细胞,神经内膜中可见有巨噬细胞;粗大的有髓神经纤维变化明显,无髓神经纤维受累较轻;八周时电镜下D组改变较B、C两组为轻。

Results Compared to those before treatment the volume of the implanted keloid of Group D began to decrease since 14 days after treatment time-dependently (all P 《 0.05), and the volumes of the other 3 groups continued to increase and peaked on days 21, 14, or 7 respectively (all P 《 0.05).Microscopy showed infiltration of a larger quantity of histiocyte in the keloid tissue, and more obvious collagen disorganization and apoptosis of fibroblasts in Group D than in the other 3 groups.The protein expression of Bcl-2 was more remarkable and the protein expression of BAX was less remarkable in Group D than iu the other 3 groups.

结果 用药前和用药后7、14、21、28、35 d,D组瘢痕疙瘩组织块体积(mm3)分别为173±5、172±5、147±5、125±6、112±7和84±9,从用药后14 d开始明显缩小(均P<0.05);而其他3组瘢痕疙瘩组织块体积均明显增大,从用约后7 d开始各时点测得的体积均明显大于D组(均P<0.05)。D组瘢痕疙瘩组织中有大量小鼠组织细胞浸润,胶原结构破坏和成纤维细胞凋亡明显重于其他3组,Bcl-2蛋门质表达明显弱于而Bax蛋白质表达明显强于其他3组。

Results一. Culturing and identification of human conjunctiva cells1. morphous of conjunctiva cellsCells were harvested by tissue cultivation after digestion. Ponderosus cells adhered after 48 hours. Cells were shown in shape of round, ellipse and polygon. Cell body was loose and lucency with nucleus in center, cell membrane was also clearly seen. Cells were arranged in inlay and fuse in film after 12-14 days.2. Immunochemistry testNomogeneous immunochernical positive granules against CK13 were observed in endochylema of human conjunctiva cells.3. growth curve of conjunctival cellsSubcultured cells were aged and feeble after 5th passage.

结果一、正常人结膜细胞的培养与鉴定1、活体细胞的形态消化后组织块培养法:48小时后见大量上皮细胞贴壁,细胞不规则呈圆形、椭圆形、多边形,细胞体肥大透亮,细胞核位于中央,胞膜清楚,约12-14天后细胞呈镶嵌状排列,融合成膜状。2、免疫组化鉴定培养的人结膜上皮细胞CK13免疫组化染色见阳性颗粒均匀分布于胞浆中。3、培养结膜细胞生长曲线传代培养多数样本于P5代以后开始出现衰老征象。

Bonding card board (English name: PLXwood) logs are planed into a 1.6mm-2.0mm between the single board, high temperature drying moisture, up to a certain standard, into the system board workshop preparation chemical raw materials, manufactured according to customer requirements nine, fifteen, eighteen different specifications, such as multi-layer plywood, and then after a high temperature, high pressure, hot pressing machine to suppress made with molded plywood processed into the Pallet is laminated card board.

胶合卡板(英文名字: PLXwood )是将原木刨成 1.6mm-2.0mm 之间的单板,经高温烘干水份,达到一定标准后,进入制板车间配制化工原料,根据客户要求制成九、十五、十八等不同规格的多层胶合板,再经过高温、高压、热压机压制而成,用成型的胶合板加工成的栈板就是胶合卡板。

On both sides of the head, bo palletizer gables and jump out after all, coping with brick and WaiHu bolts into the curve, delicate and elegant, In front of the hall YanXia is WangZhuan grinding and Chuan bent with very precise porch corridor, look up, its monumental made by artificial and materials that, And the bridge of short column and column, is not a simple shu to use a few short wood, but installation high-relief curving flowers.the curving flowers and leaves, Lu carved into a cloud, LiangYun fog, not only can satisfy the structural component of the requirements, and the proper decoration, try and do not provide craftsmanship.

两侧山墙的垛头、博缝及后檐出跳,也都用砖磨成道道内、外弧的曲线,精致而又素雅;厅屋前檐下是把望砖磨成与弯椽配合得极为精确的轩廊,抬头望去令人赞叹不已,其制作所耗费的人工和材料可想而知;还有那梁架上的短柱与蜀柱部位,不是简单地用几根短木,而是安装高浮雕和透雕雕刻成荷叶墩、栌斗、山雾云、抱梁云构件,不仅能满足结构上的要求,又恰到好处的进行了装饰,真可谓两全齐美,别具匠心。

So from the aspects of economical and social benefit, it has a lot of advantages. On the other hand CFG pile with long screwy drill pipe inner pump pressure is low noisy and without muddy water pollution; The process of drilling will not lead to vibration and has the capacity to penetrate hard rock layer; Also the construction is of high efficiency.

并且长螺旋钻管内泵压成桩工艺具有低噪音,无泥浆污染;成孔制桩时不产生振动;成孔穿透能力强,可穿透硬土层,诸如沙层、圆砾层和粒径不大于60mm的卵石层;施工效率高等优点,所以该技术在全国推广后,得到了广泛的应用,目前已成为应用较为普遍的地基处理技术。

Controlled by the computer, the device can transport a sample to be subjected to parameter extraction to the electrical rotation stage through the pipeline system, the CCD imaging system takes the plant image at a single angle, then the images are subjected to image segmentation by the computer, the projection plant height at the angle can be obtained after subjecting the divided binary images to skeleton extraction and leaf path searching, and finally the maximal value of the projection plant heights at all angles is calculated as the plant height.

本发明涉及植物株高自动测量装置及测量方法,本装置包括流水线系统、可见光CCD成像系统、电动旋转台、旋转台控制器、计算机、图像采集卡,电动旋转台依次与旋转台控制器和计算机相连,可见光CCD成像系统依次与图像采集卡和计算机相连,本发明由计算机控制,采用流水线系统,将待提取参数的样品送上电动旋转台,由CCD成像系统拍摄单个角度下的植株图像,由计算机将所得图像进行图像分割,从分割后二值图像经过骨架提取和叶片路径搜索可得到该角度下投影株高,最后求出所有角度下投影株高的最大值作为植株的高度。

The quantity of CD3+ cells in the EdNSC, AdNSC of cloning goattransplantation group and control group had no statistics difference(P>0.05) at acute and chronic period.Overall, in our study, We got the conclusions as follow: 1,The EdNSC, AdNSCof cloning goat and the AdNSC of the non-cloning goat were successful obtained;EdNSC growed faster than AdNSC in the same cell culture method. 2,The braincontusion and laceration model of cloning goats were successful established by freefalling of 50 gram of steel ball accelerate strike on the goat brain from 100cm height.3, EdNSC and AdNSC transplantation both had obvious therapeutic effect on braincontusion and laceration of cloning goats; The therapeutic effect of EdNSCtransplantation was better. 4, Allograft of stem cells has acute and chronicimmunological rejection and autografting of stem cells has no acute and chronicimmunological rejection.

在相同的条件下,EdNSC体外培养速度比成AdNSC快。2对羊脑上薛氏沟周围运动皮层区使用50g×100 cm力度作自由落体加速撞击,可产生观察指标明确且存活率高的羊脑挫裂伤模型,有望广泛用于神经干细胞移植及药物等治疗的实验研究。3胚胎神经干细胞及成体神经干细胞移植治疗脑挫裂伤后均有较明显的治疗效果;二者相比较而言胚胎神经干细胞稍好于成体神经干细胞。4中枢神经系统异体神经干细胞移植存在着明显的免疫排斥反应,而自体神经干细胞移植基本无免疫排斥反应

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