后成

Results①The formation of posterior capsule opacification in rabbit eyes: observation in different stages after the operation showed the inflammatory response remarkable at the first three days with iris congestion; edge of anterior capsule circularity opacification at the 1st week, but no remarkable change in the posterior capsule; just gauze-like at the 2nd week; strip-like opacification at the end of the 1st month, funicular opacification after 2～3 months, while visible opacification at the 5th month after the operation.

结果：①兔眼后发性白内障形成情况：术后不同时期裂隙灯下观察可见术后前3d前房内炎症反应明显，虹膜充血；术后1周可见前囊截缘出现白色环状混浊，但后囊尚透明；术后2周后囊呈薄纱状；1个月出现线条状混浊，2～3个月成条索状混浊，术后5个月后囊明显混浊。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

And then adding NAA at low concentration in MS was used to give rise to roots. We also found that lower level of hormone could control effectively browninng and vitrifaction during the culture and G1 n (6mg/L) and AgNOj (2mg/L) supplemented in shoot induction media could improve the shoot information rates apparently (about90%).The whole period of plant regeneration from leaflets of peanut could be divided as five steps: germination - shoot induction -shoot elongation-rooting- tranplant.

用1／2MS培养基萌发花生种子，9-10d后，从无菌花生苗上切取幼嫩叶片中部为外植体。2500Lux光照和27±1℃条件下，在诱芽培养基(MS+BA3mg／L+NAA0.8mg／L+AgNO_32mg／L十Gln 6mg／L)培养12-14d即可观察到明显芽点或瘤状突起，较前人报道的培养时间大大缩短了，4w后芽点进一步发育成丛生芽，芽诱导率达90.2％，每个外植体平均产9个丛尘芽，然后转至培养基MS+BA3 mg／L+AgNO_32 mg／L上诱导芽的伸长，3-4w后可长至3-4cm，切下带有2-3片叶片的幼芽移至生根培养基(MS+NAA0.8mg／L+AgNO_32mg／L)，1w后切口处可见白色不定根形成。

Bronchiolitis obliterans is the most disturbing complication following lung transplantation. This study is to investigate whether prolonged ischemia would promote early establishment of BO Material and Methods Left lower lobectomy was performed from miniature swine. Lung fragments, each measured 1 cm3, contained terminal bronchioles were auto- or alloimplanted into the subcutaneous area around the nipples. These lung implants were removed on 7th; 14 th; 21 th; 28th and 56th days and were fixed for histological examination. Experimental group using the same procedures but the left lower lobe was washed with 4.degree.C Lactate ringer via interlobar artery before lobar bronchus was resected.

肺移植后的阻塞性细支气管炎是肺移植后最困扰的并发症，本研究的目的希望了解缺血时间的延长是否会促进阻塞性细支气管炎提早发生实验方法施行小猪的左下肺叶切除手术，将取下的肺切成含终末支气管1 公分立方的肺小块，以自体及异体异位肺移植模式，将植入小猪皮下的肺小块於植入后第7、14、21、28 及56 天取下，在病理下检示其病理变化，并利用原位灌留的方式将左下肺叶以4℃林格氏液灌洗后再摘除之，观察有无灌洗对於阻塞性细支气管炎生成的影响。

Results:Out of the nine tuberculose patients,six healed after second operation,one sinus formation,two died.Out of the two disruption of wound,one was re-sutured and healed,the other healed after a long term of dressing changing.Seven infectious incision were controlled within one month.Out of the nine cerebrospinal fluid fistulae,eight were cured conservatively,only one spinal meninges was repaired.One epidural hematoma was cleared and made a good healing.Out of the two mold infection,one was cured after focal clearing,the other developed to chronic recurrent sinusitis.Four patients with sore healed after decompression.The other eight patients (four by hemostasis narcotic,two by liquefied fat,two by dermal allergic papular eczema) had good healing after intra-red therapy.

结果：9例结核患者再次手术后6例愈合，1例窦道形成，2例死亡；切口裂开2例中1例经切口再缝合愈合良好，另1例经10个月长期换药切口愈合；7例切口感染者均在1个月内控制感染；9例脑脊液漏，其中8例经保守治疗愈合，1例行脑脊膜再次修补术；1例硬膜外血肿清除后愈合良好；霉菌感染2例，其中1例经病灶清除后切口愈合，另1例演变成复发性窦道炎；4例压疮去除压迫后溃疡愈合；另外8例切口延迟愈合者中止血水引起者4例，脂肪液化2例，皮肤过敏2例，均经红外线照射后切口愈合。

When I had done this, I began to work my Way into the Rock, and bringing all the Earth and Stones that I dug down out thro' my Tent, I laid 'em up within my Fence in the Nature of a Terras, that so it rais'd the Ground within about a Foot and a Half; and thus I made me a Cave just behind my Tent, which serv'd me like a Cellar to my House.

做完这些工作后，我又开始在岩壁上打洞，把挖出来的土石方从帐篷里运到外面，沿篱笆堆成一个平台，约一英尺高。这样，帐篷算是我的住房，房后的山洞就成了我的地窖。

The second approach ingredients: flour ingredients: scallions tender meat a little rapejiang suan heart a little practice: 1, warm water and soft PowerPhone and more and after a good 5-7 minutes Add basin line 2, scallions fine cut of meat Stand-slice 3, the line mian zhang sharing with good flour into a pie, a knife slip into a three-finger width of article 4, under the boiling water into the surface film will stretchjiang suan in 6,7 minutesru guo be mature plus nine patches salt, Add Rape Heart 7, points to the vinegarqi guo 8, surface-chip Add shallot sauce

第二种做法主料：面粉配料：香葱嫩油菜心姜蒜少许肉片少许做法：1、温水和面面和的软些和好后放入面盆行5－7分钟 2、香葱切细肉切片备用 3、将行好的面粉用擀面杖摊成饼状，用刀滑成三指宽的条 4、滚水下入姜蒜将面片抻入锅中 6、7分钟后待面片九成熟加盐，放入油菜心 7、点入香醋起锅 8、面片上放入香葱调味

Production: first eggs cooked, let cool after the peeled and cut with a knife to the egg, and then remove the egg yolk; and then cut into the woods at the end of half an egg-shaped, to stand on the plate; the salad dressing and after the egg yolk mix, then put into the egg-ri, the last raisins and fruit will be placed on the fait accompli.

制作：先将鸡蛋煮熟，放凉后去壳，并用刀子把蛋切开，然后把蛋黄取出；再把半个蛋底切成林子状，让其站在盘子上；把沙拉酱和蛋黄拌匀后，装入蛋林里，最后将葡萄干和果料放上即成。

Inseminated in 8-9 hrs after GVBD, the ova have the highest hatching rate (more than 86% in the concerned spawn).Based on the above insemination research of the oocyte maturation in vitro, recombinant opAFP-pUC19 was microinjected into the germinal vesicle of oocyte of maturation in vitro.

经过卵母细胞时期在生发泡中注射外源抗冻蛋白基因的体外成熟卵，其受精率（70～80%）和孵化率（38～75%）均低于对照组10～30%，在进行外源抗冻蛋白基因注射后授精孵出的小鱼长至半成体和成体鱼后，分别用斑点杂交和Southern blo-tting分析外源基因的整合。

In order to confirm this suspect, we choosed a cultured rat granulation tissue fibroblasts model in vitro and applied RT-PCR and cell-ELISA technology to detect the changes of firbroblasts intrinsic EGF, bFGF, TGFβ-1 with their receptors gene expressions and protein synthesises after stimulated by SP. Our purpose was to explore the possible effects and patterns imposed by SP on fibroblasts intrinsic growth factors and their receptors expressions, which maybe offer theoretical basis for promoting wound healing via improving nervous functions and regulating neuropeptides secretions.

为进一步验证这一推测，同时排除在体多因素干扰，我们采用了一种大鼠肉芽组织成纤维细胞体外培养模型，采用RT-PCR与细胞ELISA技术，检测SP刺激成纤维细胞后，成纤维细胞内源性EGF、bFGF、TGFβ-1及其受体基因表达和蛋白合成的改变情况，探讨SP对成纤维细胞内源性生长因子及其受体表达的影响以及方式，以期为经由改善神经功能、调节神经肽分泌途径促进伤口愈合提供理论依据。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。