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RESULTS: BMSCs changed from spindle into ellipse and reniform after osteogenic induction.

结果:骨髓间充质干细胞经成骨诱导后,由长梭形变为椭圆形及肾形。

RESULTS: The passaged MSCs exhibited uniform appearance in fusiform shape, and their growth was slowed down after 9 passages, exhibiting cytoplasm vacuolization and body enlarging.

结果:传代培养的骨髓间充质干细胞形态均一,呈梭形、纺锤形,克隆样生长,培养至9代后生长减慢,并出现胞浆空泡化、胞体变大等老化现象。

As exposed to optics microscope and inverted microscope,compared to control,SW480 cells with Giemsa staining that treated with the H2RA(cimetidine、nizatidine) took on malignance declining as crimpled firstly,cellular bulk and heteromorphism dimimished,the amount of rounded cell manifolded,nucleolus bulk lessened,dyeing thin,the amount of nucleolus decreased,partial cell organs manifest side-gathering phenomenon on chromatin,and the link between SW480 cell is loosened , subsequently partial rounded cell is floated.When the H2RA(cimetidine 40μM、nizatidine 20μM) against SW480 cells after 3 day,SW480 cells change roundly,nucleolus smashed,shrinking nucleolus came into being,and part of those not adhibited cell wall.

在光镜和倒置相差显微镜下,与对照组相比,经姬姆萨染色观察,西咪替丁、尼扎替丁处理的SW480细胞,先发生皱缩,体积变小,异形性减少,细胞变圆数目增多,细胞核变小,染色变淡,核仁数量减少,部分可见染色质边集现象;细胞间连接疏松,随后部分变圆细胞浮起。40μM西咪替丁及20μM尼扎替丁连续处理3天后,SW480细胞全部变圆,细胞核碎裂,固缩微核形成,部分不再贴壁。

RESULTS AND CONCLUSION: After passaged, BMSCs were grown quickly with long-fusiform shape.

结果与结论:传代后骨髓间充质干细胞生长迅速,呈长梭形,形态良好。

Screening should generate an even after Rose leaf-shaped structure, and the world could not make a moire patterns these mesh point structure will pass a small image details and see the wide range of halftoning, produce high quality images.

增网后答该生不败平均的玫瑰斑形的网不面布局,而不克不及生不败莫尔纹,这些网不面布局不兵传达洪不小的图像梗概和广宽的阶调边界,制息不入矮质度的图像。

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