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We studied an additional 7 rabbits after left entricular endocardial cryoablation, resulting in a thin layer of suriing epicardium. Cai sinkholes occurred 31±12 ms after the shock, followed in 19±7 ms by first postshock actiation in 63 episodes of unsuccessful defibrillation.

我们进一步研究了左室内膜冷冻消融后的7只兔,Cai穴发生在电击后31±12ms,在63次除颤未成功情况下,首次电击后激活出现在电击后19±7ms的Cai穴。

Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, which indicates PX and melatonin supplementation have no significant effect on CRH levels.

结果: ①松果体摘除后大鼠下丘脑室旁核CRH 原位杂交和免疫组化的结果与假手术对照组相比均显示阳性细胞数增多、阳性面积增加、平均灰度降低,提示松果体摘除后下丘脑CRH 神经元的表达增强;②松果体摘除大鼠补充褪黑素后,下丘脑CRH 原位杂交和免疫组化染色增强呈剂量依赖性恢复,不过不论是低剂量组还是高剂量组,也不论是术后4 周还是术后8周,均不能恢复至正常;③松果体摘除后神经垂体正中隆起CRH 免疫组化结果显示阳性面积增加、平均灰度降低,提示下丘脑CRH 神经元释放CRH 增加;④ELISA 法检测血清CRH结果示各组血清CRH 浓度差异没有显著性,表明松果体摘除及补充褪黑素对血清CRH 水平没有显著影响。

Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, whichindicates PX and melatonin supplementation have no significant effect on CRH levels.

结果: ①松果体摘除后大鼠下丘脑室旁核CRH 原位杂交和免疫组化的结果与假手术对照组相比均显示阳性细胞数增多、阳性面积增加、平均灰度降低,提示松果体摘除后下丘脑CRH 神经元的表达增强;②松果体摘除大鼠补充褪黑素后,下丘脑CRH 原位杂交和免疫组化染色增强呈剂量依赖性恢复,不过不论是低剂量组还是高剂量组,也不论是术后4 周还是术后8周,均不能恢复至正常;③松果体摘除后神经垂体正中隆起CRH 免疫组化结果显示阳性面积增加、平均灰度降低,提示下丘脑CRH 神经元释放CRH 增加;④ELISA 法检测血清CRH结果示各组血清CRH 浓度差异没有显著性,表明松果体摘除及补充褪黑素对血清CRH 水平没有显著影响。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry,FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时,对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后,Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR,RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时,Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope,LSM)观察北豆根总碱(0、40μg/ml)作用24小时后,Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

The results showed that orexin-like immunoreactiveneurons were observed in the medial preoptic nucleus,periventricular nucleus,paraventricular nucleus,supraoptic nucleus,dorsomedial nucleus,perifornical nucleus,mammillary nucleus,anterior area,lateral area and posterior area of the hypothalamus,with majority in the lateral area,supraoptic nucleus and mammillary nucleus and with minority in t...

结果表明,在猪下丘脑内,开胃素A免疫阳性神经元分布于下丘脑的视前内侧区、室周核、室旁核、视上核、背内侧核、穹隆周核、乳头体核、前区、外侧区和后区等部位,以下丘脑外侧区、乳头体核和视上核出现的免疫阳性神经元最多,以室周核的最少。这一结果与在其他动物上获得的基本相似。

The incision of right ventricle was closed directly in 90 cases, in 52 cases only right ventricle outflow tractwas widen, RVOT and main pulmonary were enlarged separately with two patch in 2 cases ,and transannular patch was implanted in 171 patients.

318例TOF根治手术中,90例修补室缺后直接缝合右室切口,52例补片加宽右室流出道;2例用两块心包补片分别加宽主肺动脉和瓣下流出道;行跨肺动脉瓣环补片171例。

The fire coal falls on the chain fire grates from coal: bucket and hen goes into the furnace tank for burning, and the flame passes through the upper back arch of the combustion chamber and back snloke chamber and then through the second fire pipe Io the

燃料煤自煤斗落在链条炉排面上,进入炉膛燃烧后,火焰经过后拱上部燃烧室和后棚管烟室后进入二回程烟管到前烟箱,再由前烟箱折回第三回程管束后,通过热管空气预热器、多管除尘器,然后由引风机抽引经过烟囱排出。

The incidence of intra-atrial aberrant conduction post-premature atrial systole, premature junctional systole, premature ventricular systole, pararrhythmia, short duration of supraventricular tachycardia and atrial fibrillation were 63.7%, 14.3%, 11.0%, 7.7%, 2.2%, 1.1%, respectively. Of all the cases, 74.9% suffered from cardiovascular disease, 62.8% were patients older than 60 years with cardiovascular disease, 12.1% were complicated by heart failure.

结果 总发生率为0.43%,房性早搏后引起者占63.7%,交接性早搏后占14.3%,室性早搏后占11.0%,并行心律后占7.7%,短阵性室上速后占2.2%,短阵性心房颤动后占1.1%。74.9%有心血管疾病,60岁以上老年心血管病患者占62.8%,12.1%合并有心衰。

Air shower transmission Windows is a gate lock and the dual role, is passed Items must be after the cleanroom can enter after, so can effectively To prevent transmission of the item will dust or bacteria into the clean room.

风淋室传递窗是具有闸门与门锁的双重作用,被传递的物品必须经过风淋后才能进入洁净室,所以能有效地防止传递的物品将灰尘或细菌带入洁净室。

Including fuel and possesions insurance Standard Contract Length Standard Self-catered Room Large Self-catered Room internet access Single person flat Single Room Self-catered: Approx Standard Plus Self-catered Room Payment by instalments is only possible if students have a guarantor resident in England or Wales,: otherwise payment in full for the academic year is required at the start of the tenancy Standard en-suite room Single Studio Apartment Quay Point - Premium single or double occupancy Premium room self-catered Standard en-suite room individual TV licence

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。