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METHODS: Two polypeptides named peptide 1 and 2 were synthesized based on the bioinformatics analysis of the sequence of hMR-1 by using software TMHMM and DNAStar, then coupled with keyhole limpet hemocyanin for immunization.

TMHMM、DNAstar等软件分析hMR-1蛋白序列,选取2段优势抗原序列合成多肽并与钥孔戚血蓝素偶联,混合后免疫家兔。

The yield and purity of each DNA sample was tested by UV-spectrophotometer, the integrality of these DNA samples were run electrophoresis on the agarose gel. Each DNA sample was subjected to PCR amplification and hybridization using One lambada rSSO HLA-A,-B and -DRB1 commercial kit, the fluorescent intensity for positive bead and negative bead hybridized with HLA-A,-B and -DRB1 PCR products were calculated and analyzed.

使用2ml深孔板和TECAN DNA全自动工作站,从288份骨髓捐献者样本中提取基因组DNA;提取的DNA样本用紫外分光光度仪测定其浓度和纯度,并采用琼脂糖凝胶电泳检测DNA的完整性;DNA样本用One lambda rSSO HLA-A、B和DRB1基因分型试剂盒进行PCR扩增、分子杂交和Luminex流式磁珠分析仪检测,统计分析每一DNA样本HLA-A、B和DRB1基因扩增产物经DNA探针分子杂交后的阳性磁珠和阴性磁珠的荧光信号强度。

In order to effectively control fabric quality in the wet processing such as dyeing, printing, finishing and design a fabric which has better thermal-moisture com fort, this paper has proposed the theoretical basis and method for measuring capillary radius distribution along fabric plane based on the equilibrium between Laplace pressure of concave in front of capillary liquid and hydraulic pressure of liquid column by weight classification differentials.

为有效控制染色、印花、后整理等织物湿加工过程中的产品质量并设计出具有较好热湿舒适性能的服装面料,根据在织物毛细孔中液体前沿弯月面的Laplace压力和液柱静压间的平衡关系,提出基于质量分级和差减数据测定沿织物平面毛细孔径分布的理论依据和方法。

Cells from a human lens epithelial cell line(SRA01/04) were cultured in vitro till confluenced.

材料和方法:体外培养人晶状体上皮细胞株(SRA01/04)于含10%小牛血清的DMEM培养基中至细胞长满融合,胰酶消化后均匀种在六孔板中。

The long V block putted in the arch ribbed plate to limit two degrees of freedom in x axis and the z axes two degrees of freedom, the iron putted in the hole end limit to the degree of freedom which circle the y rotation of axis, the supporting block putted under the ribbed plate to limit degree of freedom along the y axis migration.

长V型块置于拱形肋板后限制x轴的两自由度和z轴的两自由度,挡铁置于孔端限制绕y轴旋转的自由度,支承块置于肋板下方限制沿y轴移动的自由度。

Four Kirschner pins were thrusted in cortex from lateral and dorsal,the pin tails were fixated in pores of external fixator.

手法复位后,将固定器固定于皮肤外侧,以4枚克氏针从侧方斜穿至骨皮质内,针尾固定在固定器上螺孔内。

Antistatic garment is applies Belltron9R series compound filature technology,which fully mixes conductive carbon power and melted nvlon at first;and then compound the base nylon with the mixture through specialfilament spraying hole,thus forming one conductive fiber with two components--the surface coat is layer of superthin ultra conductivepolymer while the inner core pure Nylon-6 polymer.

防静电工作服采用Belltron9R系列复合纺丝技术,将导电的碳粉与熔融状的尼龙充分混合后,经过特殊的喷丝孔与基体尼龙复合成纤,形成了双组分的导电纤维。其外层为具有极高导电性能的超薄皮状导电聚合物,内部则是尼龙-6聚合物。

METHODS: Sixty patients undergoing elective unilateral TKAunder spinal anesthesia received in a randomized, double-blindmanner a femoral, obturator, or sham nerve block at the endof surgery.

60名在脊麻下择期行单侧膝关节成形术的患者,在手术结束后接受随机双盲的股神经阻滞或闭孔神经阻滞或未作神经阻滞的临床研究。

Methods All 380 patients with cerebral palsy, admitted from January 2003 to March 2008, were studied retrospectively. Based on the position of spasticity, selective peripheral denervation was performed, including spinal dorsal root of lumbosacral region, sciatic nerve, obturator nerve, peroneal nerve.

回顾2003年1月至2008年3月收治的380例脑瘫患者,根据痉挛部位不同,选择相应周围神经进行部分切断,包括:腰骶段脊神经后根、坐骨神经、闭孔神经和胫神经。

Diesel engine oil pump gear shaft and bushing wear and tear take the initiative to repair oil pump gear as a result of its own weight and when the passive gear meshing radial thrust, shaft and bushing easily have side-hole grinding, increased gap.

柴油机齿轮式机油泵主动轴与衬套磨损后的修复由于机油泵主动齿轮本身重量及被动齿轮啮合时产生的径向推力,容易使轴和衬套孔产生偏磨,间隙增大。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。