后孔

METHODS: Normal human BMSCs were isolated and cultured by the whole bone marrow method. Cells of the third passage at 1×108/L were incubated on coverslip in a 6-well plate, and randomly divided into 3 groups: osteogenic induction group, which was added with primary medium and osteogenic inducer, supplemented with 10-8 mol/L dexamethasone, 10 mmol/L β-phosphoglycerol and 50 mg/L vitamin C; leptin osteogenic induction group was added with 50 nmol/l leptin in addition to osteogenic inducer. Cells in the blank control group were only treated with LG-DMEM containing 10% fetal bovine serum.

采用全骨髓法体外分离培养人骨髓间充质干细胞，传至第3代以1×108 L-1密度接种至预置盖玻片的6孔培养板中，设立3组：成骨诱导组添加原代培养液后，再加入含10-8 mol/L地塞米松、10 mmol/L β-磷酸甘油、50 mg/L维生素C的成骨诱导培养基；成骨+瘦素诱导组添加成骨诱导培养基后，再加入50 nmol/L瘦素；空白对照组仅加入含体积分数为10%胎牛血清的LG-DMEM培养液。

The recharge has designed with protect,while insent the output jack to electrical source,show green light,then insert the input jack to the lamp's recharge holl, show red light,it means work,Hours latter gree light again,it's full of the battery.

充电器内置充电保护装置，当充电器输入端接通电源指示灯为绿色，插入电筒充电孔后，指示灯为红色表示正在充电，电池充满电后指示灯又转换为绿色。

Methods The retinae of 16 postnatal 2～3 day Sprague-Dawley rats were dissected into cell suspension with trypsin digestion.

采用胰酶消化法将16只生后2～3天的Sprague-Dawley 大鼠视网膜制成细胞悬液后，接种于涂以鼠尾胶原的24孔培养板，预先置入1 cm×1 cm的载玻片。

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

In the maximal axial pulling-out force group, the hollow nails were directly implanted into 10 femoral neck samples after drilling at the axial center.

最大轴向拔出力实验分组，10个股骨颈在轴向正中钻孔后直接置入空心钉，另10个股骨颈在轴向正中钻孔后注入聚甲基丙烯酸甲酯骨水泥，然后置入带侧孔的空心钉。

In the maximal axial pulling-out force group, the hollow nails were directly implanted into 10 femoral neck samples after drilling at the axial center. The polymethyl methacrylate bone cement was inserted into other 10 femoral neck samples after drilling at the axial center, and the side-hole hollow nail were then implanted.

最大轴向拔出力实验分组，10个股骨颈在轴向正中钻孔后直接置入空心钉，另10个股骨颈在轴向正中钻孔后注入聚甲基丙烯酸甲酯骨水泥，然后置入带侧孔的空心钉。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

Based on the experiment, the flow variation and impact on release works is studied. The vortex theory is adopted to study the side shrinkage angle and optimum step of the bulkhead gate to improve the overflowing condition.

通过试验分析研究了闸后水舌的流态变化规律及其对泄水建筑物的影响；采用涡流理论分析研究闸后水舌侧收缩角的计算方法；根据侧收缩角计算出闸门槽的最优错距，从而改善闸孔出流的水流条件。

The backer card will be judged against creasing, bending, rolling, tearing, scuffing, scratching, lifting, print marks, loss of gloss, soiling, discoloring, edge wear, nicks, punctures, ink or foreign markings, peg hole punch, tape repair, focus, price sticker, sticker tear, sticker residue, water damage, bubbling and attached foreign objects.

对于包装背板，将由以下几个标准加以鉴定：摺痕，折痕，卷痕，撕裂，磨痕，划痕，翘起，机打标记，发乌，污点，脱色，边沿磨损，裂痕，穿孔，墨水痕迹或其它后添痕迹，挂装孔，图案模糊，价签，标签撕痕，标签粘痕，水泡痕迹，表面起泡，以及其它各种后添附着物。

Nanhauang-His stream and The Beihaung-His are located in the volcano function area, the stream has introduction of the sulphur gas and hot spring water of volcano, changes the environmental condition of the water body, influences the heavy metal and moves the course.There is a hot spring in area on the volcano after one The Shuang-His streams belongs to too, but the carbonic acid spring, there is less content of sulphate.Therefore, we can realize it was pH value that influenced the dissolving heavy metal to move mainly.

南磺溪与北磺溪的地区属后火山区域有喷气孔、硫气孔和温泉的存在，溪水有火山硫磺气体与温泉水的输入，改变水体的环境条件，影响到重金属迁移过程，双溪也属后火山区域有一处温泉，但属於碳酸泉，硫酸盐含量较少，由此我们可知，主要影响溶解性重金属迁移的是pH值。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。