后壳

By using the OP-10/JFC/ amyl alcohol non-ionic compound emulsifier as stabilizer, about 12% organosilicon was appended after the pre-polymerzation of MA, n-BMA, and MAA acrylate monomer was performed and the temperature was decreased to 62℃, and then the pH value was controlled in 7~8 after the polymerization.

结果表明，以OP-10聚醚渗透剂JFC/戊醇非离子复合乳化剂为稳定剂，在MA、n-BMA、MAA丙烯酸酯单体形成预聚体后降温至62℃，加入12%左右的有机硅单体，反应结束后控制pH值7~8，微乳液具有良好的稳定性，乳胶粒具有翻转型的核－壳结构。

The presence of early CT signs has been reported to be a predictive sign of hemorrhagic complications after reperfusion therapies[4, 5, 13 18]. Our study has also demonstrated that the rate of symptomatic hemorrhagic complications showed a tendency to increase as the grade of early CT signs increased, although a statically significant difference was not proved. A grade III CT sign may be a predictive sign for hemorrhagic complications after reperfusion therapy. In contrast, the presence of a grade I CT sign may not contraindicate reperfusion therapy.

本研究也显示：尽管彼此间均无统计学显著差异，但血管内再通治疗后症状性出血并发症的发生率，在深部大脑中动脉分布区未出现早期CT征象的患者、出现I级早期CT征象（细微低密度区局限于岛叶，基底节区正常）的患者、出现II级早期CT征象（部分壳核后外侧区出现低密度）的患者、出现III级早期CT征象（整个豆状核区均出现低密度）的患者中，呈逐渐增加的趋势。

And the last, the effects of fermented cottonseed-hull on dairy performance were studied with feed tests. In experiment 1, Cottonseed-hull and comminuted cottonseed-hull were fermented with complexing strains of microbials including EM and probiotics, using seven pairs of formulation have been formed by corn meal, molasses and fecula combined with urea and zein meal.

试验一利用EM菌液与益生素混合扩大培养后的复合菌液，选用玉米粉、糖蜜、淀粉三种炭源和尿素、玉米蛋白粉两种氮源共组成了7组发酵底物配方，将棉籽壳分成微粉样与原始样，发酵结束后对测定结果进行统计分析。

An amount increase ofPAI-3 andK10 was observed after theKCswere cultured for48 h in high calcium medium,butat72 h the exprssion ofPAI-3 decreased. The positive stain ofPAI-3 wasmainly localized in perinuclearmembrane in lowcalcium medium and in cytoplasm in high calcium medium. PAI-3 was incorporated into cornified envelope ofterminally differentiated keratinocytes.

结果 高Ca2+浓度下培养24 h后，分化标记物K10弱阳性表达， PAI-3呈阳性表达；培养48 h后， K10强阳性表达，而PAI-3的阳性表达明显增加， 72 h后，表达开始降低，在低Ca2+浓度下阳性染色主要位于细胞核膜周围而高Ca2+浓度下主要位于细胞质，同时， PAI-3存在终末分化KCs角质壳中。

Breed helps advance somebody's career: Can sow breed, but 3 months left and right sides can maintain to lose gemmiparous power namely only below normal temperature after the seed is collected, and fruit hard surface is solid, water penetration is poor, gemmiparous very not orderly, after appropriate uses wet sand bed to accumulate vernalization, the seed with seasonable budding winkle, with nutrient bag grow seedlings, grow 60-70 till Xiaomiao centimeter when, choice monsoon field planting at earthy and loose in fecund loam.

繁育栽培：可播种繁殖，但种子采后在常温下只能保持3个月左右即失去发芽力，且果壳坚硬，透水性差，发芽很不整洁，宜采用湿沙层积催芽后，及时挑出萌芽的种子，用营养袋育苗，直到小苗长到60-70厘米时，选择雨季定植于土质疏松肥沃的壤土中。

Secondary growth of silicalite-1-seeded industrial CuO-ZnO-Al2O3 in alkaline solution with silica and alumina source produced intergrown ZSM-5 zeolite shells. After the shells were changed into HZSM-5, we compared the catalytical performance of the core/shell composite catalyst with a non-core/shell structured composite catalyst with the same component.

铜锌铝催化剂（CuO-ZnO-Al2O3）是工业上制备二甲醚的良好催化剂，我们将其表面预先包覆纳米沸石silicalite-1晶种，水热环境下二次生长后吸收硅源、铝源后形成ZSM-5膜，转换成氢型后形成CuO-ZnO-Al2O3/HZSM-5的核/壳结构复合催化剂。

Carmichaeli leaves were studied after spraying salicylic acid 2 days before inoculation with Septoria aconite Bacc.

用5mmol/L的水杨酸对附子抗病品种和感病品种分别进行诱导，两天后接种乌头壳针孢，研究处理后附子叶片相关酶活性的变化，结果表明，SA诱导后能明显增强附子叶片内POD、PPO、PAL的活性；诱导接种处理比对照接种处理酶活性增加更为明显，同时抗病品种比感病品种酶活性更强，增加幅度更大，高酶活持续时间更长。

(1) Verification of the expressions of Cfos in caudate nucleus, putamen, globus pallidus, hippocamp, nuclei ventrolaterales thalami, parv ocellular reticular nucleus, dorso lateral magnocellular nucleus after rats subarachnoid hemorrhage are variation with different phases.(2) There are certain relationships between subarachnoid hemorrhage and different phases.

（1）大鼠蛛网膜下腔出血后不同时相Cfos在脑膜、脑皮质区及尾壳核、苍白球、海马、背侧丘脑腹后外侧核、小细胞网状核、背侧旁巨细胞核中都有表达且表达量随时相改变；（2）大鼠蛛网膜下腔出血后相关症状与不同时相Cfos在脑中表达之间存在着相关联系，具有规律性。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。