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The decomposition of δ-ferrite at 750°,850° and 950℃ in quenched 18/8/3/1Cr-Ni-Mo-Ti stainless steel specimens has been studied microscopically,thetransformation products being electrolytically extracted and identified by X-raydiffraction method.

应用金相法研究了18/8/3/1 Cr-Ni-Mo-Ti不锈钢经1300℃固溶处理后在950℃,850℃及750℃恒温分解初期金相组织的变化,并用电解分离及X射线衍射方法鉴定在不同恒温分解阶段δ-铁素体分解的产物。

The appearance of Licha black pig features long trunk, thin chest, commonly, the chest circumference less than trunk for over 15cm, over 8 pairs of mammillae, but the back of trunk is lack of embonpoint.

里岔黑猪从外形结构来看:体躯较长,胸部较浅,胸围比体长小15厘米以上,乳头一般8对以上,后躯稍欠丰满。

As the male gametophyte, pollen delivers sperm to the embryo sac for fertilization by pollen-tube polarized growth.

花粉作为种子植物的雄配子体,通过萌发后极性生长的花粉管将精细胞送到胚囊完成受精作用。

When the microspores were cultured in the medium with 16% sucrose for 3 days, and then added an equal volume of medium with 13% sucrose, the embryoid induction frequency was enhanced greatly, up to 3.7 times of that cultured all time in the medium with 13% sugar.

如果在16%蔗糖培养基中培养3天后,添加等体积的13%蔗糖培养基,能够大大提高胚状体的诱导频率,为仅用13%蔗糖培养基培养的3.7倍。

Under suspension culture without cytokines, the putative EG clonies could be induced to form simple embryoid bodies.

将消化分散的类EG细胞在无分化抑制因子的类EG培养基中重悬培养,3~4天后,可见部分细胞团形成简单的胚体。

ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子,在许多同种的真核生物有它的同源基因,这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关,而这个家族的蛋白质和DNA的结合能,各种ARID蛋白质的专一性尽相同,过大致上偏好於和AT-rich的序结合;我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因,分别是garid1和garid2,我们首先对於garid1做分析;将AU1标记接到gARID1转染形鞭毛虫,用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下,过其阳性染色和蛋白质表现有明显增加;EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合,经由突变序分析,也显示gARID1的结合序为AGATC和AATAAAATA,随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通过构建真核表达质粒pCMVCD ,限制性内切酶消化鉴定后,采用Lipofectamine 2000 脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stemcells , NSCs),G418 筛选阳性克隆,加入不同浓度的52氟胞嘧啶(52Flourocytosine , 52FC),MTT比色法测定NSCs的生存率。

Approximately one-third of newly synthesized proteins are transported to the lumen of the endoplasm...

大约1/3新合成的蛋白质被转运到内质网并在此进行转录后修饰,如折叠、低聚体化等。

Strain 031057 was isolated from sea sand, collected from the South Sea. It was a bacterium with endospore, not movable, belonged to G+.Relative inhibition concentration of the bioactivity produced by 031057 was 100 mg/mL corresponding to hygromycin B.

菌株031057所产抗生素粗品的效价:相当于潮霉素B的100mg/mL;经海洋微生物抗稻瘟菌(卸ricularia grLSea)活性菌株的筛选24 h发酵,菌体生长达最大密度,其最大生长密度为 3.68 X 10'Cfu/InL,而其产生活性物质是在sd后达到最大值。

So-called anatomical stems hae a slight proximal posterior bow to reproduce the contour of the femoral endosteum, thereby predetermining the rotational alignment of the implant.

所谓的解剖型股骨柄近端有一略微向后的弓形,以重现股骨内部的轮廓,从而预先固定了假体的旋转方向。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。