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Aim To observe the c-fos expression in the caudate-putman of 6-OHDA-lesioned rats after Apomorphine intraperitoneal injection and to investigate the inherent mechanism of it.

目的观察6-羟基多巴胺(6-OHDA)毁损黑质后,不同时间点腹腔注射阿朴吗啡大鼠纹状体c—fos表达情况,探讨其可能机制。

Results After the stimulation ,the rotating behaviour induced by apomorphine and improved distinctly, and DA content in the striatum increased (P<0.01), and Glu content reduced significantly ( P<0. 05).Content of the ASP had no change significantly.

结果 电刺激丘脑底核后,改善了PD大鼠的旋转行为,纹状体中多巴胺含量明显增加(P<0.01),谷氨酸含量明显减少(P<0.01),天门冬氨酸则无明显改变(P>0.05)。

These results suggest that the activate spleen-energy method can achieve appetitive purpose by not only affecting the gastrointestinal tract function in periphery, but also regulating the process of ingestion in brain. The basic research about ingestion indicate: one hand, after food enters the gastro-intestinal tract, the endocrine cell of the intestinal tract is activated and secrete many kinds of brains intestines peptide, the level of the brain intestines peptide in blood as the periphery signal spreads to the central nervous system regions that control digest and feeding behavior. On the other hand, it has been shown that dorsal parabranchial neurons, containing CCK-8S, extend fibers to the VMH and are involved in the inhibition of feeding .

关于摄食控制的基础研究表明,一方面食物进入胃肠道后,激活肠道的内分泌细胞,分泌多种脑肠肽如CCK-8S,这些脑肠肽在血中的水平作为外周信号可通过"肠—脑轴"传入中枢神经系统介导消化和摄食行为的部位,影响摄食中枢神经元的活动调节动物的摄食行为;另一方面中枢神经元可合成释放内源性的神经肽直接作用于摄食中枢神经元调节摄食,如中枢鳃旁体神经元的神经纤维就可延伸到达VMH,并且合成释放内源性的CCK-8S。

Condidiospore germination and appressorium formation were completed 2 days after inoculation, the mycelium grew slowly on the lenticel surface 10 days after inoculation.

接种后10d,菌丝体在皮孔表面缓慢生长。

While compared with the vocal pieces of other composers in the same period of time, with all-sided analysis from the topic, the structure, the melody and the music element of S.C.Foster's vocal pieces, the article clearly shows the most prominent character of his songs —"approachability" and the important value of Foster in the development of American music and the history of human being.

本文通过对福斯特歌曲作品的创作题材、形态结构、乐汇来源等多角度的分析与阐释,使读者对福斯特歌曲作品在同时代其他声乐作品中体现出的"亲和力"的这一特征有一个全方位的、较为清晰的认识;并在对这些作品进行细致分析研究后指出,福斯特及其歌曲作品不仅在美国本土音乐文化发展史上有着重要历史地位,还因为歌曲作品本身所具备的对时空的超越性,即歌曲中饱满蕴含的人性和爱这一人类永恒的主题,而成为全人类共有的精神财富。

METHODS: The skinned first class arborization of superior mesenteric artery from HS rats were adopted to observe the influence of inhibitor of ZIPK on the effects of PKCα and PKCε agonists on calcium sensitivity after shock via measuring the contraction initiated by Ca2+ with isolated organ perfusion system, hypoxic vascualr smooth muscle cells were adopted to measure the protein expression and activity of ZIPK after applying PKCα and PKCε agonists following hypoxia via Western blotting.

采用离体微血管环张力测定技术,观察褪膜后抑制ZIPK对PKCα、PKCε激动剂增高失血性休克肠系膜上动脉一级分支微血管钙敏感性作用的影响;同时观察缺氧血管平滑肌细胞在PKCα、PKCε激动剂作用下ZIPK蛋白表达、活性的变化。

One of two sperm get into an archegonium and fused with egg. The nucleus of fertilized egg began to divide, and the walls were formed after the third division. The eggs without fertilization divided two times and then disappeared.

雄配子体产生的两个精子中的一个进入颈卵器与卵细胞结合形成受精卵,受精卵核到第3次分裂形成细胞壁,没有受精的卵细胞还要进行两次有丝分裂后才消失。

Different DNA constructs were introduced into the decapsulated cysts of Artemia by electroporation under 2KV, 20 μsec pulse length, 20 number of pulses and a DNA concentration of 20μg/μl in a volume of 100μl.

不同的构筑质体经由电穿孔法,被导入经去壳处理后的丰年虾休眠卵中,在2千伏特、20微秒的脉冲波长度、20次的脉冲数和在100微升的体积中DNA浓度为每微升20微克的设定条件下。

The constructed pcDNA3-IL-15 plasmid was identified by Bgl Ⅱ digestion,PCR amplification and sequenceanalysis respectively,to ensure that the hIL-15 cDNA was properly inserted into thevector and the sequence was correct.Then the pcDNA3-IL-15 plasmid was instantlyexpressed in COS-7 cells by means of lipofectamine transfection,and followed by astable expression in Chinese hamster oval cells.8 positive cell colonies wereobtained after the G418 selection,designated CHOA~H cells.

经过BglⅡ酶解分析,PCR扩增鉴定和DNA测序分析,证明IL-15 cDNA已正确插入克隆载体且序列正确后,采用脂质体法,首先将pcDNA3-IL-15重组质粒转染至非洲绿猴肾细胞(COS-7)做瞬时表达,再转染至中国仓鼠卵巢细胞进行稳定表达。

Methods: Audition, acoustic impedance and pure tone audiometer were measured in 32 cases after adenoidectomy.

对32例行腺样体切除后患儿进行听力、声阻抗、纯音测听方面的检查。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。