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We found the relationship among starch metabolism, metabolisms of other components and gene expressing profiling due to the accumulation of sugar. Then, genes expression patterns of starch biosynthesis in leaves and developing endosperms of 5 maize inbreds were studied for the contribution of different genes to starch biosynthesis in endosperm.

为深入了解玉米淀粉合成的分子调控机理,本研究首先以B73、ae/wx和sh1授粉后15天的胚乳为材料,利用18K Affymetrix玉米基因组芯片平台,研究了突变体材料中基因表达谱的变化,发现了淀粉合成受阻后糖的积累对其它物质代谢和基因表达调控的影响;在此基础上,对可能参与淀粉合成的基因以多个自交系为材料,分析它们在叶片和胚乳发育过程中的表达模式,研究了不同成员对胚乳淀粉合成的贡献。

Before transfected with AT1, neither AngⅡ nor stretch increased the activation of ERKs. After transfected with AT1, pretreatment with AngⅡ for 8 min or stretch for 10 min activated ERKs obviously.

COS7细胞转染AT1受体以前, AngⅡ和牵张刺激都不能引起细胞内ERKs磷酸化升高;而转染野生型AT1后,AngⅡ和牵张刺激引起细胞内ERKs磷酸化明显升高,各突变体中,Q257A和C289A转染细胞后细胞对牵张刺激的反应受到明显抑制,提示AT1的牵张感受位点在Q257A和C289A。

ES cells were maintained on gelatin-coated dishes in Iscove's modified Dulbecco medium containing 0.1 mmol/L of nonessential amino acids, 2 mmol/L of L-glutamine, 0.1 mmol/L of monothioglycerol, 15% fetal bovine serum, and 1000 U/mL leukemia inhibitory factor. Embryonic bodies were firstly formed by ES cells with hang drip method in IMDM without LIF. Six-day-old EBs were trypsinized and the cells were resuspended at a concentration of (3-4)×109 L-1 cells per 1 mL of a 1:1 mixture of culture medium and Matrigel?

实验方法:将鼠胚胎干细胞株接种于被覆明胶的培养瓶内,加入含0.1 mmol/L非必需氨基酸、2 mmol/L L-谷酰胺、0.1 mmol/L二羟基丙硫醇、15%胎牛血清、1 000 U/mL白血病抑制因子的IMDM培养液。3 d后采用&悬滴法&使细胞在不含白血病抑制因子的上述IMDM培养液中形成胚胎体,6 d后胰酶消化得到胚胎干细胞,浓度调整为(3~4)×109 L-1,与Matrigel?

MAIN OUTCOME MEASURES: One week later, percentage of rat peripheral blood leucocytes and mononuclear cells was counted in each group. Heart function was compared among groups 4 weeks later.

主要观察指标:1周后计数3组大鼠外周血白细胞及单个核细胞百分比,4周后比较3组大鼠在体心功能。

Our investigation has shown that:ⅰ there was an interference effect between the incident and reflected matter waves as all the atoms in BECs were coherent;ⅱ considering the rotation of a vortex, the nonuniform velocity distribution along the surface would play an important role in the quantum reflection process. For example, they would change the characteristics of the interference fringes between the incident and reflected matter waves;ⅲ the reflected vortex became unstable for sufficiently low incident velocity or strong interaction. This sort of instability physically originated from dynamics excitations induced by the atomic interaction in the quantum reflection process.

我们的研究表明:ⅰ由于相干性质的存在,在涡旋与固体表面相互作用的过程中,被固体表面反射回去的部分与正在入射的部分在空间叠加后一定会产生清晰的干涉条纹;ⅱ由于各向异性的速度分布,涡旋的反射率也呈各向异性,因此反射回来的部分与入射部分形成的干涉条纹宽度以及疏密程度与入射速度以及速度各向异性的程度有关;ⅲ由于玻色凝聚体中原子间相互作用的存在,反射过程中凝聚体会产生激发,破坏涡旋的结构,使得反射后的涡旋不再是中心对称。

Li lines related to Di-center, which can be observed in all SiC polytypes after various kinds of particle bombardments or irradiations and can withs

在700℃温度退火后的样品中观察到D_I-center,该中心在幅照或粒子轰击后的SiC的各种多型体中都观察到,并能经受高达1700℃温度的退火。

Cu-Ni sulfided mine complex locate in BaimaZai of JinPing County is sub alkali iron-super iron, it"s zoning is clear and it"s Ni-Cu-Co melting first and enrichment, its rare earth is rich in right lean light rare earth, but Eu in olive rock is serious to be bad, Gd-Tb is tittles negative abnormity. Above-mentioned show that the mineral is cone from mantle, and didn t mix with crustal substance, and continent tholeiite magma turning into continent tholeiite emplacement after by surging.

金平白马寨铜镍硫化矿床杂岩体分带明显、属亚碱性铁质-超铁质岩、具Ni-Cu-Co三元素先熔离,后富集的规律、稀土配分为右倾斜轻稀土富集型,但橄榄岩铕亏损显著,Gd-Tb微小负异常,显示成矿物质来源地幔,与地壳物质无混染和大陆拉斑玄武岩浆上涌后变为大洋拉斑玄武岩浆侵位特点。

The results show that in the applied load range of 10250 N, the mass loss rate of coated AZ91D alloy can be identified to three stages, which are related with whether the coating is worn out. Abrasion is the main mechanism for the coated alloy. The friction coefficient of the coated alloy during sliding wear appears oscillation before it reaches a constant value, which is close to the μ value of the matrix alloy. The mass loss rate of coated alloy increases slightly with increasing applied load when the applied load is more than 150 N because of high plastic deformation accommodation on the matrix alloy surface.

结果表明:根据载荷大小,涂层处理后AZ91D镁合金的磨损行为明显分为3个阶段,这些阶段同陶瓷层是否磨穿密切相关;微弧氧化涂层AZ91D镁合金的磨损机理主要是磨粒磨损;受表面状况的影响,陶瓷涂层的摩擦因数在 0.20~0.45 间波动;在较高载荷下,涂层被磨穿后,涂层的摩擦因数趋于基体合金的摩擦因数,涂层合金的表面变形能力提高,该阶段质量磨损速率随载荷增加,趋势变得缓慢。

It is showed that MG132 significantly reduced the germination rate and tube growth. Furthermore, MG132 treatment lead to vacuolization occurred both in tube cytoplasm and generative cell. While DMSO and non-proteasome inhibitor E-64 do not have similar effects. FTIR analysis revealed that MG132 treatment markedly reduced the contents of wall-bound proteins and pectin at the apex of tube.

结果表明:MG132显著抑制青扦花粉萌发和花粉管生长,并导致花粉管形态异常,主要表现为花粉管亚顶端出现液泡化,并且液泡随着培养时间的延长而扩大到整个花粉管,花粉管濒临死亡;而DMSO以及非蛋白酶体抑制剂E-64不产生类似结果;半薄切片结果表明,MG132处理后不仅花粉管细胞质发生液泡化,生殖细胞也发生液泡化;FTIR分析进一步表明,MG132处理后,花粉管顶端的细胞壁蛋白和果胶质含量大幅度下降。

Second,we set the database of morphological characteristics of intercepted and captured bark beetle during quarantine,and the last,we studied the molecular identification of 15 species.The results were showed as below.1.Application of bark beetle semiochemicals in timber quarantine1.1 EAG and walking responses of bark beetle to different semiochemicalsSemiochemicals which can induce the olfactory response and aggregation behavior of bark beetle include host volatile components,pheromones secreted by bark beetles itself or synthesized with host compounds by bark beetles,and the compounds released by tissue of decaying wood under the pressure of microorganism after bark beetle colonization.

信息化合物在小蠹虫检疫中的应用1.1小蠹虫对各种信息化合物的触角电位及嗅觉反应能诱发小蠹虫产生嗅觉反应,进而使之产生聚集行为的信息化合物有很多种,主要包括:树木的挥发性组分;小蠹虫自身产生的信息素和寄生后转化寄主化学物质合成的信息素统称虫体信息素以及树木被小蠹虫寄生后,其组织在微生物作用下释放的物质。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。