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Bone marrow-derived mesenchymal stem cells are capable of chondrogenesis, making them a possible source of cells for injectable cartilage tissue engineering. There exist different ideas on the ability of mesenchymal stem cells's chondrogenesis in monolayer culture. Because of this, the effect of adult rabbit's bone marrow-derived mesenchymal stem cells chondrogenesis in monolayer culture was studied. The mesenchymal stem cells was isolated from adult rabbit's bone marrow and monolayer cultured. TGF-β1, Vit-C and Dexamethasone were used. Immunohistochemistry analyses and histological staining of H-E, Methylaniline blue and Alcian blue were performed to identify the expression of collagen type Ⅱ and cartilage associated matrix. The results showed that the induced cells expressed and produced collagen type Ⅱ and cartilage associated matrix. This suggests that the differentiation of adult rabbit's marrow-derived mesenchymal stem cells into chondrocyte in monolayer culture is feasible and may be induced by TGF-β1, Vit-C and Dexamethasone.

骨髓基质干细胞的软骨分化潜能使其可能成为可注射组织工程化软骨研究的种子细胞,为探讨体外培养的骨髓基质干细胞在平面诱导条件下软骨分化的可行性,我们进行下列实验:获取并体外平面培养成体兔骨髓基质干细胞,应用TGF-β〓、Vit-C和地塞米松对其软骨分化诱导,诱导后的骨髓基质干细胞行细胞爬片组织学和Ⅱ型胶原免疫组化,结果证实,诱导后骨髓基质干细胞可分泌Ⅱ型胶原,组织学染色可见类似于软骨细胞,由此证明体外培养的骨髓基质干细胞在平面诱导条件下可以软骨分化,其软骨诱导因子为TGF-β〓、Vit-C和地塞米松。

The results showed that:(1) Many proliferating cells were distributed unevenly in the ventricular zone of telencephalon, and the [3H] labeled cells were found to be populated in the lateral ventricular zone of neostriatum at brain level L12~L14,forming proliferation "hot spots";(2) Having underwent mitosis twice, the proliferative cells in the VZ began to migrate laterally 6 days after intramuscular injection of [3H] thymidine, and these migrating precursor cells terminated at their targets and differentiated into new neurons on the 30th day.

结果发现:①端脑室带区中存在不均匀分布的[3H]标记细胞,大量标记细胞分布在端脑尾侧新纹状体水平(L1~L14)的VZ,形成标记细胞的&热点&区;②标记细胞经两次有丝分裂后,大约在肌肉注射[3H]的第6天后开始从VZ向外迁移,在30天左右到达目的脑区并分化成为成熟神经元。

The therapy groups displayed significant improvements in neuromotor functions and survivals of neuron and oligodendrocyte compared with control groups. These results demonstrated the neuroprotect roles of GGF2, and cationic liposome-mediated GGF2 transgene therapy has potential possibility.

结果:1、大鼠LFP致伤后3—7天,GGF2 mRNA在海马CAl区、CA2区、CA3区、DG及皮层表达明显增加。2、阳离子脂质体介导GGF2转基因治疗可促进大鼠伤后行为学的恢复,可促进损伤区神经元及少突胶质细胞的存活。3、原核表达方法得到GGF2重组蛋白。

Objective To probe the possibility of functional sensory endings regeneration after end-to-side neurorrhaphy. Methods Fifteen New Zealand rabbits were used in this study.

摘 要:目的探讨感觉神经端侧吻合后供神经的轴突长入神经移植体后,最终能否形成具有功能的感觉末梢。

After active infection of HCMV, in adult NLBP patients, there were nontypical HCMV inclusion bodies in HE dyeing uropsammus smears under light microscope.

成年人HCMV活动性感染后导致的NLBP患者,尿沉渣涂片经HE染色后光镜下见不典型HCMV包涵体。

The coarse needle-like or nubby Al9FeNi phase existed in the cast microstructure of high content Fe and Ni alloy 2618(2#). After hot extrusion, it was broken into coarse multi-angle Al9FeNi particles. The contents of Fe and Ni have no effect on aging characteristic and the hardness of the alloy after aging.

含锆的高铁、镍2618合金经熔体高温过热处理后(4#),在合金铸态组织中获得均匀细小的针片状Al_9FeNi相,热挤压后获得均匀细小的颗粒Al_9FeNi相;弥散Al_3Zr粒子的存在可使2618合金得到细小的再结晶晶粒组织,该合金既可获得较高的高温强度,还可获得较高的延伸率。

After injection of FG into the NTS, FG retrogradely labeled neurons were present bilaterally with a strong ipsilateral predominance in the hypothalamic regions, such as medial and lateral parvicellular portion of the paraventricular nucleus, posterior

将FG注入孤束核后,在下丘脑室旁核小细胞部、穹窿周区、下丘脑后区及弓状核等核团内都观察到数量较多的FG逆行标记神经元;EM1和EM2阳性神经元的胞体主要位于下丘脑背内侧核、腹内侧核

The results showed that the lower agglutination titers were observed at day 7 and up to higher levels over 〓 or 〓 at day 21 induced by the vaccine of recombinant pilin and the recombinant fused protein respectively. Above results suggested that the good immunogenicity of the two recombinant vaccines could match with both the D.

结果纤毛蛋白基因工程疫苗在免疫7天后,被免兔血清中出现抗E型节瘤拟杆菌凝集抗体,21天后被免兔的血清凝集效价达到〓,与节瘤拟杆菌菌体灭活疫苗,尤其与纤毛蛋白基因工程疫苗对照免疫试验相一致。

Enhanced awareness of dangers and rational treatment will help reduce the pulmonary sudden death. Drug-induced pneumonosis ; Sudden death ; Systematic review ; ADR

案例选择标准如下:①用药前疾病诊断明确,排除由于漏、误诊而导致病情骤变死亡的案例;②病情突然变化和死亡与用药有直接关系;③死亡的时间限定在用药后 2 4h内;④病人猝死前的症状、体征、临床表现或死亡后的尸体解剖以

The results were:The main morphological difference were that the color of squama, median fascia, reniform stigma, orbicular stigma, the part between postmedian fascia and subterminal fascial and terminal hair on forewings, hindwings, terminal hair on hindwings, and the color of skin under squama on obdomen were black, and the color of pupa was also black, and the color of hair on dorsal thorax and obdomen were white.

结果表明: JBM和JBW的主要形态区别表现在成虫前翅上鳞片、中横线、肾状纹、环状纹、外横线及亚外缘线之间的部分、前翅缘毛、后翅、后翅缘毛、腹部鳞毛下的皮肤颜色以及蛹体色等发生了黑化,成虫胸背毛颜色以及腹部毛颜色发生&白化&。

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The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为:以确有疗效的中药复方为研究对象,以现代科学技术和传统中医药理论为技术支持,以该复方所治病证的药效响应为评价标准,以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。

I don't… don't know. He's unconscious.

我不……我不知道他休克了。