后体

The differentiation of the cells and the expression of Myosin Ⅶa were revealed by immunohistochemical staining.

结果脂质体介导的基因可在细胞内获得良好的表达，转染后24h，相差显微镜下观察细胞形态发生变化，免疫组织化学显示转染后细胞可以表达毛细胞特异性蛋白Myosin7a。

The biological activities of the metal complexes of derivatives of benzaldehyde nitrogen mustard were investigated against 〓,〓 and SMMC-7721 cell lines. The interesting results were found. When the non-activity ligand combines with cobalt and nickel , the complexes have good antitumor activity, while active ligand binds to the metal, the complex has no activity, indicating there are synergic effect and counteraction between the metals and the ligands.

对所获得苯甲醛氮芥衍生物的金属配合物进行研究，观察它们对SMMC-7721、〓细胞株的作用，发现某些有趣的实验结果，无活性的配体，当与钴，镍形成配合物后，具有很好的抗肿瘤活性，而有活性的配体形成配合物后无活性，揭示在它们存在有协同作用及拮抗作用。

The conclusions were as follows: theνO-H andνC=O IR absorption maxima shifted towards higher wave numbers after imprinting 17β-estradiol on TFMAA-co-TRIM copolymer with the red shift of theνO-H groups being apparent after precipitation polymerization for 16h while that forνC=O groups being observed after polymerization for 24h. A strong interaction between TFMAA and 17β-estradiol was confirmed by the high selectivity for 17β-estradiol, as indicted by the values of the separation factor of isomers of 17β-estradiol/17α-estradiol (2.28) and the imprinted factor (3.01). Particle diameter of TFMAA-co-TRIM polymer was between 300 nm and 1.5μm, which suited well for solid phase sorbent throughout at low column pressure. The recognition of imprinting TFMAA-co-TRIM polymer for sterol molecules was driven by enthalpy eluted with acetronitile, and low temperature was in favor of the separation of sterol structure analogues on imprinting column. TFMAA-co-TRIM polymer possessed of determinate anti-heat stability, with melting point beginning at 255.84℃, Tp=257.40℃, control TFMAA-co-TRIM polymer and imprinting TFMAA-co-TRIM polymer were respectively decompounded at 267.79℃and 343.11℃, and solid micro-extraction noddle prepared by the special polymerization also showed definite recognition for 17β-estradiol by GC/MS detecting at 270℃. By selection of various washing and elution solvents, elution reagents of close polymerization system were of more advantage in template molecules retention and recognition on molecularly imprinted solid phase extraction column. At a certain extent, progesterone, 17α-estradiol, 17β-estradiol and 4-androstene-3, 17-dione could be intercepted on the MISPE column. Especially, MISPE had high selectivity for progesterone, and imprinting TFMAA-co-TRIM polymer could achieve adsorption balance within 50 min by absorption kinetics test for 17β-estradiol. However, MISPE column showed better selectivity and enrichment property for 17β-estradiol than C18 and CSPE columns according to the data from HPLC and GC/MS analyses. Recovery of 17β-estradiol on MISPE column was up to 85.5% while when prime extracting solution of milk powder was sampled, the recovery of CSPE and C18 columns were 43.7% and 30.7%, respectively.

通过研究阐明：紫外聚合产物TFMAA-co-TRIM中的νO-H振动吸收峰在聚合16h后红移，νC=O振动吸收峰在聚合24h后红移；TFMAA-co-TRIM对雌二醇异构体的印迹因子达到了3.01，α为2.28，优于其它功能单体参与得到的聚合物识别特性；TFMAA-co-TRIM聚合物粒径介于300 nm至1.5μm之间，作为色谱固定相具有良好的通量和低的柱压；在乙腈流动相中，TFMAA-co-TRIM分子印迹固定相的分离过程主要被焓驱动，低温有利于分子印迹固定相分离甾醇结构类似物；TFMAA-co-TRIM分子印迹聚合物在255.84℃时开始熔融，Tp=257.40℃，聚合物CP的降解温度在267.79℃，MIP降解温度在343.11℃，制备的固相微萃取头初步经GC/MS 270℃的耐热性测定；不同的洗提溶剂筛选证明了选择接近聚合溶剂的洗脱体系更有利于MISPE发挥识别效应；分子印迹固相萃取柱对孕酮、17α-雌二醇、17β-雌二醇和雄烯二酮都具有不同程度的保留特性，可作为此类化合物的吸附材料，特别对孕酮强保留的富集特性，可作为孕酮的选择性识别吸附剂，其分子印迹聚合物对17β-雌二醇的吸附动力学测定显示在50 min内基本达到吸附平衡，具有作为传感器核心敏感材料的潜力；对比萃取奶粉中17β-雌二醇性能，MISPE柱比非分子印迹固相萃取柱和C18柱具有更高的保留，回收率依次为85.5%，43.7%和30.7%。

Lawrence martyr , 10 august : the monthly recurrence known as the new moon with the old moon in her arms : the posited influence of celestial on human bodies : the appearance of a star 1st magnitude of exceeding brilliancy dominating by night and day a new luminous sun generated by the collision and amalgamation in incandescence of two nonluminous exsuns about the period of the birth of william shakespeare over delta in the recumbent neversetting constellation of cassiopeia and of a star 2nd magnitude of similar origin but lesser brilliancy which had appeared in and disappeared from the constellation of the corona septentrionalis about the period of the birth of leopold bloom and of other stars of presumably similar origin which had effectively or presumably appeared in and disappeared from the constellation of andromeda about the period of the birth of stephen dedalus , and in and from the constellation of auriga some years after the birth and death of rudolph bloom , junior , and in and from other constellations some years before or after the birth or death of other persons : the attendant phenomena of eclipses , solar and lunar , from immersion to emersion , abatement of wind , transit of shadow , taciturnity of winged creatures , emergence of nocturnal or crepuscular animals , persistence of infernal light , obscurity of terrestrial waters , pallor of human beings

显示出不同程度之生命力的缤纷色彩白浅黄深红朱红银朱诸星之亮度一直包括到七等星以等级标志的诸星之大小诸星的位置御夫座沃尔辛厄姆路172大卫的战车173土星光环螺旋星云凝固后形成有卫星的恒星群两重大阳相互依存的旋转运动伽利略西蒙马里乌斯174皮亚175勒威耶赫歇耳加勒176等人各自独立地同时所做的发现波得和开普勒所尝试的距离的立方与回转次数的平方的体系化177多毛的众彗星178那几殆无限的被压缩性，以及自近日点至远日点那广漠的远心的重返大气层的椭圆轨道陨石的恒星之起源年纪较轻的天体观测者诞生的那个时期火星上所出现的"暗波"现象179每年在圣劳伦斯节殉教者，八月十日前后降落的陨石雨每月都发生的所谓"新月抱旧月"现象180关于天体对人体的影响的假定威廉莎士比亚出生的时期，在斜倚却永不没落的仙后座那三角形上端，一颗不分昼夜散发着极亮光彩的星辰一等星出现了181这是两个无光死灭了的太阳因相撞并汞合为白热体而形成的灿烂的新太阳大约在利奥波德布卢姆出生时，出现在七星花冠星座里而后又消失了的一颗同一起源亮度却稍逊的星宿二等星182还有约于斯蒂芬迪达勒斯出生时，出现在仙女座中之后又消失，小鲁道尔夫布卢姆出生与夭折数年后出现于御夫座后又消失，以及另外一些人出生或去世前前后后出现在许许多多其他星座中而又消失了的假定是同一起源的实际存在或假定存在的星斗183 。

Preferential oxidation of Ti on NiTi alloy surface has been observed and was consistent with the experimental result and the free energy of formation A G?(298K) of NiO, TiO, TiO2. Mulliken population and the partial density of state analysis showed that the interaction between Ti and O atoms was mainly donated by O 2p and Ti 4s orbital and O 2p and Ti 3d, 4s, 4p hybridization orbital. The electron structure in NiTi bulk material was not altered during 62 molecule adsorption process and the surface passivation oxide formation.

进一步对钛与氧之间相互作用本质的研究表明，O 2p轨道和Ti 4s轨道以及O 2p轨道和Ti 3d 4s 4p杂化轨道间的相互作用是NiTi合金表面氧分子吸附的驱动力，在此过程中，NiTi合金的体相结构没有发生变化，从而保证了其一系列力学性能和形状记忆特性在表面氧化后不发生明显的变化，使表面改性的工作可以安全、可靠的完成而不影响其植入人体后发挥正常的生理功能。

Mulliken population and the partial density of state analysis showed that the interaction between Ti and O atoms was mainly donated by O 2p and Ti 4s orbital and O 2p and Ti 3d, 4s, 4p hybridization orbital. The electron structure in NiTi bulk material was not altered during O〓 molecule adsorption process and the surface passivation oxide formation. Thus the mechanical properties and shape memory effect would not change after NiTi alloy surface oxidation. Stable microstructure demonstrates reliability of implant material.

进一步对钛与氧之间相互作用本质的研究表明，O 2p轨道和Ti 4s轨道以及O 2p轨道和Ti 3d 4s 4p杂化轨道间的相互作用是NiTi合金表面氧分子吸附的驱动力，在此过程中，NiTi合金的体相结构没有发生变化，从而保证了其一系列力学性能和形状记忆特性在表面氧化后不发生明显的变化，使表面改性的工作可以安全、可靠的完成而不影响其植入人体后发挥正常的生理功能。

After full dilation of ostium of uterus 100 ml 5 % glucose plus 10 ml 55 calcium gluconate injection was administered intravenously , which was finished in 25 minutes.

方法在宫口开全后观察组静脉滴注5％葡萄糖液100ml加10％葡萄糖酸钙10ml，25min内滴完；两组均在胎儿娩出后宫体注射缩宫素20U。

The movement of rats' palp began at day 10.4±1.3 and the movement of rats paip approached to normal at day 18.5±1.3 in CDNF gene transfection group.

面神经损伤后经脂质体介导GDNF基因体内转染靶器官后，对受损面神经的修复有促进作用。

Fluorescence in situ hybridization was carried out to confirm the integration of HBV DNA into male pronucleus and its replication with cell division in embryonic development.(Reverse transcriptase polymerase chain reaction,RT-PCR) and immunofluoresence assay were performed to observe the expression of the HBV gene in two-cell stage.

材料与方法：成熟雄鼠麻醉后双侧睾丸注射经脂质体DOSPER包裹的HBV质粒，手术后雄鼠与超排雌鼠合笼交配，用荧光原位杂变(Fluorescence in situ hybridization，FISH)分别检测单细胞胚和二细胞胚间期核中HBV DNA的存在与复制，用逆转录聚含酶链反应(Reverse transcriptase-polymerase chain reaction，RT-PCR)和免疫荧光方法检测HBV基因在二细胞胚胎中的表达。

Next, BALB/c mice were orally infected with Y. pseudotuberculosis and its ompR mutant to study metabonomic variation of different intestine-associated organs after 24 h infection. The results showed that a majority of metabolites were decreased during this acid adaptation process, but phosphoenolpyruvate, 5'-GMP, 5'-AMP, 5'-UMP, GDP/GTP of wild type strain and lactate, Tyr, phosphoenolpyruvate, 5'-AMP、GDP/GTP of the ompR mutant strain were increased.

为了探讨病原细菌感染动物后特别是在酸性环境下代谢物的变化规律，首先在体外模拟假结核耶尔森氏菌及其全局性调控因子OmpR突变株在适应酸性环境时所引起的菌体自身的代谢变化，然后通过BALB/c小鼠口服感染耶尔森氏菌，研究小鼠感染病原细菌24h后各种相关器官的代谢变化。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。