后体

OBJECTIVE: To compare the clinical outcome between fixed platform high-flexion prosthesis and rotating platform high-flexion prosthesis following total knee arthroplasty.

目的：比较应用固定平台高屈曲假体与旋转平台高屈曲假体行全膝关节置换后临床疗效的差异。

In fact, from Wahaha management systems and practice, we can see that the management strategy: First, to implement a win-win aggregators institutional, Wahaha in China's 31 provinces to strictly selected more than 1,000 dealers, formed covering nearly Every town in China a joint marketing system, and use margin approach to building confidence between the parties, this approach dramatically changed the Wahaha trade forms of organization, Wahaha's chairman and general manager Zong said that this organizational form of "aggregators body"; Second, the implementation of differential pricing system, Wahaha's existing sales network of the company itself, a special dealer, special two dealers, secondary dealers, three dealers, retail terminals, and strict price control system; 3, comprehensive incentives, Wahaha's rebate is not a single incentive sales rebate of such direct incentive, but rather to include a variety of promotions and other indirect incentives, including a comprehensive incentive measures; 5, strict accountability and developed a regional strict reward and punishment system, Wahaha and dealer signed a contract to the authorized dealers to strictly limit the sale of the area to prevent the dealer cross-regional sales in order to strictly protect the dealers around the interests; 6, and distributors to establish deep feelings, Wahaha and distributors Once the partnership working to develop a long-term strategic partner; 7, emphasis on marketing, team building, Wahaha throughout the country only more than 3000 sales staff Why so few sales staff can help the company to complete more than 32.5 billion yuan in annual sales, which Wahaha focus on team-building and marketing are inseparable.

其实，从娃哈哈的管理制度和实际操作中我们可以发现其管理策略：一、实行双赢的联销体制度，娃哈哈在全国31个省市严格选择了1000多家经销商，组成了几乎覆盖中国每一个乡镇的联合销售体系，并采用保证金的方式建立双方的信任，这种方式极大地改变了娃哈哈的交易组织形式，娃哈哈公司董事长兼总经理宗庆后称这种组织形式为"联销体"；二、实行级差价格体系，娃哈哈现在的销售网络构成是公司自身、特约一级经销商、特约二级经销商、二级经销商、三级经销商、零售终端，并实行严格的价格管理体系；三、全面的激励措施，娃哈哈的返利激励并不是单一的销量返利这样的直接激励，而是采取包括各种优惠活动等间接激励在内的全面激励措施；五、实行严格的区域责任制并制定了严明的奖罚制度，娃哈哈和经销商签订的合同中给特约经销商限定了严格的销售区域，防止经销商跨区域销售，以严格保护各地经销商的利益；六、与经销商建立深厚的感情，娃哈哈和经销商一旦确定合作关系就致力于发展成为长期的战略伙伴；七、重视营销队伍的建设，娃哈哈在全国各地只有3000多销售人员，为什么如此少的销售人员可以帮助公司完成超过325亿元的年销售额，这与娃哈哈注重营销队伍的建设和培养是分不开的。

As a result, Core gene was expressed in E.coli with yields of 20% of total proteins and it was a 31KD fusion protein on SDS-PAGE and Western-Blot, it shows that Core protein was highly expressed in E.coli;One fragment of 369bp could be seen on the gel of the Core gene PCR product. A band of 29KD could be seen on the Western-blot after induction of yeast containing pPICZaA-Core for 72 hours. This indicate that Core protein was secretively expressed in the Pichia pastoris with weak yields.

结果显示pBVIL1-Core的表达产物经SDS-PAGE分析出现一条约31KD的带，与预期融合蛋白的分子量相符，表达蛋白存在于包涵体中且表达量占菌体总蛋白的20%，Western-blot显示诱导后菌体在相应位置出现特异性杂交带，表明

Chromosome pairing behavior in meiosis of the hybrid F1 was carried out. Results showed that in an average , 1.25 rod bivalents were observed in one PMC, meiotic configuration was 2n=14=11.49Ⅰ+1.25Ⅱ(Xta=1.25) and most of PMCs possessed 1～5 bivalens, indicating that the relatively high homeology was detected between the D genome of Ae.tauschii and the V genome of D.villosum. The morphological differences between F1 hybrids and their parents were significant. F1 plants were highly self-sterile, but partially self-fertile after treated by chromosome doubling technique.

对杂种F1花粉母细胞减数分裂中期Ⅰ染色体配对行为进行观察发现，"节节麦×簇毛麦"杂种F1平均每PMC有1.25个棒状二价体，染色体的平均构型为2n=14=11.49Ⅰ+1.25Ⅱ(Xta=1.25)，大部分被观察的细胞出现1～5个二价体，表明节节麦D染色体与簇毛麦V染色体间具有相对较高的部分同源配对， D和V染色体之间存在一定的部分同源性。F1植株高度自交不育，经染色体加倍处理后能够自交结实。

The upper volume lies over it, by abstraction it differs from the stone socle and the terrain.

上部体块部分悬挑在下部体块之外，抽象处理后的形态，与石基和地形形成鲜明对比。

Using RT-PCR analysis, RGS7 was expressed from fertilization to 96 hours post-fertilization of Zebrafish. Expression of RGS7 was found in brain、aorta ventralis、intermediate cell mass and somite by whole-mount in situ hybridization.

因此，利用RT-PCR以及全胚体原位杂合技术，结果发现在受精后0~96小时的斑马鱼胚胎中皆可侦测到RGS7的表现，其表现的位置是在斑马鱼胚胎的脑部、腹侧主动脉、中期细胞团及体节等组织部位。

Because of the significance of zebrafish Dapper2 in mesoderm induction, the mouse dapper2 was cloned following its homolog. The study of its spatial and temporal expression pattern showed that it expressed specifically in otic vesicle, somite,gut and roof plate of mouse embryos.

鉴于Dapper2对于胚胎中胚层发育的重要性，本文首先克隆出小鼠Dapper2基因，并研究其时空表达谱，发现它在体节形成期后在体节、耳泡、神经顶板、肠等组织中特异性表达。

In 10.5 day mouse embryo, by the induction of notochord, mesenchymal cells of ventromedial somite differentiate into sclerotome, cells migrate towards notochord, forming perinotochord tube around notochord, the cartilage is till invisible, alcian dying is faintly positive.

椎体区域发生骨化后（E16.5），阿利辛兰染色呈阴性，茜素红染色呈阳性，仅有Ihh在椎体边缘有表达，无PTHrP表达；在椎间盘区域，仍然保持阿利辛兰染色阳性，仅有PTHrP的表达，无Ihh表达。

Quenching and tempering treatment to be tempered sorbite organization, and its mechanical properties than those of equal hardness and normalizing sorbite organizations as excellent.

调质处理后得到回火索氏体组织，它的机械性能均比相同硬度的正火索氏体组织为优。

It is found that the fine tempering sorbite is obtained in condition of 930℃ direct quenching temperature and 640℃ tempering temperature after controlled rolling.

结果表明：经奥氏体再结晶区和未再结晶区两阶段结合控制轧制后在线930℃直接淬火、640℃回火，钢板可获得均匀、细小的回火索氏体组织，其综合力学性能均超过标准要求。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。