后体

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

The changing trend of progesterone during oestrous cycle resembles with that during the first 14d of early pregnancy. Its content reaches the peak value at lOd after oestrus and then declines. The estradiol peak appears at 3d of oestrous cycle, and the level rises again before oestrus. The concentration of LH declines after oestrus. The level of follicle stimulating hormone reaches the peak value at lOd after oestrus (2.40mIU/ml).

发情周期：民猪发情周期孕酮的含量与妊娠早期14天内的变化极其相似，在发情后第10天达峰值，而后开始下降；雌二醇水平在发情后第3天出现高峰，下次发情前开始升高；促黄体素在发情当天达最高水平，然后开始下降；促卵泡素在发情后的第10天达到峰值(2.40mIU/ml)。

Liver pain, or even death in cirrhotic ascites; Paragonimiasis infected cysts, child or adult worms in human tissues and organs within the transitional, If staying in the lung, patients would cough, chest pain and other discomfort, live in the brain occur in epilepsy, hemiplegia, live mainly in the liver to the liver, liver pain, will live in the subcutaneous form transitional mass or nodule; Fasciolopsiasis worm infection can suffer from abdominal pain, diarrhea, malnutrition, etc.; Angiostrongylus cantonensis larvae in human migration, lesions in the brain, can cause severe headache, nausea, vomiting, fever and neck hard, there will be serious paralysis, lethargy, coma or even death.

感染食源性寄生虫病后可出现不同的症状或体征：华枝睾吸虫病的危害主要是肝受损，轻症者可出现胃肠道不适症状，重者常有肝肿大、肝区疼痛、肝硬变腹水甚至死亡；感染肺吸虫囊蚴后，童虫或成虫在人体组织与器官内移行，若寄居在肺里，患者会出现咳嗽、胸痛等不适感觉，寄居在脑中则出现癫痫、偏瘫等情况，寄居在肝内主要表现为肝大、肝痛，寄居在皮下则形成移行性包块或结节；感染姜片虫后可出现腹痛、腹泻、营养不良等；广州管圆线虫的幼虫在人体移行，病变集中在脑组织，可引起剧烈头痛、恶心、呕吐、发热及颈硬等，严重者会出现瘫痪、嗜睡、昏迷甚至死亡。

The results indicated that the typical apoptosis was induced by PRRSV in lungs and uteri. The ultrastructural changes showed different characterizations at different stages of the infection. During the early stage from 8th h to day 3 postinfection,the apoptosis cells shrank,the cytoplasm concentrated,the chromatin condensed,the kernel disaggregated and the endoplasmic reticulum dilated. During the middle stage from day 5 to day 9 postinfection,the apoptosis cells evidently shrank,the cell membranes protu berated,the plastosome manifolded,and apoptosis bodies were found. During the last stage from day 10 upwards postinfection,the apoptosis bodies degenerated and disappeared,and no inflammation occurred. Few apoptosis cells were necrotic.

结果表明，PRRSV可诱导宿主肺和子宫发生典型的细胞凋亡，表现为细胞的超微形态结构随着病毒感染进程出现不同的特征性变化，早期（感染后第8 h至第3 d）凋亡细胞多表现为细胞体积缩小，细胞质密度增强，染色质浓缩，核仁解体，内质网扩张；中期（感染后第5 d至第9 d）凋亡细胞体积显著缩小，细胞膜突起，线粒体增多，有凋亡小体形成；晚期（感染后第10 d以后）凋亡细胞形态多表现为凋亡小体降解和消失，少数凋亡细胞在凋亡晚期表现为坏死细胞。

After pedicle screw and rod system was placed, a bone block harvested from the posterosuperior iliac with a circular saw with diameter of 1.2 centmeter was implanted into the intervertebral space.

椎弓根钉棒系统固定后，用直径1.2 cm环锯在髂后上棘上端髂嵴处取髂骨植入椎间隙，然后调整钉棒系统，使其椎间隙适当缩小，植入椎体间及嵌入封口的髂骨块挤压牢固后，将钉棒系统固定。

Recent studies of abyssal peridotites show that they are not melt residues of batching or fractional melting of pyrolite directly after melt extraction of MORB. Petrological studies of peridotites from Luobusa ophiolite massif also indicate that the mantle peridotites are not simple residues of primary pyrolite either.

罗布落蛇绿岩橄的镜下观察和岩石化学资料也证明，它不是原始地幔岩经局部熔化后的残余物，而且由来自更深层的地幔过渡带的硅酸盐超高压矿物底辟上升经熔取出玄武质熔体后，再次局部熔化后的残余物。

Methods:Human CD80 cDNA was transfected into B16 melanoma cells by lipofectin-mediated gene transfer before the expansion of tumor cells were tested by MTT method in vitro;C57BL/6 mice were inculated subcutaneously either mock-transfected B16 cells (B16-neo)or CD80-transfected B16 cells (B16-CD80),then the latency,survival times and tumor mass growth were investigated.The lymphocytes were examined for both proliferation indices and specific cytotoxic activity by MTT method and improved LDH-releasing method,after syngenic Mixed Lymphocyte tumor cultures.

通过脂质体介导将CD80基因导入小鼠黑色素瘤B16细胞后，利用SABC法检测CD80的表达；MTT法测定肿瘤细胞体外增殖能力；将肿瘤细胞接种至同源小鼠皮下后观察成瘤期、荷瘤小鼠存活期及肿瘤结节生长速度；在同源淋巴细胞肿瘤细胞混合培养后，通过测定淋巴细胞增殖指数和CTL杀伤活性检测肿瘤细胞的免疫原性。

Midwifery were quickly placed palace at the bottom with one hand, thumb in the anterior, posterior wall of the remaining four means for Palace at the end of even a massage by massage the uterus starts to contract, can be placed in the vagina before the one hand fist fornix, to withstand the uterus anterior abdominal wall push the other hand since the back wall of the uterus, so that the uterus flexion, the relative pressed his hands to massage the uterus.

助产者迅速用一手置于宫底部，拇指在前壁，其余4指在后壁，作均匀有按摩宫底，经按摩后子宫开始收缩，亦可一手握拳置于阴道前穹窿，顶住子宫前壁，另一手自腹壁按压子宫后壁，使子宫体前屈，两手相对紧压子宫并作按摩。

Medical grade SiO was injected into cat eyes after gas-compression vitrectomy. Balanced salt solution was injected into the other eyes as a control. All cats were examined with biomicroscopy and ophthalmoscope pre and postoperatively; intraocular pressure was also measured regularly. After 1, 3 or 6 months of cat's survival, the morphology of RGCs labeled with retrograded HRP injected into the bilateral lateral geniculate nucleus and reacted with diaminobenzidine was quantitatively analyzed.

30只健康成年家猫，气体玻璃体切割后实验眼注入医用硅油，对照眼注入平衡盐溶液，术后定期眼部检查并监测眼压。1、3、6月后进行形态学实验：（1）微电极定位双侧外侧膝状体并注入辣根过氧化物酶，48～60小时后灌注固定，剥取视网膜行二甲基联苯胺成色反应；（2）视网膜尼氏染色；（3）眼球矢状切片常规HE染色。

Significant depletions in serotonin were evident in the hippocampus 1 h and in the striatum 24 h after the first dose and remained reduced 78 h later.

首次剂量后1h，海马五羟色胺明显耗竭，首次剂量后24h，纹状体五羟色胺亦明显耗竭， 72h后仍持续减少。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。