后体

Along with the fluconazole solution density rise,the experimental two kind of strain various glucose density is higher,showe d the glucose consumption are less,takes the logarithmof the medicine de nsity,discovered the logarithm the medicine density and each glucose den sity presents the linear relations;Carries on the analysis comparison to under the fluconazole function two kind of strain linear relations,disc overed the relations of the two strains has the nonuniformity.3 Compare the fluconazole induction reaiatance SC5314 strain and sens itive strain compares,its difference gene expression mainly concentrates in:The code proteinase body and the protein hydroltyic enzyme gene,in the code sugar fat metabolism process is connected the protein gene,the cell cycle correlation gene,the duplication and the translation adjustme nt correlation gene,the stress response correlation gene,the line plast ochondria correlation gene,the cell wall function related gene.4 Candida albicans SC5314 induction resiatance strain was processed b y Xianglian solution,its expression change gene mainly is:Code stress re sponse family protein gene,biomembrane relevant gene,a code proteinase body gene race,code cell cycle related protein gene,duplication and tra nslation adjustment related protein gene.5 The clinical reaiatance strain Candida albicans was processed by Xi anglian solution,its expression change gene mainly is:Codes the hot sho ck protein gene,the serine/threonine protein activating enzyme gene,the proteinase body family gene,the regulation copies and translates the ge ne.

随着氟康唑药液的浓度上升，试验的两种菌株各孔葡萄糖浓度越高，说明葡萄糖消耗越少，经过药物浓度取对数后进行分析，发现取对数后的药物浓度和每孔中葡萄糖浓度者呈现线性关系；对氟康唑作用下的两种菌株的线性关系进行分析比较，发现对两种菌株作用具有不一致性。3氟康唑诱导的耐药SC5314菌株与诱导前的敏感株相比，其差异基因表达主要集中在：编码蛋白酶体及蛋白水解酶的基因，编码糖脂代谢过程中相关蛋白的基因，细胞周期相关基因，转录及翻译调节相关基因，应激反应相关基因，线粒体相关基因，细胞壁功能相关基因。4白念珠菌SC5314诱导耐药株经香莲外洗液作用后，其表达变化的基因主要是：编码应激反应家族蛋白的基因，生物膜相关性基因，编码蛋白酶体基因一族，编码细胞周期相关蛋白基因，转录及翻译调节的相关蛋白基因。5白念珠菌临床耐药菌株经香莲外洗液作用后，其表达变化的基因主要是：编码热休克蛋白基因，丝氨酸/苏氨酸蛋白激酶基因，蛋白酶体家族基因，调控转录及翻译基因。

In 52 days, has dark red vaginal secretions, to another hospital to do a B-, has original heart tube Pollex Mobile, fetal survival, Baotai, doctors opened five 1000ml of HCG, 5 support 20MG progesterone, VE, 5mg folic acid tablets. 5 days later still pink secretions, went to another hospital for a week hospitalization, during the first three days, the color of normal, observed two days, due to shampoo hair, fever 37.5, drank two bags after two hours Banlangen on the anti-fever, and the day of the middle of the night and pink secretions, two days after the normal color, it discharged, the drug is VE, 5mg folic acid tablets, needle 2000ml day of HCG and progesterone 20mg, 20mg progesterone another day.

在52天时，阴道有暗红色分泌物，去医院又做了B超，有原始心管博动，胎儿存活，保胎，医生开了5支1000ML的HCG、5支20MG黄体酮、VE、5mg叶酸片。5天后还有粉红色分泌物，又去另一家医院住院治疗一个星期，期间头三天后，颜色正常，观察了二天，由于洗头吹风，发烧37.5，喝了两包板蓝根两个小时后就退烧了，当天半夜又有粉红色分泌物，二天后颜色正常，就出院了，药是VE、5mg叶酸片、针是一天2000ml的HCG和20mg黄体酮、另一天20mg黄体酮。

With X-ray diffraction and TEM analysis,it is proved that the microstructures of both undeformed and deformed matrixes are austenite and carbide with some striation structure in the matrixes,the amount of stripes in the deformed matrix is obviously more than that in the undeformed matrix.

通过X衍射和电子显微分析证明，变形前和变形后的基体组织都是奥氏体基体加上碳化物，变形前后的基体中均存在条纹状组织，且变形后基体中的条纹数量明显高于变形前基体中的条纹数量。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

LREE depleted spinel lherzolite xenoliths are the 2%～15% partial melting residues of spinel lherzolite with 2 times REE concentration of chondrite. LREE enriched spinel lherzolite xenoliths are the prodiuct that 1% partial melting of garnet lherzolite derived melts mix in ratio of 2% with most LREE depleted spinel lherzolite.

轻稀土亏损的尖晶石二辉橄榄岩包体，是2倍球粒陨石稀土元素丰度的尖晶石二辉橄榄岩地幔经过2％～15％部分熔融后的残留体；而富集型尖晶石二辉橄榄岩包体可能为，来自深部石榴石二辉橄榄岩源区1％熔融形成的熔体以约2％的比例隐交代轻稀土元素最亏损的包体代表的幔源区后形成的。

Methods: Data from the Fracture Prevention Trial and the Multiple Outcomes of Raloxifene Evaluation trial were analyzed to determine the incidences of new adjacent and new nonadjacent vertebral fractures in the placebo groups and the effect of treatment with raloxifene and teriparatide on the incidence of new adjacent vertebral fractures as compared with that of new nonadjacent vertebral fractures.

研究数据来自于骨折防预试验中心，比较雷洛昔芬与安慰剂组对椎体骨折后再发生邻近椎体骨折及非邻近椎体骨折的发生率以及应用特立帕肽、雷洛昔芬对椎体骨折后再发生邻近椎体骨折及非邻近椎体骨折的风险情况。

They are most abundant in quartz of pegmatitic leucosomes and granitic rocks, and represent the fluid appearing in their cooling stage. The DL(H2O) of such fluid is 0.93～0.96g/cm3 corresponding to P≈0.6GPa which is compatible with condition when the rapid decompression in this region was ended. 3 CO2-H2O two or three phases inclusions. They are the most widespread, and more frequently in clusters and in intragranular tails in quartz. The relative content of CO2+CH4 and H2O is considerably variable. They are also characterized with lower density (0.6～0.8g/cm3) and low pressure about 0.3～0.4GPa, and may be originated by mixture of carbonic liquid from breaking of most inclusions of peak stage and aqueous liquid of magmatic source. After entrapment, the further decrease of temperature to lower than 330℃ made such fluid separated to two or three phases.

主要集中于伟晶质脉体和花岗岩中，是这期岩浆冷凝过程析出的流体相当时就被封闭所成，密度为0.93～0.96g/cm3，相应压力约0.6GPa，这与峰期后迅速减压过程结束时的条件相符。3CO2-H2O两相和多相包体，分布最广，成簇状和拖尾状包体群，碳质和H2O相对含量很不均匀，整体密度相对最低，一般为0.6～0.8g/cm3，相应压力为0.3～0.4GPa，它们可能是大幅度减压过程中第一类包体大量爆裂析出的碳质流体与第二类H2O溶液流体在各处以不同比例混溶所成，它们被封闭后在降温至330℃以下时分裂成两相或三相包体。

The program of AER+RES was similar to AER, but went through resistance exercise 20min in base section of every exercise trail.Result: Prior to intervention, adiponectin levels were significantly correlated with BMI、 WHR、 COS、 LN、 LN、 TG、 LG10、 LN、 weight、 waist、 FM、%F, and leptin levels were significantly correlated with BMI、 WHR 、 LN 、 LN 、 TG 、 HDL-C 、 LG10、 apoB 、 apoA1/apoB、 waist、 F1M、%F. Stepwise multiple regression analysis revealed that LG10; COS and waist was an predictor of basal adiponectin levels , and %F,apoB and WHR was an predictor of basal leptin levels. In early obesity, adiponectin and leptin can be worsened , along with the changed of body component, fasting plasm glucose and lipid accumulation. After intervention, both group were significantly reduction in FM,%F,weight, WHR,FPG and TG, while HDL-C had significantly increased.AER exhibited significant in leptin, while AER+RES did not show the change. Insulin and HOMA-IR were changed in both groups. The control group exhibited no significant change in any variables. Although adiponectin levels were unchanged in the three groups, a significant negativecorrelation between delta %F, delta WHR and delta FPG was observed. Futhermore, if compare the degree of delta weight, we fought a significant increased in adiponectin between the group of delta weight higher (=3kg) than delta weight lower(=0kg), delta %F, delta WHR and delta weight were significant negative correlation with delta leptin.

结果：绝经妇女脂联素水平与体重指数、腰臀比、空腹血糖、空腹胰岛素、LEP、TG、极低密度脂蛋白胆固醇、胰岛素抵抗指数、体重、腰围、体脂含量、体脂百分比显著相关；瘦素水平与BMI、WHR、FINS、LEP、TG、HDL-C、VLDL-C、载脂蛋白B、apoA1／apoB、腰围、FM、％F等指标显著相关；多元逐步回归分析显示LG10、COS和腰围是基线脂联素水平的预测变量，而％F，apoB和WHR是基线瘦素水平的预测变量；在肥胖前期，脂联素、瘦素已经出现明显不良变化，同时也伴随着体成分、空腹血糖和血脂的异常；运动实验后，两个运动组的FM、％F、体重、WHR、FPG、TG都显著下降，而HDL-C显著上升；AER组瘦素显著下降，而AER+RES组则没有显著变化；两组的胰岛素和HOMA-IR水平都有显著下降；对照组所有指标都没有明显变化；尽管运动后脂联素水平没有显著变化，但是脂联素变化量与％F、WHR和血糖变化量显著负相关，而且如果按照运动后体重下降的程度比较，则体重下降大(≥3kg)的受试者比下降小的(≤0kg)脂联素水平有明显升高；运动后瘦素水平的变化量与％F，WHR及体重的变化量显著负相关。

Trypsin treatment resulted in a remarkable decrease of the PsbS protein amount in the thylakoids from saturating light-preilluminated but not in those from dark-adapted soybean leaves , compared with that of the control (from dark-adapted soybean leaves and without trypsin-treatment) thylakoids .

与对照（从暗适应大豆叶片提取、没有用蛋白酶处理的类囊体，DU）相比，用胰蛋白酶处理从饱和光照射大豆叶片提取的类囊体后，类囊体的PsbS量显著降低，但是用胰蛋白酶处理从暗适应的大豆叶片提取的类囊体后，类囊体的PsbS量没有显著变化。

Home position shear tests of slope rock, point loading tests and indoor AMD simulation action tests in XingQiao concave slope had show shearing strength and coherence between body layers of quartz sandstone decrease by 13.2%~21.3% after attacked by AMD, the angle of internal friction between layers is decreased by 5.0%~7.5%, in general, AMD affects the rocks mechanical property at range of 60~100m interior the perpendicular slop. Data analysis shows: 1 The actions to slope are major focused on zone of weakness, shearing strength of slope foot and AMD centre are reduced by 52% and 64.7% separately. 2 The explosion stress wave of long distance field has obviously increasing influence to disturbance amplitude value of slope with increasing of the slope height and distance, the response of the top of slope and fault region are obvious as well as damage effect; the foot of slope is with smallest disturbance amplitude value, the disturbance amplitude value of the step close to free face is obviously bigger than the interior of slope. 3 With the coupling effect of the AMD and explosion loading, the stress distribution inside of slope mainly presents characters of explosion stress field; the area affected by AMD becomes a weak area of shearing stress.

新桥深凹露天矿边坡岩体的原位剪切试验、点载荷试验、室内模拟AMD作用试验表明：AMD蚀化后的石英砂岩，岩体层面间的抗剪强度和粘聚力减小的幅值约为13.2%~21.3%；层面间内摩擦角减小幅值在5.0%~7.5%之间；AMD一般影响垂直边坡体内部在60~100m范围内的岩体力学性质数值分析表明：AMD对边坡体的作用主要集中在弱化区域内，边坡体的底脚和AMD作用中心区域的剪切应力减少了52.0%和64.7%；(2)随着边坡体高度和距离的增加，远场爆炸应力波对边坡扰动幅值的增加趋势明显，边坡顶部和断层部位的响应突出，损伤效应明显；边坡底脚扰动幅值最小，临空面附近台阶的扰动幅值明显大于边坡体内部；(3)AMD与爆破荷载耦合作用后，边坡体内部的应力分布主要呈现出爆炸应力场的特征，AMD影响区域形成了一个剪切应力弱区。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐