后体

On the other hand, after 7-week gavaging abidingly, the effect of II-RMeOH on the bleomycin-induced chronic pulmonary fibrosis was investigated. The study was evaluated by measuring the hydroxylproline, collagen, transforming growth factor-β1 (TGF-β1), and microwave Masson's trichrome stain.

经由肺脏检体的组织切片染色后，可以用半定量型态学分析来判断肺脏的发炎情形；经过判读后，结果和测定出来的生化值有相同的趋势，在投予板蓝根甲醇粗抽物后，减轻了肺脏的发炎程度。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

The shape of chloroplast didn't change after seedling was treated by GA〓, but the number of lamllasomes increased, and starch grain enhanced. These changes were accord to that of the rate of photosynthesis. Mitochondria varied greatly, the structure of mitochondria could see vaguely and carinace almost deliquesce after 4days. Enrolucrum emerged until the seventh day after treatment and endomembrance of mitochondria recovered gradually, cristae could be seen. On the other hand, the endomembrance of mitochondria in CK (normal non-bolting) was obvious and the quantity of cristae in the mitochondria was much more than the GA〓 treatment. This showed that respiration might decrease after GA〓 treatment while reduced the depletion of photosynthesis products and enhanced bolting.

GA〓处理后抽苔过程中叶绿体形状未发生变化，但类囊体增多，淀粉粒增多，这种变化与光合速率的变化吻合；线粒体变化较大，处理后4天线粒体结构较模糊，嵴不清晰，7天时外膜形成，内膜也逐渐恢复，能见到嵴，而对照线粒体内膜结构明显，嵴清晰数量也多，说明GA〓处理后，呼吸作用降低，减少光和产物的消耗；细胞膜无破损，较光滑完整，说明对细胞膜没有伤害。

Result: 96 cases acute adhesive intestinal obstrnction patients recovered after conservative treatment. 4 cases that had peritonitis were treated with enterotomy.

结果：112例保守治疗后96例痊愈出院，其中16例2月后再次发作。9例有腹膜炎体征入院后行急诊手术治疗，术中出现有肠坏死作肠切除者4例。

Cacses acute adhesive intestinal obstrnction patients who had peritonitis were operated.Result: 96 cases acute adhesive intestinal obstrnction patients recovered after conservative treatment.4 cases that had peritonitis were treated with enterotomy.

结果：112例保守治疗后96例痊愈出院，其中16例2月后再次发作。9例有腹膜炎体征入院后行急诊手术治疗，术中出现有肠坏死作肠切除者4例。

Results一. Culturing and identification of human conjunctiva cells1. morphous of conjunctiva cellsCells were harvested by tissue cultivation after digestion. Ponderosus cells adhered after 48 hours. Cells were shown in shape of round, ellipse and polygon. Cell body was loose and lucency with nucleus in center, cell membrane was also clearly seen. Cells were arranged in inlay and fuse in film after 12-14 days.2. Immunochemistry testNomogeneous immunochernical positive granules against CK13 were observed in endochylema of human conjunctiva cells.3. growth curve of conjunctival cellsSubcultured cells were aged and feeble after 5th passage.

结果一、正常人结膜细胞的培养与鉴定1、活体细胞的形态消化后组织块培养法：48小时后见大量上皮细胞贴壁，细胞不规则呈圆形、椭圆形、多边形，细胞体肥大透亮，细胞核位于中央，胞膜清楚，约12-14天后细胞呈镶嵌状排列，融合成膜状。2、免疫组化鉴定培养的人结膜上皮细胞CK13免疫组化染色见阳性颗粒均匀分布于胞浆中。3、培养结膜细胞生长曲线传代培养多数样本于P5代以后开始出现衰老征象。

The tumefaction extents of back metatarsus of rats before and after inflammation, times of every mouse twist in 30 minutes after celiac injection (0.7% glacial acetic acid 10 mL·kg-1), times of abnormal excretion in 1-6 h after pouring the senna, respectively, were observed.

分别观察致炎前和致炎后大鼠足跖肿胀程度、腹腔注射0.7%冰醋酸10 mL·kg-1后30 min内每只小鼠扭体反应发生次数及灌服潘泻叶后1～6 h内小鼠排异常便次数。

The static shape of tongue after reconstruction with submentalental island flap were acceptable. the dynamic function such as movement, speech and swallowing, were all well reformed. cheek mucosity were velvet and has no plump or overstaffed . 4 patients were all given radiotherapy after operation.the figuration and function of tissue defect region,that reconstructed, had no obviously change after radiotherapy.

修复后的舌体静态形态佳；动态表现舌的运动不受限，语音清晰，吞咽功能良好；修复后的颊粘膜表面光滑，无肥厚臃肿，不影响上下颌的咬合。4例术后均给予放疗，放疗后舌、颊部无异常变化。

After 7 days of parabiosis, the mice were separated and the skins from one to the other were transplanted.

吕艳将BALB／c(H-2d)小鼠及C57BL/6(H-2b，B6)小鼠的脾细胞分别经尾静脉注射给对方，2d后再分别腹腔注射环磷酰胺150mg／kg，CY注射后1d对BALB／c及B6小鼠进行联体，1周后分开并进行相互间的植皮。

Measure the items on before and after the two courses of treatment respectively as follows:① Syndromes Investigation: to adopt the scoring methods of symptoms and signs with the certain standard scoring table of symptoms and classifying table of signs for the patients, on the day before and after the two courses of treatment respectively ,② Signs Investigation: the mucoma visibility, swelling or hyperemia conditions, or gloyp and polypoid change in the inferior and middle nasal conchas, nasal septum, etc with a nasal endoscope on the day before and after the two courses of treatment respectively,③ Test of the lung functiaon on the previous day before and after the two courses of treatment respectively.

治疗组在治疗前、两个疗程结束后分别进行以下项目的观察：①临床症状的评定：治疗前及两个疗程结束后当天对患者鼻塞、鼻痒、流涕、喷嚏症状的程度进行评分；②体征的评定：治疗前及两个疗程结束后当天采用鼻额镜肉眼观察患者下鼻甲鼻粘膜颜色、肿胀情况，及中鼻甲是否可见，有否息肉样变等；③治疗前及两个疗程结束后即刻予以检测肺功能。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。