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In order to breed new germplasm for Narcissus tazetta,the bulb of Narcissus tazetta is used as explants.The adventitious buds is induced in MS media with hormone combination.γ-ray irradiation treatment of Narcissus tazetta combined with tissue culture is studied.Three materials,including the bulb basal plate tissues inoculated for 7 days,tissues transferred in fresh differentiation culture medium for three days after inoculated for 25 days,and small bulbs in vitro,are studied.

以带鳞片叶的鳞茎盘为外植体,接种在添加激素的MS培养基上,通过激素组合筛选合适的培养基获得不定芽,同时对在预分化培养基上接种7D的外植体、接种25D后转接到分化培养基上3D的外植体、诱导产生的试管小鳞茎进行辐照诱变处理,研究了60COΓ射线对中国水仙丛生芽分化以及小鳞茎生长的影响。

In cider to breed new gern1asm for Narcissus tazetta, the bulb of Narcissus tazetta is used as explants. The adventitious buds is induced in MS media with hormone combination.γ-ray irradiation treatment of Narcissus tazetta combined with tissue culture is studied. Three materials, including the bulb basal plate tissues inoculated for 7 days, tissues transferred in fresh differentiation culture medium for three days after inoculated for 25 days, and small bulbs in tarn, are studied.

以带鳞片叶的鳞茎盘为外植体,接种在添加激素的MS培养基上,通过激素组合筛选合适的培养基获得不定芽,同时对在预分化培养基上接种7d的外植体、接种25d后转接到分化培养基上3d的外植体、诱导产生的试管小鳞茎进行辐照诱变处理,研究了60Coγ射线对中国水仙丛生芽分化以及小鳞茎生长的影响。

When embossing district zhaiyanzhen rubberand drum RADIUS plus the paper thickness is greater than the radius of embossing drum cylinder, paper margin rubber drum circle distance greater than the RADIUS for the imprint of the rollers, paper for the cylinder body relative to the surface of the cylinder of nano-imprint lithography drum slide forward, imprinted deformation and dot gain no significant adverse effects.

当不抬印区某处的橡皮滚筒半径加上纸薄不小于不抬印滚筒筒体半径时,纸张背背距橡皮滚筒圆心的间隔不小于不抬印滚筒筒体半径,纸张背背不绝不差于不抬印滚筒筒体暗地上后滑不静,这不差痕迹变形及网不背增不小无澄辉不良感化。

The microstructure of hardened layer and formation mechanism were investigated.The microstructure of hardened layer consist of the completely hardened region and the transition region.The completely hardening region is composed of fine acicular martensites,residual austenites and a few dot-like carbonides.From surface to bottom in the hardened layer,the microstructure varies from fine to coar...

结果表明:采用适当的磨削参数可满足该钢所要求的淬火温度,进而获得适宜的淬硬层显微硬度和强化层深度;磨削淬火层由完全硬化区和过渡区组成;完全硬化区主要由细小针状马氏体组成,从表面到里层,组织形貌呈现&细→粗→细&的变化规律,其形成机理是热-力耦合作用影响磨削淬火过程中的奥氏体晶粒大小及其位错密度,并将直接影响转变后的马氏体组织形貌。

Expression patterns of genes differentially expressed in starch metabolism and cell cycle during endosperm development7 DAP kernals, 15 DAP endosperms and 25 DAP endosperms of B73, ae/wx and sh1 were used to study the expression patterns of 18 differentially expressed genes in sugar metabolism and 9 differentially expressed genes in cell cycle. We found that the expression patterns of Pul, SBEⅡa, UGPase, SUS3, SSⅡa in sh1 and Sh2-1, SUS3, SSⅡa in ae/wx were altered, revealing that these genes may interact with each other.

碳水化合物代谢基因和细胞周期基因随胚乳发育的变化以B73、ae/wx、sh1授粉后7天种子、15天和25天胚乳为材料,分析18个糖代谢差异基因和9个细胞周期调节相关的差异基因的表达模式,研究发现sh1突变体中Pul、SBEⅡa、UGPase、SUS3和SSⅡa基因表达模式发生改变,ae/wx突变体中Sh2-1、SUS3和SSⅡa基因表达模式发生改变,揭示了突变体对这些基因的巨大影响,反映了它们可能存在相互作用。

The soft temperature of copper matrix could be effectively improved and thermal expansion of copper matrix could be effectively restrained by the AlN particles; Under the action of porosity and thermal stresses, the thermal expansion curve of AlNp/Cu composites became non-linear with the particle volume contents increasing to a certain extent.

结果表明:AlN的加入能够提高铜基体的软化温度且对铜基体的热膨胀起到明显的约束作用;在孔隙与热应力共同作用下,AlN颗粒含量达到一定程度时,AlNp/Cu复合材料膨胀曲线随温度的上升将产生非线性变化;加热过程中热应力造成的基体塑性变形使热循环后复合材料存在残余正应变。

Flounder FoxD1 mRNA was over expressed in one cell of the two-cell stage zebrafish, MyoD expression in the somites on one side was reduced, but the expression in the adaxial cells was not affected; Myf5 expression in the presomitic mesoderm, adaxial cells and somites on one side was reduced.

在斑马鱼中过量表达FoxD1后,MyoD在一侧体节中的表达受到了严重抑制,而在近轴细胞中的表达未受影响,Myf5在体节前中胚层,近轴细胞,及体节中的表达都受到了抑制。

When aborted rats in group E were treated with the dose of 50 IU/g IFN-γ, expression of TNF-αin this group was compared with the other four groups, the results were as follows: Compared with group A, the nucleus pre-opticus medialis, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami and pars intermedia had a highly expression of TNF-α, in the other nuclei of hypothalamus, neurohypophsis and adenohypophysis, the change was not significantly, the expression of TNF-αin follicle and corpus luteum were decreased distinctly, the variation in uterus was slight and no difference was exsited; Compared with group B, the expression of TNF-αin nucleus nucleus lateralis hypothalami, nucleus ventromedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis and uterus presented a remarkable enhancement, conversely, the TNF-αexpression in the nucleus pre-opticus medialis, nucleus pre-opticus suprachiasmaticus, nucleus supra-opticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, neurohypophsis, follicle and corpus luteum had been significantly decreased; Compared with group C, the nucleus pre-opticus medialis, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis, pars intermedia and uterus had upregulated the expression of TNF-α, however, it was decreased in the nucleus periventricularis hypothalami and corpus luteum; When compared with group D, the expression of TNF-αin nucleus lateralis hypothalami, nucleus dorsomedialis hypothalami, nucleus ventromedialis hypothalami, pituitary, follicle and ovary stroma were obviously enhanced, while in the nucleus supra-opticus and nucleus arcuatus hypothalami, the situation was definitely opposite, in the other parts, the change was not significant.

外源腹腔注射50 IU/g IFN-γ后,同A组相比:E组TNF-α免疫阳性产物在下丘脑视前内侧核、视前大细胞核、室周核、腹内侧核、背内侧核、弓状核、垂体中间部显著增高,在下丘脑其它核团、神经垂体、垂体前叶较A组差异不显著,在卵泡、黄体中阳性产物表达均较A组显著降低,在子宫中表达较A组变化不明显;同B组相比:E组TNF-α免疫阳性产物在下丘脑外侧核、腹内侧核、弓状核、垂体前叶、子宫均显著增高,在视前内侧核、视交叉上核、视上核、视前大细胞核、室周核、室旁核、神经垂体、卵泡、黄体中阳性物质表达均显著降低;同C组相比:E组下丘脑视前内侧核、腹内侧核、背内侧核、弓状核、垂体前叶与中间部、子宫均显著增高,在室周核、黄体中均显著降低;同D组相比:E组下丘脑外侧核、背内侧核、腹内侧核、垂体各部、卵泡、卵巢基质均显著增高,在视上核、弓状核显著降低,在其它部位表达变化差异不显著。

Results: Embryoid bodies grew well in Collagen Gel, the differentiated cells grew into the gel gradually; electronmicroscope showed that some induced cells were rich in Golgi's bodies and endoplasmic reticulums, a few secretory granules were also visible, which was similar to human adult thyrocyte.

结果 拟胚体在鼠尾胶原上生长状态良好,分化细胞贴壁后逐渐陷入鼠尾胶原;电镜下诱导细胞高尔基体、内质网丰富,部分诱导细胞胞质内见不典型分泌颗粒,与人成体甲状腺细胞超微结构类似。

According to the failure cause summary of abandoned elements, it is determined the twisting and winding process of carbon fiber ply yarn and high temperature holding process of carbon composite were key steps during the preparation of carbon heating element.

在此基础上研制了碳素纤维电热体股线的捻制缠绕设备并建立其工艺计算模型,开发了碳素复合材料电热体的高温定型装备并优化了定型后电热体的脱芯工艺。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。