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The ultraviolet spectrophotometry method as determining the content ofsapogenins was used to evaluate the change of the quality in Dioscorea Zingiberensis C.H. Wright before or after supercritical CO_2 fluid extraction. The content of sapogeninswas 2.03% and 1.84% respectively in original and depurative sample, which showed thatdepuration of Cu, Cd, Pb in Dioscorea Zingiberensis C. H. Wright by supercritical CO_2fluid extraction had little influence on the active ingredient.

2采用超临界CO_2流体萃取法提取黄姜中甾体皂素,以可见分光光度法测定其含量,得到黄姜原样品及重金属净化后样品中甾体皂素含量分别为2.03%和1.84%,超临界CO_2流体络合萃取黄姜中Cu、Cd、Pb后对其有效成分影响不大。

The best optimum conditions of the Agrobacterium mediated genetic transfornation were that leaves explants were immersed in the diluter 10 times Agrobacterium bacilli at OD600 of 0.6 for 8 minutes after being pre-cultured for 2 days,and then co-cultivated for 3 days.Then these explants were transferred to selected and rooting medium which contained 50 mg/L Kanamycin and 500 mg/L Carbenicillin.According to the transformation system, 54 transgenic plants were initially screened,then after PCR analysis and RT-PCR analysis 25 transgenic plants were obtained.

根癌农杆菌介导的芪合酶基因遗传转化的最佳条件为:将预培养2 d的烟草外植体,用稀释10倍的GV3101菌株菌液(OD600=0.6)浸染8 min后,共培养3 d,然后转入含卡那霉素50 mg/L和羧苄青霉素500 mg/L的筛选分化培养基上诱导分化,待抗性芽长到2~3 cm后,转入含Km 50 mg/L和Cb 500 mg/L的筛选生根培养基上进行筛选,在以上最佳遗传转化条件下,初步筛选到54株转化体,经PCR和RT-PCR检测获得25株转化烟草植株。

For the more, we study the anchor and hanging performances of expansion bolt in the masonry. The results indicate that this kind of masonry can satisfy the requirements of anchor or hanging force in decoration of building.

通过锚栓在砌体中的后锚固性能试验研究和墙体吊挂力试验研究,表明砌体具有一定的锚固抗拔性能和抗弯剪荷载能力,基本能够满足后装修较小锚固力及吊挂力要求。

The results showed that diploid and tetraploid had higher root activity, flagleaf soluble protein content, SOD and CAT activity before anthesis. However, the rootactivity, flag leaf soluble protein content, SOD and CAT activity of diploid and tetraploiddeclined more quickly and became lower than those of hexaploid species from 15 days afteranthesis. While MDA content increased more quickly and became higher than those ofhexaploid species from 5 days after anthesis. Correlation analysis showed that delaying the flag leaf senescence can promoted transfer of nitrogen and dry matter, increasedaccumulation of nitrogen and dry matter after anthesis, enhanced grain and protein yield.

结果表明,与普通小麦和小黑麦相比,二倍体和四倍体材料开花以前具有比较高的根系活力、可溶性蛋白含量、SOD和CAT活性;开花以后,根系活力、旗叶可溶性蛋白含量、SOD和CAT活性快速下降,MDA含量快速上升,衰老加快;相关分析表明,延缓小麦旗叶衰老进程,可以促进花后氮素和干物质运转,增加花后氮素和干物质积累,提高籽粒产量和籽粒氮素积累量。

All had discomfort sensation on the pharyngeal and glossal portion, and movement of the tongue was constrained for 2-4 days and became normal after 5-7days.

所有患者在开始进食时有咽部、舌部不适,手术2~4d后舌体运动略有受限,一般在5~7d后舌体运动感觉正常。

As to the doctrine called the Real Presence,[22] the council condemns: those who, denying that Jesus Christ, God and Man, is truly, really, substantially present in the sacrament of the Holy Eucharist, hold instead that He is only present as in a sign or image or manifestation of power; those who say that the substance of the bread and the wine remains along with the body and blood of Christ, denying that marvellous and unique changing of the whole substance of the bread into the Body and the whole substance of the wine into Blood, while the appearance of bread and wine still remain--the change which the Catholic Church most suitably calls Transubstantiation; those who say that the Body and Blood of Christ is not there following upon the consecration, but only while the sacrament is in use, while it is being received, that is to say, but not before this or after this, and that in what is left over of the consecrated hosts or particles after communion has been administered, the true Body of the Lord does not remain; who say that the main fruit, or the sole fruit, of this sacrament is the forgiveness of sins; or that Christ the only begotten son of God is not to be adored in this sacrament with the externals of the reverence called latria,[23] and that those who do so adore Him in this sacrament are idolaters; that Christ is shown forth in this sacrament to be received in a spiritual manner, and not also sacramentally and really; that only faith is sufficient preparation for receiving this most holy sacrament.

以中庸所谓真正的存在, [ 22 ]安理会谴责:这些人,是无可否认的耶稣基督,人与上帝,是真的,真的,大幅度目前在圣圣体圣事,举行而是说,他是目前唯一的作为一个标志或形象或表现形式的权力;那些说这种物质的面包和葡萄酒仍是随着身体和血液基督的,是无可否认的壮美和独特的变化,整个物质的面包进入人体和整个物质的酒的血液,而外观的面包和葡萄酒仍是-改变,其中天主教教会最适当呼吁陷于变体说,这些人说,该机构和血液基督,是不是有以下后, consecration ,但只有而圣餐是在使用,而它正在被接受的,也就是说,而不是在此之前或在此之后,以及在什么是遗留下来的该consecrated主机或微粒后,共融一直经管的,真正的机构主不留;人士说,这样做主要水果,或唯一的水果,这圣餐是罪的赦免;或基督独生子的上帝是不被喜欢在这圣餐与外部的崇敬所谓latria , [ 23 ] ,并认为那些这样做,崇拜他,在这圣餐是盲目崇拜者;基督表明,在此圣餐将收到的在一种精神的态度,是不是还sacramentally真的,那只有信仰是有足够的准备接受这个最神圣的圣餐。

The main results were summarized as follows: 1 With the increase of cooling rate, full recrystallization during cooling became difficult to take place and the decrease in dislocation density became slow so that dislocation density in austenite remains higher before phase transformation occurred. 2 Compared with undeformed samples, the CCT diagram under deform conditions has a similar shape, but its position shifts leftwards.

计算结果表明:1随着冷却速率增大,低碳钢热变形后的冷却过程中再结晶变得越来越困难,位错密度的下降变慢,导致在相变前奥氏体中保留着较大的变形存储能;2奥氏体在经历热变形后的CCT曲线与未变形条件相比,明显向左上方偏移。

in order to probe into whether glycine supplementation plays an adjusting role in sports performance and immune functions, the authors divided 30 mice randomly into an exhaustive exercising group (e1), glycine supplemented exhaustive exercising group (e2) and a normal resting control group, with 10 mice in each group, supplemented glycine to mice in group e2 and physiological saline to mice in the other two groups, let mice in groups e1 and e2 do an exhaustive swimming exercise 4 weeks later, applied the trace ch50 hemolysis method to analyze supplement activity, applied the mtt method to perform a quantitative analysis on the lymphopoiesis ability of spleen, and thus revealed the following findings: compared with mice in group e1, the time taken by mice in group e2 to swim to an exhausted state is significantly longer (p.05); total supplement activity in blood serum of mice in group e1 is significantly lower than the same of mice in group s (p<0.01), and total supplement activity in blood serum of mice in group e2 is significantly higher than the same of mice in group e1 (p.01), but still significantly lower than the same of mice in group s (p.05); as observed from the test of the lymphopoiesis ability of spleen, the lymphopoiesis ability of spleen of mice in group e1 is significantly lower than the same of mice in group s, and the lymphopoiesis ability of spleen of mice in group e2 is significantly higher than the same of mice in group e1, but still significantly lower than the same of mice in group s.

四型肽精氨酸脱氨酶在类风湿性关节炎。。。以马来酸酐与氨水为原料热缩聚法合成。。。摘要:为探讨甘氨酸补充对运动能力及免疫功能是否具有调节作用,将30只小鼠随机分成力竭游泳运动组(e1)、甘氨酸补充后力竭游泳组(e2)和静息正常对照组,每组10只。在e2组补充甘氨酸,其余两组给予生理盐水,处理4周后,e1组和e2组均做力竭性游泳运动。采用微量ch50溶血法分析补体活性,采用mtt法定量分析脾脏淋巴细胞增殖能力。结果显示,与e1组比较,e2组游泳至力竭的时间显著延长(p<0.01)。e1组血清总补体活性显著低于s组(p<0.01),e2组显著高于e1组(p.01),但仍显著低于s组(p.05)。在脾脏淋巴细胞增殖试验中观察到,e1组显著低于s组,e2组显著高于e1组但仍显著低于s组。

RESULTS: Fifty minutes after addition of inducing medium, under the optical microscope, BMSCs exhibited a typical morphology of perikaryon. Three hours later, most of cells became into bipolar or multipolar neuron-like cell appearance with cell bodies and processes.

结果:加入诱导液50 min后,光镜下骨髓间充质干细胞呈典型的核周体形态,3 h后大多数细胞转变为双极或多极神经元细胞样形态,出现胞体和突起。

Because it is a hard work to refold the denatured inclusion proteins correctly, especially for those which contain several disulfide bonds. the results?repeatability of some activities is not very perfect.

纯化包涵体后,将产物变性成可溶形式,然后用Ni-NTA对表达产物纯化,SDS-PAGE检测为一条带,包涵体的复性是一项非常困难的工作,尤其是对含有多对二硫键的蛋白质,因此复性后冻干,测它的某些活性时,结果的重复性不太理想。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。