后体

Conclusion］1? It showes a higher stress dodge in the calcar femorale after using various kinds of prosthesis implantation? After using CFR/PSF of lower elastic modulus as handle, the stress dodge rate is lower in femur, but the interface stress is higher, and this is the main factor and reason for the prosthesis loosening? 2? The stress force rule meets the design principle that the stress force decreases gradually in the bone interface from proximal to distal? The stress value in different combination of same prosthesis handle has no significant difference, that showes mechanical factor is not the main standard for selecting prosthesis combination?

［结论］ 1、各种假体植入后均在股骨距处形成较高的应力遮挡，而用弹性模量较低的CFR/PSF作为柄，其股骨相应区域的应力遮挡率较低，但股骨相应界面应力较大，而界面应力过大是产生假体微动主要因素。2、股骨界面从近端至远端呈逐渐增高趋势的应力规律符合该假体的设计原理；相同假体柄的不同组合其股骨和髋臼相应界面应力值无明显差别，实验表明力学因素并不是选择假体组合的主要标准。

The results show that:(1) after MA for 20h, single phase bcc nanocrystalline supersaturate solid solution formed in Fe84Nb7B9 and Fe80Ti8B12 alloys; the thermal stability of nanocrystalline microstructure in Fe80Ti8B12 alloy was improved significantly than that in Fe84Nb7B9 alloy, and the decomposition temperature of supersaturate solid solution decreased in Fe80Ti8B12 alloy.(2) under 30MPa/900℃/0.5h hot-press sintering conditions, the relative density of Fe84Nb7B9 and Fe80Ti8B12 bulk alloys were 96.7% and 95.5%, respectively. Compared with Fe84Nb7B9 alloy (muti-phase microstructure), Fe80Ti8B12 alloy was composed of superfine crystalline (50~200nm) of single phase α-Fe;(3) the magnetic properties of Fe80Ti8B12 bulk alloy σ(subscript s=198.6emu/g, H=54.6Oe were more excellent than that of Fe84Nb7B9 bulk alloy σ(subscript s=162.9emu/g, H=105.1G, it was related to the different phases formed during hot-press sintering and the grain size of nanocrystalline powder.

结果表明：(1)20hMA后，Fe84Nb7B9和Fe80Ti8B12均形成了单相bcc纳米晶过饱和固溶体，相对前者，后者纳米晶组织热稳定性明显提高，过饱和固溶体相分解温度略有降低；(2)在30MPa/900℃／0.5h热压条件下，Fe84Nb7B9和Fe80Ti8B12块体合金相对密度分别为96.7%和95.5%，相对前者，后者由超细晶(50~200nm)单相α-Fe组成；(3)Fe80Ti8B12块体合金软磁性能σ(下标 s=198.6emu/g，H＝54.6Oe明显高於Fe84Nb7B9块体合金σ(下标 s=162.9emu/g，H＝105.1Oe，此与纳米晶粉末在热压烧结过程中所形成相的种类和晶粒尺寸大小有关。

RESULTS:①There was higher tensile stress at the ingual side of symphysis, inside of molar and outside of condyle under the impact of chin.②There was higher tensile stress at the lingual side of symphysis, inside of contralateral molar, outside of contralateral angle, outside of homolateral angle and inside of homolateral body under the impact of body.③There was higher tensile stress at the inside of angle, inside of homolateral condyle, lingual side of symphysis, outside of contralateral condyle and inside of homolateral molar under the impact of angle.

结果：①撞击颏部，其舌侧区，磨牙后区内侧面、髁状突颈外表面以及下颌角外表面具有较高的张应力；②撞击下颌体部，颏部舌侧区，对侧磨牙后区内侧面、对侧下颌角外表面、撞击侧下颌角外侧面、撞击侧体部内侧面具有较高的张应力；③撞击下颌角，其内侧面、撞击侧髁状突颈内侧面、颏部舌侧面、对侧髁状突颈外表面以及撞击侧磨牙后区内侧面具有较高的张应力。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

The results showed that 7-day-precultured embryogenic calli had the best responses for bombardment among 2-7 day' preculture duration. Enhanced transformation efficiency was found in the explants treated with high osmotic medium 4h prior to till 24 h after bombardment. Particles of gold and tungsten were both found suitable for rice transformation and there was no adverse effect when tungsten particles were reduced to 1/3 standard amount for DNA precipitation on transient GUS gene expression. However, it was found that gold particle had higher GUS expression at 120 h post-bombardment. A preliminary microprojectile transformation system with an average of 15-20 blue spots/explant GUS expression was routinely obtained for 'Tainung 67' by using the particle gun with 1,100 psi rupture disk, 6 or 9 cm microcarrier flight distance and 4 mm of macrocarrier flight distance setting.

初步结果显示，以预培养2~7日之胚性癒合组织逐日测试转殖率，以预培养7日之培植体表现最佳；培植体於转殖前4小时至转殖后24小时以高渗透压培养基处理，有助於转殖效率之提高；比较金、钨粒子对转殖率在转殖后48h並无不同，但转殖后120 h取样，则金粒子组有较高之蓝点数；钨粒子用量可减少至1/3標准用量对转殖率並无影响；利用基因鎗在使用1， 100 psi裂碟片、大载片至挡片之距离为4 mm、微载髁至射击目標之飞行距离6或9 cm之设定，GUS基因表现之平均蓝点数均可达15~20个／培植体。

Results: There was no difference of age, BMI, AHI and pulse oxygen between the stricture group and the nonstricture group. The coronal and the arrow diameters of the stricture group were less than those of the nonstricture group. The thickness of the retropharyngeal and lateral pharyngeal wall, and the width and length of the tongue of the stricture group were larger than those of the nonstricture group.

结果：两组患者的一般临床资料无明显差别，舌后区狭窄组的舌后区呼吸道左右径、前后径明显小于非狭窄组，狭窄组的咽后壁、咽侧壁软组织厚度、舌体宽度、舌体长度和舌体截面积等CT测量数据明显大于非狭窄组。

Results: There was no difference of age, BMI, AHI and pulse oxygen between the stricture group and the non-stricture group. The coronal and the arrow diameters of the stricture group were less than those of the non-stricture group. The thickness of the retropharyngeal and lateral pharyngeal wall, and the width and length of the tongue of the stricture group were larger than those of the non-stricture group.

结果：两组患者的一般临床资料无明显差别，舌后区狭窄组的舌后区呼吸道左右径、前后径明显小于非狭窄组，狭窄组的咽后壁、咽侧壁软组织厚度、舌体宽度、舌体长度和舌体截面积等CT测量数据明显大于非狭窄组。

On the PC of the Windows Operation system, the system of 3D reconstruction has been designed by Visual C++ programming language. It includes 3D data import, display of the original and pre-process CT images, acquirement of the data showed vividly on the screen by the improved algorithms about MC and Ray Casting and mutual observation through magnification or demagnification and circumvolving.

论文以此为基础，在PC 上，基于Windows 操作系统，利用Visual C++开发工具，运用计算机图形学和图像处理技术，实现了三维体数据的输入；二维CT 图像数据的显示和预处理；通过阈值对CT 数据进行了分割；运用改进后的算法分别对分割后的数据进行了面绘制和体绘制；对绘制后的数据在屏幕上形象逼真地进行了显示；对显示的数据进行交互式的观察，如旋转、放大、缩小。

Under the incubation conditions such as salinity of 32, temperature of 16℃ and light intensity of 6 000 lx (photonperiod of 12 h︰12 h LD), the first spore division occurred 22 hours after attachment, and further cell divisions formed a multicellular disk after 6 days. The disk which developed most quickly began to germinate burgeons after 80 days.

在水温16℃，海水盐度32，光照强度6 000 lx（光照周期为12 h︰12 h）条件下，鹿角海萝孢子附着后约22 h进行第一次细胞分裂，经过多次分裂，6 d后形成初期盘状体，发育最快的盘状体80 d后长出芽体。

In addition, many studies of patients with AVN showed plenty of special characteristics,such as age distribution and bone quality.Objective:To study the geometric features of proximal femur for the Chinese AVN population,in order to prove the existence of special characteristic of proximal femur for the disease and then develop an AVN-specialized stem evaluated by a validated finite element analysis.Methods:1.The author investigated the standard X-ray data of 127 cases(179 hips) with definite diagnosis of AVN to provide anatomical references for this disease.At the same time,we conducted a meta-analysis of anatomical studies of normal proximal femur data,including the section of LT+20,LT,LT-20 and CFI to identify the potential difference between AVN and normal population.

方法1、通过研究AVN患者标准X线正、侧位片127例(179髋)，获得股骨近端的解剖学数据，利用荟萃分析的方法比较AVN患者股骨近段髓腔特征平面(LT+20，LT，LT-20)及CFI的改变与以往研究数据的差异，并利用创伤性FNF患者资料代替正常人股骨数据进一步比较髓腔前、后、内、外四条曲线段上的差异。2、通过结合现代CAD技术，将内、外、前、后曲线拟合到AVN柄的外形上，选用短柄假体设计，近段设计利用AVN患者骨髓腔CT相对应位置的断面进行修正，设计AVN人工股骨柄。3、计算机三维有限元分析：①使用CAD软件SolidWorks和有限元软件Ansys所建立的髋关节股骨侧假体置换后的三维有限元模型。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。