后中的

But the reflection factor is in a standard filter important coefficient, but uses the alternative means to be very difficult to guarantee front and the retroreflection coefficient value is smaller than 1, and is unable to obtain its correlation function precisely, especially has this kind of serious problem in linear prediction coding equisignal processing, the BURG algorithm can in then be able to determine from the very beginning its exponent number and the transmittance have then overcome a limit question.

而反射系数是格型滤波器中的一个重要系数，而采用其他方法很难保证前向与后向反射系数的值小于1，并且无法精确得到其相关函数，尤其在线性猜测编码等信号处理中存在着这种严重问题，BURG算法能在一开始便能确定其阶数和传递系数便克服了着一限制问题。

The present report deals with the results of some studies on the longevity of the soft-rot organism, Erwinia aroideae Towns.

当軟腐細菌进入土壤中后24小时，軟腐細菌的数量銳减而土壤中的其他微生物数量上昇，以后亦逐漸递減。

In order to cutting down the cost, flocculation of recombined Erwinia carotovora-Eccl3B cell from broth, influence of temperature, pH and oxidants on the separation of PHB were studied; and the method which measure the molecular weight of PHB by intrinsic viscosity was also studied; furthermore, a phenomenon was found that insolubility of PHB in chloroform can be changed by pretreated at over 120℃ for the fist time, and the mechanism of this phenomenon was explored.

为了降低成本，提高生产效率，本文针对发酵法生产PHB过程中存在的，发酵液难以分离沉淀，非有机溶剂提取PHB时分子量严重降低等问题，进行了发酵液的絮凝，及温度、pH、氧化剂对提取PHB影响的研究。并对粘度法测定PHB分子量的方法进行了研究，同时，对于研究过程中发现的，加热后PHB在三氯甲烷中的溶解性变化及其原因进行了探索。

The result of our experiments showed that PAM has little effect on sandy soil. We analyzed that it is because there are little fine glutinosity soil grain in sandy soil.

研究结果初步表明，PAM在砂性土壤中的施用效果不佳，据分析，这是因为砂性土壤中细小粘粒含量较少，施加PAM后，不能形成较多的水稳性土壤团聚体。

The equipment is used in hoe-pressing of sandwiched glass with gumming material under designated temperature and pressure. Inseparable adhesion between glass and gumming material will then be achieved after cooling and the sandwiched glass is made.

本设备用于对胶片法生产夹层玻璃进行热压处理，预合胶片夹层玻璃置于该设备中，在一定的温度压力下，使夹层中的胶片溶化，经冷却后使得胶片和玻璃紧密粘合成夹层玻璃产品。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

A locomotive has passed, never to returnto the scene of our reading; the man strolling by the river hasleft, only to be immediately forgotten; the leaves have fallen andthe reader is left unmoved. Although this kind ofreality appears in literary narrative, it remains a mere reality inreality, and not yet the reality in literature.

当火车经过以后不再回到我们的阅读里，当河边散步的人走远后立刻被遗忘，当树叶掉下来读者无动于衷，这样的现实虽然出现在了文学的叙述中，它仍然只是现实中的现实，仍然不是文学中的现实。

With the classic iodometric method,this paper can work out the total effective chlorine, the sum of the chlorine and chlorine dioxide in the system.

按通常的碘量法可测定体系的总有效氯(ClO2 +Cl2 )；向体系中加入一定量的掩蔽剂二甲亚砜后可有效地掩敝体系中的Cl2 ，进而可测定体系中的ClO2 。

By adding a few drops of dimethyl sulfoxide to mask the chlorine effectively,the concentration of chlorine dioxide was determined by general iodometric method.

按通常的碘量法可测定体系的总有效氯(ClO2+Cl2)；向体系中加入一定量的掩蔽剂二甲亚砜后可有效地掩敝体系中的Cl2，进而可测定体系中的ClO2。

Results The drug concentration in the abdoninal cavity was highst,the janitrix was secondary,the abdominal aorta and peripheral venous was the lowest .

结果：腹腔给药后药物在腹腔液中的浓度最高，其峰值浓度是外周静脉血中药物浓度的266.7倍，门静脉其次，约11.3倍，腹主动脉，外周静脉血浓度相仿，为最低。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。