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Fluorescence in situ hybridization was carried out to confirm the integration of HBV DNA into male pronucleus and its replication with cell division in embryonic development.(Reverse transcriptase polymerase chain reaction,RT-PCR) and immunofluoresence assay were performed to observe the expression of the HBV gene in two-cell stage.

材料与方法:成熟雄鼠麻醉后双侧睾丸注射经脂质体DOSPER包裹的HBV质粒,手术后雄鼠与超排雌鼠合笼交配,用荧光原位杂变(Fluorescence in situ hybridization,FISH)分别检测单细胞胚和二细胞胚间期核中HBV DNA的存在与复制,用逆转录聚含酶链反应(Reverse transcriptase-polymerase chain reaction,RT-PCR)和免疫荧光方法检测HBV基因在二细胞胚胎中的表达。

Recombinant expression vector k14/c-myc-paav was constructed bearing the mouse c-Myc cDNA derived by the skin specific human keratin 14 promoter, then the linear vector was microinjected into the two pronucleus of the C57BL/6 fertilized eggs to produce transgenic mice.256 microinjected eggs were transferred, 6 of the foster mothers were pregnant and 44 adult mice were produced, two of them were positive transgenic mice confirmed by PCR and Southern blot analysis, transgenic rate was about 4%.

以CMV-PAAV载体为基础载体,以皮肤特异人K14(人角蛋白14)基因启动子替换原质粒中的CMV启动子后,在其多克隆位点区依次插入鼠c-Myc基因的cDNA序列和gfp基因的编码序列,构建了高效表达c-Myc基因的真核表达载体。通过双原核显微注射技术,将线性化的载体DNA注入到C57BL/6小鼠受精卵的两个原核中,然后将注射后状态良好的胚胎移植到代孕鼠的输卵管中,共移植了256枚受精卵,6只受体怀孕,最终获得44只成年小鼠。

Firstly, it analyses the object of contract rescission from several angels, concludes that the object of contract rescission should be the original right and obligation in the contract, the contract itself and the right and obligation in the contract for the purpose of remedy is not rescinded. So the problem that whether contract rescission has retroactivity effect becomes the problem that whether contract rescission makes the original right and obligation in the contract vanishes retroactively.

笔者对合同解除的标的进行分析,认为合同解除的标的应该是合同内容中的原始性权利义务,合同解除后合同本身和合同中的救济性权利义务并不因合同解除而消灭,正因为此,关于合同解除是否有溯及力的问题也变成了合同解除后是否使合同中的原始性权利义务溯及地消灭的问题。

The obesity of Qu's figures is also suggestive. Overweight figures with stereotypic Chinese facial characteristics have expressively represented the insignificant multitude, and their intolerable mediocrity is an irony of the divinity they believe in. Such a sardonicism has existed in both the eastern and western worlds for a long time, but the artist did not merely appropriate it, instead, he transforms it .

另一方面,臃肿造型在瞿广慈作品中起了极大作用,臃肿且具有中国五官特色的人物是一种象征,是一中国人的标签,是将凡俗领域中的平庸推到极致的表现,是嘲笑神性领域至高性的无情揶揄;隐藏在这些丰厚人体后的尖锐讽刺,奠基于东西方历史中以存有的文本,但却不是单纯的印象复制,而是艺术家经过省思后的形式再造。

After the coefficients are quantized by a four-dimensional quantization matrix, most of them equal 0, and thus many zero planes appear in the submatrix.

用四维量化矩阵对变换后的四维子矩阵系数进行量化,量化后四维子矩阵中的变换系数大部分变成了零,这样就会在子矩阵中出现大量的零平面。

In the experimental tectum, the expression of GFAP in the right SO was higher than the expression of GFAP in the left SO during 1d-60d after after optic nerve damage. The expression of GFAP in the right SO was familiar with the expression of GFAP in the left SO during 85d after optic nerve damage, but the expression of GFAP in SO in the experimental tectum was higher than that in the normal tectum. The result of GFAP expression in the experimental retina and tectum showed that AStrocyte took part in the optic nerve regeneration and it might play an important role in optic nerve regeneration accidents.

在损伤1d后视网膜的神经纤维层和外核层外缘可见较为深的GFAP染色,在损伤3d后视网膜从内网层经内核层至外网层出现稀疏的垂直于视网膜长轴分布的线条状GFAP染色,随时间推移,伤后5d、7d、14d、28d上述染色进一步加深密集,到伤后60d和85d上述染色减弱,阴性对照的视网膜各层未见GFAP阳性染色;(5)正常鲫鱼视顶盖中在SO层内有较浅的黄棕色线条状GFAP染色,在SFGC层

A Some environmentalists are against the project on biological grounds.B What do the world's longest bridges have in common?D They're what will keep the bridge from going anywhere.E The second job will be to pull two sets of steel cables across the strait, each set being a bundle of 44,352 individuals steel wires.F They will tip up the scales at 166, 500 tons more than half the bridge's total mass.__2__. They're suspension bridges, massive structures built to span vast water channels or gorges. A suspension bridge needs just two towers to should the structure's mammoth weight,..

B。 分析:思路特点:注意空格后句中的词语使用特点:空格后面连续两句都涉及到了"suspension bridge/吊桥"因此判断空格处出现对"吊桥"进行讲述的句子的可能性最大,但被选项中没有该词出现,注意到空格后的句子中出现了代词They,该词的出现表明前面有复数的名词与之相呼应,而且They内容是指"桥",因此排除A,C和E, F(没有与They呼应的内容),所以答案是B。

A Some environmentalists are against the project on biological grounds.B What do the world's longest bridges have in common ?D They 're what will keep the bridge from going anywhere.E The second job will be to pull two sets of steel cables across the strait , each set being a bundle of 44,352 inPiduals steel wires.F They will tip up the scales at 166, 500 tons – more than half the bridge 's total mass.__2__. They're suspension bridges , massive structures built to span vast water channels or gorges. A suspension bridge needs just two towers to should the structure's mammoth weight,..

B。 分析:思路特点:注意空格后句中的词语使用特点:空格后面连续两句都涉及到了"suspension bridge/吊桥"因此判断空格处出现对"吊桥"进行讲述的句子的可能性最大,但被选项中没有该词出现,注意到空格后的句子中出现了代词They,该词的出现表明前面有复数的名词与之相呼应,而且They内容是指"桥",因此排除A,C和E, F(没有与They呼应的内容),所以答案是B。

A some environmentalists are against the project on biological grounds.b what do the world's longest bridges have in common?d they're what will keep the bridge from going anywhere.e the second job will be to pull two sets of steel cables across the strait, each set being a bundle of 44,352 individuals steel wires.f they will tip up the scales at 166, 500 tons – more than half the bridge's total mass.__2__. they're suspension bridges, massive structures built to span vast water channels or gorges. a suspension bridge needs just two towers to should the structure's mammoth weight,..

b。 分析:思路特点:注重空格后句中的词语使用特点:空格后面连续两句都涉及到了"suspension bridge/吊桥"因此判定空格处出现对"吊桥"进行讲述的句子的可能性最大,但被选项中没有该词出现,注重到空格后的句子中出现了代词they,该词的出现表明前面有复数的名词与之相呼应,而且they内容是指"桥",因此排除a,c和e, f(没有与they呼应的内容),所以答案是b。

RESULTS: Aggressie behaior was found in 3 patients (7.3%) among 41 patients with posterior cerebral artery stroke. One patient had right occipitotemporal and entrolateral thalamic stroke. The second patient had left occipitotemporal and lateral thalamic stroke. The third patient had right isolated occipital stroke.

结果:41例患有大脑后动脉卒中的病人中有3例(7.3%)发现有攻击性行为。1例患有右枕颞和丘脑腹外侧卒中,另1例患有左枕颞和丘脑外测卒中,第3例病人仅有右枕叶卒中。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?