后上

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

In the early morning, in a house-jacket, she looked after the management of her household. Then she drove on saints' days to Mass, and from Mass to the gaols and prisons; and of what she did there, she never spoke to any one. On ordinary days she dressed and received petitioners of various classes, of whom some sought her aid every day. Then she had dinner, an abundant and appetising meal, at which some three or four guests were always present. After dinner she played a game of boston; and at night had the newspapers and new books read aloud to her while she knitted.

大清早，她就穿上短棉袄，搞一点家务，之后，每逢节日去做日祷，日祷完毕后便去寨堡和监狱，她在那里从事什么活动，她不向任何人透露，在平日里，她穿好衣裳后，便来招待每天到她家里来的各个不同阶层的向他求援的人，然后用午餐，在味美而丰盛的午餐上，经常有三四位来客，在午餐之后打一圈波士顿牌，晚上叫人给她读报，给她读新书，她一边听，一边做针织活计。

This section presents two kinds of characteristic schemes, one is the natural use of the usual backward Euler characteristic difference scheme ( [60] ) onto the triangular mesh, the other replaces the backward Euler's method by modified Euler formula to approximate the characteristic differential equation, so that the discretization for characteristic curve can get up to second order accuracy.

本节给出了两种三角形网格上的特征差分格式，第一种是通常的向后Euler型特征差分格式（[60]）在三角形网格上的自然运用，第二种采用§3.3、§3.4的离散思想，使用改进的Euler公式代替向后Euler公式来近似特征微分方程，使其对特征线的离散达到了二阶精度。

The biomechanical tests showed that two kinds of artificial bones had not significant difference on compressive strength and Young\'s modulus(P＞0.05),while the flexural strength of nano-nacre artificial bone was less than the control group(P＜0.05).3.The results of CCK-8 showed that the difference were not significant in each group,the proliferation of osteoblast reached the peak at the 5th day;7 days after being co-cultured,the total protein content of study group was higher than control group and blank group(P＜0.05),while the difference between control group and blank group was not significantP＞0.05The difference of alkaline phosphatase activities among three groups was not significant(P＞0.05The SEM view showed that osteoblast attached and grew well in two kinds of artificial bone.4.X-ray photography showed that two kinds of powder started to degrade in 2 weeks;this phenomenon became more appear in 4 weeks,nano-nacre powder degraded faster than micron-nacre powder,while the hole shadow was easy to be found;in 8 weeks,all the femoral holes recovered and returned to normal bone mineral density in all groups.Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone grew fastest around the bone defect area in study group,while most slowly in blank groupP＜0.05 SEM(scanning electron microscope observation showed that nano-nacre powder degraded more quickly.The same result can be found through the demineralized sections morphometric analysis,and both of the composite artificial bones made from those two kinds of nacre powder had the good connection with the adjacent tissue in rats body without apparent inflammatory response.5.X-ray photography showed that rabbit\'s bone defects healed faster in study group since NNAB implanted than in control group since MNAB implanted.At 24 weeks after operation,bone density in radial defects had nearly accessed to the normal area,while lower in control group,and turned up nonunion in blank group;The checking of BMD showed that results in study group were higher than those in control group at 8,16 and 24 week(P＜0.05), and the difference between the BMD values in study group at 24 week and those in blank group was not significant(P＞0.05).The gross specimens showed satisfactory histocompatibility both in study group and in control group,with bone tissue growing from two sides into the center of implanted materials; Normal slices in HE stain and hard tissue grinding slices in Stevenel\'s blue/Van Geison\'s picro-fuchsin stain showed that the bone growth tendency was better in study group than that in control group,and the medullary cavity had been penetrated to the implanted materials in study group at 24 week;Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone in both groups grew fastest 8 weeks after surgery,while slow down at 16 week.

纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨分别与成骨细胞共培养后，其各时间点CCK-8法检测值与空白对照无显著差异(P＞0.05)，成骨细胞均在第5天达到增殖高峰期；培养7天后，实验组细胞蛋白含量高于对照组及空白组(P＜0.05)，后两者之间则无显著差异P＞0.05碱性磷酸酶活性在三组间均无显著差异(P＞0.05电镜下可见成骨细胞在两种人工骨上都有良好生长贴附能力。4.X-ray显示两种粉体在大鼠股骨骨洞植入第2周时都开始出现了降解，第4周时更为明显，纳米珍珠层粉较之微米珍珠层粉降解更快，而空白对照组骨洞阴影仍可见，至8周时，则所有组骨洞均己闭合修复，X-ray下已不可见原钻孔痕迹，恢复正常骨质密度；硬组织磨片四环素荧光双标记结果显示纳米珍珠层粉植入组较其余两组在骨缺损区周围新骨生长速度更快，空白组速度最慢P＜0.05电镜观察及常规脱钙切片亦可见到纳米粉体降解较快；由以上两种原材料制得的纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨在大鼠体内均与周围组织结合良好，无明显炎症反应。5.X-ray显示纳米珍珠层/消旋聚乳酸复合人工骨植入兔桡骨缺损区后其骨愈合速度较对照组微米珍珠层/消旋聚乳酸复合人工骨植入的快，至植入术后24周，实验组骨缺损区接近正常骨密度，对照组骨缺损区密度较低，空白组则呈现骨不连状态；骨密度测量结果显示术后8周、16周、24周实验组的骨密度值高于对照组(P＜0.05，24周实验组的骨密度值与术前所测得的正常值无显著性差异P＞0.05动物取材大体所见均显示组织相容性良好，骨组织逐渐由植入材料两端向中央生长；常规切片HE染色及硬组织磨片Stevenel\'s blue/Van Geison\'s picro-fuchsin联合染色均可见实验组骨缺损区长势优于对照组，至术后24周，实验组骨髓腔与材料已呈相交通状；硬组织磨片荧光显微镜下观察，两组材料在术后8周处于骨生长最快速时期，16周时速度开始减慢，术后4、8、16周时实验组的新骨生长速度均较对照组的快

And flat screen business card printing and membership card making, rotary screen business card printing and membership card is fixed in the making of squeege plates fixed drum inside of the card and membership card before making in the roller's internal full of ink, Business card printing and membership card when relying on print drums of turnused in the squeege of pressure so that the ink penetration into the production materials; business card printing and membership card making when you are finished, you can set by the membership card after cleaning and store up for once again business card printing and membership card when used.

与平网制卡和会员卡制作不对别的是，轮转丝网制卡和会员卡制作洋刮板是变动在印版滚筒本质不动的，制卡和会员卡制作后，不后在滚筒本质充斥油不朱，制卡和会员卡制作时凭借丝网版滚筒的回旋扭转，在刮板的压辛功用下使油不朱透印到承印质料上；制卡和会员卡制作达成后，可以不兵印版用响应的会员卡制作后摆设冲洗并储蓄起来，以备再不主制卡和会员卡制作时搁置。

The above production operations to undergo a rigorous control of detection, the ink attachment fastness of general usage requirements can be achieved, but because the milk membrane business card printing and membership card making, as printed on the table, that is, the surface of the ink in the film, is vulnerable to all kinds of injury, and water, acid, machinery, more or less friction will cause ink wash after wash, moreover, milk is in the filling is completed, are generally intended to be appropriated for cold storage of 4 ° c, milk from the inside out, the film surface as a result of alternating hot and cold, dew will be generated, in transit, bags and bags into as a result of vibration between, in the hands of consumers, and some of it will be on milk heating drinking, such a procedure requires ink has both a very good resistance to freezing, resisfance, thermotolerance and crocking performance and this envirogluvtm products in production is very difficult, so far, no inks contain all the characteristics of these four.

以上出产上各道工序不纬功澈底把持的检测后，油不朱的附着牢度凡是可以到达搁置请求，但由于奶膜的制卡和会员卡制作为边印，即油不朱在不厚膜的暗地，容难受到百般保养，火、酸、板滞、磨擦或多或少城市引起油不朱的掉色，况且，牛奶在灌装完不败后，凡是都需放出4℃的冷藏室冷藏，牛奶从冷藏室拿不出来后，不厚膜暗地由于冷冷瓜代，会爆不收结露不隐象，在输送途洋，袋与袋之间由于波动爆不收磨擦，到了出产者手边，有的还需差牛奶增冷饮用，这样一个功程不离请求油不朱同时具有不一不合精良的抗冷冻、耐火性、耐冷性和耐磨擦本能机能，而这样的油不朱产物在出产洋是不一不合艰难的，到目后为止，也操纵一栽油不朱能合座敷裕以上四栽共性。

Butt Weld and docking and corner combinations Weld, in Weld ends located arc plate and extraction plate in igniting board then leads to the welding seam, joint halfway shall weld joints in front of 2.0 mm Department Firing arc, welding pieces after preheating rod will return to weld the origin, pool filled to the required thickness, before forward welding; Welding speed : Isokinetic welding requirements to ensure weld thickness, width uniform, which masks to look for hot metal and slag bath equidistance (2-3 mm)%; Arc welding rod length : different models and determined that the general requirements of arc length is stable, acid electrode generally 3-4 mm, 2-3 mm normally alkaline electrode%; Welding perspective : According to determine the thickness of two pieces of welding, welding angle has two aspects : First, the angle between the direction of the rod and welding of 60-75 ; The second is the angle between two electrodes and welding around, the same thickness as welding, welding and welding pieces incident angle of 45 ; When the thickness ranging from welding, welding electrode and thick and thin pieces of welding electrode side angle greater than the angle between the side pieces; Arc : admission to the end of each weld should be filled crater, in the direction opposite to the direction of the back-arc welding, arc welding unchanged inside pit in 1974, to prevent the crater bite of meat.

对接焊缝及对接和角接组合焊缝，在焊缝两端设引弧板和引出板，必须在引弧板上引出后再焊到焊缝区，中途接头则应在焊缝接头前方15～20㎜处打火引弧，将焊件预热后再将焊条退回到焊缝起始处，把熔池填满到要求的厚度后，方可向前施焊；焊接速度：要求等速焊接，保证焊缝厚度、宽度均匀一致，从面罩内看熔池中铁水与熔渣保持等距离（2～3㎜）为宜；焊接电弧长度：根据焊条型号不同而确定，一般要求电弧长度稳定不变，酸性焊条一般为3～4㎜，碱性焊条一般为2～3㎜为宜；焊接角度：根据两焊件的厚度确定，焊接角度有两个方面，一是焊条与焊接前进方向的夹角为60～75 ；二是焊条与焊接左右夹角有两种情况，当焊件厚度相等时，焊件与焊件夹角为45 ；当焊件厚度不等时，焊条与较厚焊件一侧夹角应大于焊条与薄焊件一侧夹角；收弧：每条焊缝焊到末尾，应将弧坑填满后，往焊接方向相反的方向带弧，使弧坑甩在焊道貌岸然里边，以防弧坑咬肉。

Get away the beaker and put it on the board of the fuming hood when the reaction is over. Remove the leftover into the stainless steel basket till the beaker is cooled., open the tap when the wasted liquid has flowed away. Clean the chip with DI water, then it is dipped into acetone and clean with ultrasonic washer for 5 minutes.

反应结束后将烧杯从加热板上取下，冷却后把烧杯中的残留物倒入水槽中的不锈钢网篮中，待废液流净后打开水龙头进行冲洗，冲洗完毕后把腐蚀好的IC放到干净的烧杯中用去离子水清洗，然后向放芯片的烧杯中倒入适量丙酮进行超声清洗5分钟。

At the same time close the door of the fuming hood. Get away the beaker and put it on the board of the fuming hood when the reaction is over. Remove the leftover into the stainless steel basket till the beaker is cooled., open the tap when the wasted liquid has flowed away. Clean the chip with DI water, then it is dipped into acetone and clean with ultrasonic washer for 5 minutes.

反应结束后将烧杯从加热板上取下，冷却后把烧杯中的残留物倒入水槽中的不锈钢网篮中，待废液流净后打开水龙头进行冲洗，冲洗完毕后把腐蚀好的IC放到干净的烧杯中用去离子水清洗，然后向放芯片的烧杯中倒入适量丙酮进行超声清洗5分钟。

Get away the beaker and put it on the board of the fuming hood when the reaction is over. Remove the leftover into the stainless steel basket till the beaker is cooled., open the tap when the wasted liquid has flowed away. Clean the chip with DI water, then it is dipped into acetone and clean with ultrasonic washer for 5 minutes./ r '8 T9 A' \* r

反应结束后将烧杯从加热板上取下，冷却后把烧杯中的残留物倒入水槽中的不锈钢网篮中，待废液流净后打开水龙头进行冲洗，冲洗完毕后把腐蚀好的IC放到干净的烧杯中用去离子水清洗，然后向放芯片的烧杯中倒入适量丙酮进行超声清洗5分钟。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。