同质

Several inducers cicada exuviae, colloidal chitin, horsefly cuticle, shrimp, maggot and silkworm chrysalis were compared with their effects on production of cuticle-degrading protease of entomopathogenic fungus Metarhizium anisopliae.

研究了不同碳氮源对金龟子绿僵菌产生与分解昆虫外壳相关的蛋白酶活性和同工酶谱带的影响。结果表明：蝉蜕、虻虫壳、蝇蛆壳、蚕蛹壳、虾壳、胶体几丁质均可使金龟子绿僵菌产生蛋白酶，虾壳和胶体几丁质所产生的蛋白酶比活性最高，蝉蜕次之。

Lividans. Using pIJ903 bearing tsr gene as a vector, the two furthermost fragments of the cluster, 4. 0kb and 1. 5kb in size respectively, were inserted into it. To facilitate the detection of gene replacement, apr gene was placed in the middle of the two inserts. OriT from E coli plasmid RK2 was also incoporated into the vector, therefore, pIJ903 derivatives can be mobilized from E.

在具有硫链丝菌素抗性基因的pIJ903的单—BamHI位点，同向插入了FR-008生物合成基因簇两个最远端的4.0kb和1.5kb片段，并在二者之间插入了可在链霉菌FR-008和变铅青链霉菌中表达的阿泊拉霉素抗性基因，同时也插入了有助于将质粒引入链霉菌的oriT片段，从而构建出了置换整个基因簇的基因置换质粒pHZ691。

A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

However, GABA release could be laggardly evoked during the second 30 minutes by the venom, but not by BmK I.

结果提示，BmK I参予了东亚钳蝎粗毒诱发的脊髓背角内兴奋性神经递质的释放增加，与此相对应，抑制性神经递质GABA的释放增加可能同蝎毒中的抗伤害性成分有关。

The operating principle and performance of time-of-flight mass spectrometer were introduced.

介绍了飞行时间质谱仪的工作原理及性能特点、莱钢4号转炉应用的飞行时间质谱炉气在线分析系统的组成、安装布局及其同其他生产设备间的相互衔接，以及在现场达到的性能指标。

The research of recent years shows that phloem loading and unloading of plant photoassimilate both have symplasmic and apoplasmic pathways. Sugars transported by loading mainly are:(1) raffinose and its analogue,(2) sucrose.

近年来研究表明，植物体内光合同化物的韧皮部装载和卸出均有共质体途径和质外体途径，装载转运的糖类主要有：⑴棉子糖及其类似物；⑵蔗糖。

AIM: To explore the effect of homocysteine-mediated endoplasmic reticulum stress on lipid metabolism in hepatocytes.

目的：探讨同型半胱氨酸介导的内质网应激对肝细胞脂质代谢的影响。

Yang E,Korsmeyer SJ.Molecular thanatopsis:a discourse on the BCL-2 family and cell death.Blood,1996,88:386[3] Campos L,Rouanlt JP,Salido O,et al.High expression of BCL-2 protein in acute myeloid leukemia cells is associated with poor response to chemotherapy.

BCL-2是新一类癌基因——凋亡调控基因BCL-2家族的代表，其编码26kd的蛋白存在于线粒体、核膜及内质网，与同家族的其它成员组成不同比例的同、异二聚体而影响凋亡启动[6]。

Moreover, all the signals on X and Y only located in the heterochromatic regions, showing the same distribution with those of C-band.

所有X染色体上的信号是一致的，而X、Y染色体上的信号分布则彼此不同。X、Y染色体上的杂交信号区域均集中于异染色质区，信号分布模式同X、Y上异染色质分布模式完全吻合。

The properties of several rhodopsin sequences were analyzed by comparing withα2A-adrenergic receptor, and a great similarity was found between bovine rhodopsin andα2A-adrenergic receptor. Therefore transmembrane helices ofα2A-adrenergic receptor were constructed homologically based on the bovine rhodopsin.In the newly constructed structure, a 0.090 nm3 active-site, which was enclosed by several reported active residues, was found.

通过对比多个与α2A-肾上腺素受体同属G-蛋白偶联受体的视紫红质蛋白序列，选择以相似性最大的牛视紫红质蛋白为模板，同源模建了α2A-肾上腺素受体的跨膜结构，并在结构中找到了体积为0.090 nm3，已被报导的活性残基包围的活性位点。

The X-ray diffract was used to analyze the crystal structure of the composite. Results show that after the talc was treated with blend coupling agent, the crystal structure of talc changed, and effect Intercalation phenomenon.

通过X射线衍射分析可知，用混合偶联剂对滑石粉进行预处理后，滑石粉晶体结构发生改变，产生了插层现象，使PP／滑石粉复合材料为纳米复合材料。

An authentic Maui wedding might require the services of a 'kahu', which is a Hawaiian minister.

毛伊可能需要一个真实婚礼服务'kahu'，这是夏威夷部长。