同质

RESULTSdiabetic rats showed typical symptoms involving elevated serum glucose and weight loss (2)The rats in DM+VaD group reacted slower and made more error numbers in Y-maze than the rats in VaD group did, with a prolonged total reacting time in a whole day.(3)More neurons with serious damages, such as edema, ischemia or pyknosis could be found in DM+VaD group than in VaD group. More disarrangement of cone neurons, obvious glia proliferation, and more neuron apoptosis were found in CA area of hippocampus. CONCLUSIONS (1)2-VO in STZ-induced diabetic rats succeeded in establishing VaD animal models.(2) The cognitive dysfunction induced by VaD rats had been deteriorated by diabetes mellitus.(3)Morphology evidences proved that diabetes mellitus aggravated brain damages induced by VaD.PART II Effect of diabetes mellitus on the cholinergic nervous systemin CA1 area of hippocampus of VaDOBJECTIVE To estimate the role of cholinergic nervous system in hippocampus during the process of cognitive dysfunction aggravated by diabetes mellitus in DM+VaD group. METHODS Observe the changes of ChAT protein by immunohistochemistry stain. Chose three rats randomly from each group at each pointat two week, four week, and eight week, then sepa

经腹腔注射链脲佐菌素诱导慢性实验性糖尿病，1周后永久性结扎双侧颈总动脉(2-VO)制作VaD模型记录术后2周、4周和8周各组大鼠的体重及血糖；应用Y-迷宫检测空间定向学第二军医大学博士论文中…丈摘…要1ia；一色恤~﹁染澎糕行HE染色观察组织病理学孪叱；胶质细胞原纤维酸性蛋白protellZ，Cf冰尸标记观察星形胶质细舱的激活情况、橄声软公点娜育票票众糯黑橄居端粗默孺篇筑价井袱第二部分糖尿病对血管性痴呆大鼠海马c心区胆碱能神经系统的影响探讨海马CAI区胆碱能神经系统在糖尿病加重确D一大鼠认知障碍中所实验动物及分组同第一部分，应用免疫组织化学染色方法现察海马CAI{介狱牛第晕军l袭拔常博粗{论义

The pore capacity is becoming smaller with increasingthe vitrinite content. 2. Micro-porosity is dominated in the total pore capacity, which takes more than50%. There is not relationship among pore capacity and specific surface with otherfactors such as Maceral and R0, which shows that the distribution and shapes of poreare not influenced by the composition of the coal. 3. The pore is opened for the coal of Ordos Basin, the order is from durain to clarainthen samples to vitrain. Others are belong to half-opened and thin throat. 4. The adsorption content of gas is increasing with the higher pressure, and the freegas content is decreasing;The adsorption content of gas is decreasing with thehigher temp, and the free gas content is increasing;The adsorption content of gas isincreasing with higher vitrinite content for the kennel coal when temp.

总空隙主要由小孔孔容构成，小孔孔容占了50%以上；镜质组含量和惰性组含量等物质以及镜煤反射率均与孔容、比表面未表现出明显的相关性，说明孔隙分布特征不受物质组成的影响 3、鄂尔多斯地区的空隙呈现开放状态为主，空隙开放的次序为暗煤-亮煤-煤样-镜煤，其他盆地则为半封闭和细瓶颈空隙 4、随压力增加，煤对气体的吸附量增加，煤中所含游离气的量随之减小；随温度增加，煤对气体的吸附量减小，而煤中所含游离气的量随之增加；在相同的温压条件下，同是长焰煤的样品，吸附量在孔容中所占的比例随镜质组含量增加而增加。

Results: The tHey and blood fat level in blood plasma and expression of MCP-1mRNAof aorta on model group is obviously higher than that of control group (P＜0.05);the result of immunohistochemistry shows that the expression of VCAM-1 and MMP-2enhanced, Bcl-2 is lower; aorta slice shows endarterium discontinuation, strippingpartly, smooth muscle cell arrange puffly and chaotic, infiltration of lipoid ofdifferent degree are observed in aorta wall, pathological changes suffusion, morexanthoma cell, plaque protrude lumens. The they and blood fat level in blood plasmaand expression of MCP-lmRNA, VCAM-1, MMP-2 of aorta on intervention group aremuch lower than that of model group (P＜0.05); Bcl-2 of intervention group isstrengthened, and the pathomorphology and immunohistochemistry of aorta showpathological changes are slighter than model group.

结果：模型组，血浆中同型半胱氨酸浓度、血脂水平、主动脉MCP-1mRNA表达显著高于正常对照组(P＜0.05)，免疫组化示主动脉内皮细胞VCAM-1、MMP-2表达增强，Bcl-2表达降低；主动脉切片发现动脉内膜不连续，部分剥脱，平滑肌细胞排列疏松、紊乱，主动脉壁均见到程度不同的脂质浸润，其病变弥漫，泡沫细胞较多，可见斑块突出管腔；干预组，血浆中同型半胱氨酸含量、血脂水平显著低于对照组和模型组(P＜0.05)，主动脉MCP-1mRNA、VCAM-1、MMP-2表达显著弱于对照组和模型组(P＜0.05)，Bcl-2表达增强(P＜0.05)，主动脉病理形态学和免疫组织化学检测示病变轻于模型组。

On the basis of plan form and crosscutting relationship of the faults, two kinds of tectonic systems were developed in Wu-Xia area, one was strike north-east, and another was strike east-west, the first was developed early and control the structural framework of Wu-Xia area, after that three times longitudinal compress action was happed. According to the intensity of tectonic deformation, The Wu-xia faulted zone from north to south can be divided into the thrust faulted belt, the fault fold belt and the monocline belt. from east to west have three regulatory faults, Wuerhe fault fold belt, Wuxia thrust faulted belt and Xiahong fault fold belt. Base on that 9 secondary structural unit can be divided.Structural accommodation zone and the distribution of reservoir strata have intimate business connection. Usually, Structural accommodation zone was the source zone of arenaceous sediment. The Wu-xia faulted zone have 3 kinds of structural accommodation, concordant approach, concordant overlap and concordant colinearity.

首先研究本区的构造特征，根据断裂的平面形态及相互切割关系，乌夏地区发育两种构造体系：北东向构造体系和近东西向构造体系，前者形成早，控制了该区的构造格局，晚期有3次近南北向挤压作用叠加；乌夏地区南北根据构造变形的强度不同分为冲断带、断褶带、单斜带，东西由于横向调节断层的存在分为乌尔禾断褶区、乌-夏冲断区和夏-红断褶区，进一步划分为九个次级单元；构造调节带与储集层的分布关系密切，通常是大量富砂质沉积的源区，乌夏地区发育三种类型的构造调节带：同向接近型、同向叠覆型和同向共线型。

The characteristics of cryptocrystalline magnesite ore in Bameng Magnesite Mine were discussed.

论述了巴盟菱镁矿的特点，隐晶质菱镁矿多为热液交代型超基性岩类矿石，其基质致密坚硬，微细嵌布，杂质含量高，且有广泛类质同象，难以实现矿物单体解离，实际分选困难较大。

In other three groups, the left coronary artery was ligated to establish myocardial infarction models. Following 15 minutes of coronary artery deligation, the chest was opened. Rats in the cotransplantation group were treated with 250 μL (1×1010/L) BMSCs suspension + 8 mg/L Salvianolic acid B-treated EPC suspension 250 μL (1×108/L) in five points of the myocardium surrounding the deligation region. Rats in the BMSCs group received 500 μL BMSCs suspension. Rats in the model group were injected with an equal volume of cell medium.

结扎冠状动脉15 min后再次开胸，共移植组吸取骨髓间充质干细胞悬液250 μL(浓度1×1010 L-1)+ 8 mg/L丹酚酸B预处理的内皮祖细胞悬液250 μL(浓度1×108 L-1)，于结扎区附近心肌组织分5点注射；骨髓间充质干细胞组同法注射单纯骨髓间充质干细胞悬液500 μL，模型组注射等量细胞培养液。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Full-thickness articular cartilage defects (diameter: 3mm, depth: 3mm) were made to bilateral condylus medialis femoris of all rabbits. In periosteum+BMSCs group and periosteum group, defects were covered by homolateral autogenous proximal tibia periosteums, with germinal layer facing to cavitas medullaris. After that, the periosteum+BMSCs group received 3 sutures, followed by injection of 20 μL BMSCs suspension (1×109/L) into the defects, after which the last suture was taken.

在全部兔双侧股骨内侧髁造成直径3 mm，深度3 mm的全层软骨缺损，骨膜复合骨髓间充质干细胞组、单纯骨膜组取同侧胫骨上段内侧骨膜，骨膜生发层朝向骨髓腔覆盖软骨缺损，骨膜复合骨髓间充质干细胞组先期缝合3针，向缺损区注入1×109 L-1骨髓间充质干细胞细胞悬液20 μL，植入细胞后缝合最后1针。

In this section, the dissertation discovers that Zhu Fahu's Chinese-translated Buddhist Scriptures reflect that the Chinese language located in the period that ancient words and new words were exchanging and replacing each other rapidly. The vocabulary richness in Zhu Fahu's Chinese-translated Buddhist Scriptures is achieved by the way of compounds, synonym combination, tong-su-yi-xu synonymic words.

第三部分竺法护译经词汇的特点通过语词来源、构词和用词特点等几个方面，结合六组常用词相关的数据统计，指出竺法护译经词汇反映了魏晋南北朝时期汉语词汇处于新旧质替换共存的状态；竺法护译经中的词汇丰富和常用的表现方式通过复音化、同义连用、同素词、同素逆序词等几个途径实现。

Is the special production of clay, silica, calcium magnesium fire-resistant Materials, not stereotypes refractories and special refractory professional manufacturers, provinces and cities,"the contract benefits and trustworthy" unit, Green advanced units, clean factories, energy-saving advanced units, not stereotypes of China Institute of industry members of the unit…… no water aluminum company Carbonaceous pressure feed, HFPN-1 mud water cannons, iron-free baking ditch Ramming material at the country "on August 5,""9th Five-Year" in the key technology of the National Science and Technology Progress Award, the company in several years in a row National stereotypes refractories industry is not in the top competitions spears, over the years won the provincial and municipal QC achievement awards, the company and the various tertiary institutions, research centres all close relations of cooperation, to keep professional development trends, the timely development or transplant The latest research results and rapidly transformed into productive forces, the Company is a vigorous, effective high-speed operation of the entity, has been the development of refractories to play an active role.

淄博宏飞特种耐火材料有限公司是生产粘土质、硅质、镁钙质耐火材料、不定型耐火材料及特殊耐火材料的专业厂家，省市《重合同守信益》单位，绿化先进单位，请洁工厂，节能先进单位，中国不定型行业学会委员单位……公司的无水系铝碳质压入料，HFPN-1无水炮泥，免烘烤铁沟捣打料在国家&八五&&九五&科技攻关中获国家科技进步奖，公司连续数年在全国不定型耐火材料行业评比中名列前矛，历年荣获省、市QC成果奖，公司还与各大专院校、科研院所有密切的合作关系，随时掌握专业的发展动向，能够及时开发或移植最新的科研成果，迅速转化为生产力，本公司是一个富有朝气，运行高速有效的实体，一直为耐火材料的发展发挥积极作用。

The X-ray diffract was used to analyze the crystal structure of the composite. Results show that after the talc was treated with blend coupling agent, the crystal structure of talc changed, and effect Intercalation phenomenon.

通过X射线衍射分析可知，用混合偶联剂对滑石粉进行预处理后，滑石粉晶体结构发生改变，产生了插层现象，使PP／滑石粉复合材料为纳米复合材料。

An authentic Maui wedding might require the services of a 'kahu', which is a Hawaiian minister.

毛伊可能需要一个真实婚礼服务'kahu'，这是夏威夷部长。