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Experiments on decalcification and activation of osteoinductive ability, comparison between the osteogenesis of demineralized bone matrix in orthotopic and in heterotopic sites, and correlation between immune response to allograft of bone and osteoinductive activity of the graft, were reported in this paper. 1. Syngeneic deminerolized bone matrix and the mixture of DBM with bone ash or acid-soluble protein extracted from demineralization was implanted into muscle pouch of mice, subsequently, the inhibition of DBM osteoinduction by bone ash or acid-soluble protein was demonstrated with histological and by means of alkaline phosphatase assay.

本文对脱钙处理使骨诱导活性表达的作用、脱钙骨基质植入不同部位的成骨表现,以及同种异体骨移植引发的免疫反应与骨诱导活性的内在关系三方面进行了研究:(1)在小鼠肌肉植入同系脱钙骨基质及其与脱钙所去除的骨矿盐和酸溶性蛋白混合物,碱性磷酸酶和组织学检查表明:骨矿盐和酸溶性蛋白具有抑制脱钙骨基质骨诱导活性的作用。

The results displayed the symplasmic isolation of sieve element/companion cell complex from the surrounding parenchyma cells, and suggest that the photoassimilate unloading from phloem follows an apoplasmic pathway and must be facilitated via the sugar transporters.

由此可见,苹果果实韧皮部组织同周围薄壁组织存在共质体隔离,同化物必须通过糖转运蛋白的介导从筛分子以跨越质膜的方式卸出到质外空间,然后再装载到周围薄壁细胞中。

Result The medulla exponent of straight guard hair on corsac's back was 81.7%, and that on abdomen was 72.0%, the medulla exponent interval of hairs from 2 parts was 71.4 %-82.9%, indicating the medulla exponent of animals in same family had a certain difference.

结果]沙狐背部直针毛的髓质指数为81.7%,腹部为72.0%,2个部位的髓质指数区间为71.4%~82.9%,说明同科动物的髓质指数存在一定的差异。

This paper establishes mathematical models of unsteady and Non-Darcy low speed flow through the porous media and the double porous medium, and has obtained their solutions in an infinite reservoir through Laplace transformation, and their long time asymptotic solutions has been worked out, solutions on a bounded reservoir has also obtained through finite integral transformation and Laplace transformation.

从现场上各种水淹的实际资料来看,同一水动力的非均质油层系统中,渗透率相对较高的部分水洗之后,渗透率相对较低部分的油层却可以原封不动。而且,其渗透率可达达西级,含油饱和度可达80%以上。相对流动比(即同一水动力的非均质油层系统中,小层平均渗透率的最大值与其中不流动部分的小层平均渗透率的最大值相比)的比值在各种油层的生产实例中十分相近。一般的分布范围为3—7,常见的为4—6。实验室结果和生产实践基本相同。简单的注水和压裂对这一比值几乎无影响。

In every group, rabbits were subdivided into experimental and control subgroups. 2 Rabbits were bullets injected followed by continuous injected with 13C labeled leucine, glucose, and lactic acid; 3 Blood were drewed before and 150, 160, 170, and 180 min after the initiation of isotopes injection for material analysis; 4 Exhale gas were collected every 5 min in the first 30 min followed by every 30 min there after for material analysis; 5 After centrifuge in low temp the supernatant of the blood samples were collected and went through axon and anon exchange column treatment; 6 Treated blood sample was used for 13C labeled leucine, glucose lactic acid examination through mass spectrograph; 7 The exhale gas was collected for 13CO2 exam through gas-phase mass spectrograph; 8 CO2 total production rate (V13CO2), body various substances production, oxidation speed, and substances metabolic percentage all can be calculated through equations provided by references below; 9 Data was processed through student t test.

分亮氨酸、葡萄糖和乳酸三组各取健康新西兰兔16只,每组再分为高代谢脓毒症组和对照组两部分,建模方法同第一部分;2)三组分别先静脉弹丸式推注再持续灌注13C标记的亮氨酸、葡萄糖和乳酸;3)灌注前抽取动脉血作为背景值,灌注达150,160,170和180min分别抽取动脉血2ml用于质谱分析;4)实验开始后前30min中每5min收集呼出气一次,随后每30min收集呼出气一次,用于气相质谱分析;5)血样经过低温离心取上清液,过阳离子及阴离子交换树脂,再经衍生化处理;6)处理过的血样进入气相色谱-质谱仪,测量其13C标记的亮氨酸、葡萄糖和乳酸丰度;7)实验兔的呼出气通过13CO2气相质谱分析仪测定其中的13CO2丰度(E13CO2);8)利用文献提供的公式算出CO2总生成率(V13CO2)以及机体各物质的通量、氧化速率以及物质代谢百分比等;9)数据分析处理采用t检验。

From the femur bone density analysis of protein expression, we can see that light chain lactoferrin, annexin decreased and the expression of enolase, ATP synthase, acetyl-coenzyme A reductase, the expression of troponin, may be related to ovariectomized femoral bone density in osteoporosis-related happened; strong bone of the femoral osteoporosis Po intervention effect may be related to reduced enolase, ATP synthase, troponin, CK-MB, acetyl phosphoglycerate enzyme, the expression of myosin, and the increase in lactoferrin light chain, pyruvate kinase isoenzyme, protein expression crown.

从股骨密质骨蛋白质组表达分析可知,乳铁蛋白轻链、膜联蛋白A3的表达下降及烯醇化酶、ATP合酶、乙酰辅酶A还原酶、肌钙蛋白的表达增强,可能与去卵巢后股骨密质骨骨质疏松的发生相关;强骨宝对股骨骨质疏松的干预效应可能与下调烯醇化酶、ATP合酶、肌钙蛋白、肌酸激酶同工酶、磷酸甘油酸变味酶、肌球蛋白的表达,和上调乳铁蛋白轻链、丙酮酸激酶同工酶、冠蛋白的表达有关。

The Chitinase of Saccharomyces cerevisiae(CTS1-2) is an Endochitinase which is endogenous in Saccharomyces cerevisiae. Its recognition and slipt site lies in the β-1,4 glucosidic linkage of homopolymer. Included in the amino acid sequence from N-terminal to C-terminal of the Saccharomyces cerevisiae chitinase are signal peptide sequence, catalytic domain, binding domain and a C-terminal shorten region.

酿酒酵母几丁质酶(Chitinase,CTS1-2)是一种来自酿酒酵母的内切几丁质酶,它的酶切位点位于其同聚物内的β-1,4连接的糖苷键,酶蛋白分子结构从N端到C端依次为信号肽、几丁质催化区、几丁质结合区以及一个短的C末端区。

Recombinant plasmid pSVH 7 DNA of avian influenza virus H7 subtype heamagglutinin gene was encapsulated with DC-chol/DOPE liposomes and PC/chol/SA liposomes separately. Two-week old SPF chickens were intramuscularly inoculated with 50 μ g/0.2ml of the liposome entrapped PSVH 7 DNA. Four-weeks later, each chicken was challenged with 0.1ml 〓 AIV . One week after the challenge, the secretion of the cloacas was collected and transfected to chicken embryos to isolate the virus. The virus was isolated from 6/6 of the control group, 1/6 of the naked DNA group, 1/6 of the PC/chol/SA entrapped DNA group and 0/6 of the DC-chol/DOPE liposome entrapped group. The HI antibody titers (log2) of the four groups were 6. 83±0.98, 7. 0±1. 26, 7. 83±1. 17 and 8. 00±0.89 respectively 1-week after challenge, and 8. 5±0.55, 8. 17±0.82, 8. 68±0.45 and 9. 33±0.54 respectively 2-week after challenge. The results showed that inoculation of liposome entrapped DNA significantly enhanced resistance to virosis in animals.

将含禽流感病毒H7亚型血凝素基因的重组质粒pSVH7用DC-chol阳离子脂质体和胆固醇/卵磷脂/十八胺脂质体包裹,免疫2周龄SPF鸡,4周后用同型禽流感病毒进行人工感染,1周后采集泄殖腔分泌物分离病毒,结果未免疫组6/6分离到病毒,裸质粒DNA免疫组1/6分离到病毒胆固醇/卵磷脂/十八胺脂质体包裹DNA免疫组1/6分离到病毒,DC-chol脂质体DNA免疫组没有分离到病毒(0/6):人工感染后1周各组的HI抗体(Log2)分别为6.38±0.98,7.00±1.26,7.83±1.17,8.00±0.89,2周后为8.50±0.55,8.67±0.82,8.68±0.45,9.33±0.52,脂质体包裹组在同期均高于未免疫组和裸DNA免疫组,表明脂质体包裹质粒DNA免疫动物后,能增加动物对病毒感染的抵抗力和反应能力。

Pristine Li-Al LDHs are synthesized by hydrothermal process in different reaction conditions by varying the aging time for 1 and 24 hours. Both the Li-Al layered double hydroxides (abbreviated as Li-Al LDH1 and Li-Al LDH24 for aging time 1 hour and 24 hours, respectively) were modified by using sulphanilic acid sodium salt hydrate, modified agent to form modified Li-Al LDH1-SAS and Li-Al LDH24-SAS. The morphology of the pristine layered double hydroxides are investigated by using wide angle X-rays diffraction, scanning electron microscopy and particle size analyzer which indicates that the crystallinity and aspect ratio is increased with increasing the aging time from 1 hour to 24 hours. The particle size of Li-Al LDH24 and Li-Al LDH1 are found to be ~1000 nm and ~250 nm, respectively. Both the pristine and modified LDHs are characterized by XRD, FTIR spectra and thermogravimetric analysis.

本研究目的在於改变长晶时间的长短,合成出同径大小的Li-Al LDHs,再经由改质剂sulphanilic acid sodium salt hydrate将Li-Al LDHs无机层材表面有机官能化制备出改质型Li-Al LDHs-SAS,於是进一步藉由扫描式电子显微镜、射径仪和穿透式电子显微镜等仪器分别观察LDHs改质前和改质后其主层结构型态上的变化;从中可以发现Li-Al LDHs随著长晶时间的增加其径有随之增大的趋势,但经有机化改质后其径会由於改质环境的影响明显低许多,针对此现象本实验将未改质和改质后之Li-Al人工无机层材制备成复材进一步探讨其在热性质和难燃特性上的为表现。

When aborted rats in group E were treated with the dose of 50 IU/g IFN-γ, expression of TNF-αin this group was compared with the other four groups, the results were as follows: Compared with group A, the nucleus pre-opticus medialis, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami and pars intermedia had a highly expression of TNF-α, in the other nuclei of hypothalamus, neurohypophsis and adenohypophysis, the change was not significantly, the expression of TNF-αin follicle and corpus luteum were decreased distinctly, the variation in uterus was slight and no difference was exsited; Compared with group B, the expression of TNF-αin nucleus nucleus lateralis hypothalami, nucleus ventromedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis and uterus presented a remarkable enhancement, conversely, the TNF-αexpression in the nucleus pre-opticus medialis, nucleus pre-opticus suprachiasmaticus, nucleus supra-opticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, neurohypophsis, follicle and corpus luteum had been significantly decreased; Compared with group C, the nucleus pre-opticus medialis, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis, pars intermedia and uterus had upregulated the expression of TNF-α, however, it was decreased in the nucleus periventricularis hypothalami and corpus luteum; When compared with group D, the expression of TNF-αin nucleus lateralis hypothalami, nucleus dorsomedialis hypothalami, nucleus ventromedialis hypothalami, pituitary, follicle and ovary stroma were obviously enhanced, while in the nucleus supra-opticus and nucleus arcuatus hypothalami, the situation was definitely opposite, in the other parts, the change was not significant.

外源腹腔注射50 IU/g IFN-γ后,同A组相比:E组TNF-α免疫阳性产物在下丘脑视前内侧核、视前大细胞核、室周核、腹内侧核、背内侧核、弓状核、垂体中间部显著增高,在下丘脑其它核团、神经垂体、垂体前叶较A组差异不显著,在卵泡、黄体中阳性产物表达均较A组显著降低,在子宫中表达较A组变化不明显;同B组相比:E组TNF-α免疫阳性产物在下丘脑外侧核、腹内侧核、弓状核、垂体前叶、子宫均显著增高,在视前内侧核、视交叉上核、视上核、视前大细胞核、室周核、室旁核、神经垂体、卵泡、黄体中阳性物质表达均显著降低;同C组相比:E组下丘脑视前内侧核、腹内侧核、背内侧核、弓状核、垂体前叶与中间部、子宫均显著增高,在室周核、黄体中均显著降低;同D组相比:E组下丘脑外侧核、背内侧核、腹内侧核、垂体各部、卵泡、卵巢基质均显著增高,在视上核、弓状核显著降低,在其它部位表达变化差异不显著。

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