同种移植

Methods Thirty SD rats and 60 Wistar rats were randomly divided into isograft group as control (donors and recipients were both Wistar rats) and allograft group (the SD rats as donors, Wistar rats as recipients).

将SD大鼠和Wistar大鼠随机分为同种同基因组和同种异基因移植组。

When the porcine-porcine and porcine-mouse nuclear transfer embryos were activated,inthe former only one pronucleus was observed in most cases,but in the later two pronuclei werefound mostly.

猪-猪同种核移植胚胎和猪-小鼠异种移核胚胎激活后，异种重组胚多形成二原核(2PN)，而同种重组胚则主要形成一原核。

ObjectiveTo assess the relationship of the expression of bcl-2/bax and the acute rejection of small intestine transplantation ,And open up news pathway for diagnosising acute rejection of small intestine transplantation in earlier period-MethodsCreated 36 rat model of allogene small intestine transplantation by using inbred rat Wistar/A and F344/N.

目的 建立同种异基因大鼠小肠移植模型，检测术后移植小肠组织内凋亡基因Bcl-2和Bax的表达情况，探讨其与急性排斥反应之间的关系，为临床早期诊断急性排斥反应开辟新途径。方法选用近交系F_(344)／N和封闭群Wistar／A大鼠建立全小肠移植模型。

The substitute of bone has autogenous bone, allgrafts, xerografts and alloplastic materials four big classes of bone transplanting.

骨替代物有自体骨移植、同种之间骨移植、异种之间骨移植、人工合成骨粉四大类。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Induction of isotypic heterostrain antibody (Ab1): BALB/c mice were immunized with C57BL/6 splenocytes to raise antibody (Ab1) between the two difference strains.

抗同种异品系抗体(Ab1)的诱导 C57BL／6小鼠作为移植供体，BALB／c小鼠作为受体。取C57BL／6小鼠的脾细胞免疫BALB／c小鼠，制备抗同种异品系抗体(Ab1)。

［Objective］To compare the clinical results of different bone graft in the posterior route spinal fusion of adolescent idiopathic scoliosis.［Method］A retrospective study was made in 71 case of adolescent idiopathic scoliosis,who were operated on with posterior instrumentation of screw-rod fixation system from 2000 to 2005.All of the patients were divided into three groups,autologous bone graft in 21 cases,allogous bone graft in 23 cases, autologous bone mixed allogous bone graft in 27 cases. The fusion rate, the Cobb's angle loss rate of incidence and pseudoarthrosis were compared among the three groups at 3,9,15,36 months follow-up.

［目的］通过不同植骨在青少年特发性脊柱侧凸后路矫形植骨融合术中的应用比较，探讨其临床应用效果［方法］回顾性分析2000年～2005年行后路钉-棒系统矫形手术的71例青少年特发性脊柱侧凸患者，其中自体骨移植21例，同异体骨移植23例，自体骨混合同种异体骨移植27例，比较随访第3、9、15、36 月的融合率、Cobb's角丢失率及假关节发生率。

To minimize complications from urine reservoir constructed with an intestinal segment,reticular transplantation of bladder mucosa to an intestinal seromuscular segment was conducted in 3 randomized groups of canines.

为减少肠管膀胱术的并发症，于1994年3～7月，用犬分三组作了膀胱粘膜点状移植于浆肌层肠段重建膀胱的实验。A组：自体膀胱粘膜点状移植于浆肌层肠段形成人工膀胱，B组：在A组基础上一期将输尿管与人工膀胱吻合，C组：将同种异体膀胱粘膜移植于浆肌层肠段。

Drill of cross pin system was used to make defects vertically to cartilage face at condylus medialis femoris of left knee joint in rabbits.

用横穿钉钻头在兔左膝关节股骨内侧髁垂直于软骨面制造缺损，缺损深4 mm，直径4.45 mm，自体移植组取右膝关节内侧髁骨软骨柱植入到左侧膝关节相应缺损处，异体移植组将经过程序性深低温冷冻保存的同种异体骨软骨柱移植到相应缺损处。

It was found that in the group of allogra fts intragraft IL-2R and ICAM-1 were expressed on the POW 4 and peaked on the POW 32, and their expression levels were significantly higher than in the group of autografts and isografts.

结果发现同种异体肾移植组于第4周开始出现IL-2R及IC AM-1的表达，于32周达高峰，明显高于自体肾移植组和同质移植组。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。