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In addition, some modifications on several computational methods are also presented. Using LMTO method the electronic structure of several systems are studied, and some results are obtained. They are: The ideal Nb (100) surface has three surface states, the multi-layer relaxed surface has two surface states. The surface energy of the ideal surface is higher than that of the relaxed surface, that means that the multi-layer relaxed surface is more stable than the former one, which supports the LEED results. The mono-layer relaxed Ag (111) surface is the most stable one among several" stable surface models"presented by several researchers. The surface energy of Ag (111) surface is higher than that of surface Ag (001), which supports some experimental results such as different reaction rate at different surface orientations for the same material. The surafce states of Si (111) surface not only locate near the Fermi level, but also in the valence band, which agrees well with Cohen's conclusion. Si (111)-H is an effective model for analysing the surface states and H adsorbed on the back surface is a good method for improving the convincingness of the results obtained on thinner slab models. The surface stability depends on three different kinds of MoSi〓(001) surfaces, the surface with mono-layer Si is the most stable one, and the surface with Mo at the first layer is the most unstable one among them. These are consistant with the Kemoda's experimental results. The valence bands of clean or K adsorbed CdTe (111) surface agrees well with the synchrotron radiation studies. The surface of CdTe (111) consists of four kinds of surface models which show different surface electronic structures and different surface structure stabilities. The conclusion agrees well with Wu's experimental work. The different absorbed alkali metals on the CdTe (111) surface give different adsorption characteristics which have relations not only with the valence electrons, but also with the core ones of the alkali metals. The electonic structures of Si-C alloys are different from that of Si-Ge alloys, and the energy band gaps of Si-C alloys do not increase linearly with Carbon concentration, our conclusion supports Alexander's results, but conflicts with Soref's one.

现分述如下: LMTO方法及其应用方面:1)通过对Nb(100)表面电子态分析发现清洁理想表面有三个表面态,多层弛豫表面有两个表面态;表面能大小说明多层弛豫表面更稳定,支持了LEED结果。2)通过对采用不同方法获得的几种不同Ag(111)表面稳定结构的表面能计算分析,给出了单层弛豫表面为Ag(111)表面的最稳定结构;从Ag(111)单层弛豫表面和Ag(001)表面的表面能比较,发现了Ag(001)表面表面能要比Ag(111)小的,表明了同种物质不同表面取向将表现出不同物理、化学性质,这是与实验中得出的结论是吻合的,3)通过对Si(111)表面态分析,不仅发现了Si(111)表面不仅具有居于费米能级附近的悬挂键所对应的表面态,而且还有很多表面态位于价带能量范围内,与Cohen等结果一致,H饱和slab模型背表面相当于增加了slab层的厚度,是一有效的变相增加slab层厚的方法,弛豫表面较清洁理想表面价带谱们低能端的少许移动,预示着总能降低,说明弛豫表面较清洁理想表面稳定。4)MoSi〓具有三种表面,从费米能级上态密度值大小得到单层Si表面最稳定,Mo原子为表层原子的表面最不稳定,双层Si原子表面居中的结论,这与Kemoda等人实验结果是一致的。5)通过对CdTe(111)表面表面电子态、表面结构稳定性及表面H、碱金属吸附的电子结构系列研究,不仅得出了CdTe(111)清洁及碱金属K吸附价带谱与同步辐射光电子谱相吻合的结果,而且发现了CdTe(111)表面具有四类不同原子近邻特征,表现出四类不同的表面结构及电子结构特征:不同表面态分布、不同的表面结构稳定性(表层原子与次层原子成三键有一悬挂键的表面要比表层原子与次层原子成一键有三悬挂键稳定(与Wu等人实验结果一致))、不同的H吸附特性。

Pass to count to Wisterch parameter , and the steel wire is different from the molding tool get in touch with the length, and settle the path the research taking the length work the stabber angle to get under differently pull to stir the term, mold core adoption of best severally why size, combine the best work stabber of research the Cape, and settle the path to take the length nextly steel wire is in the molding tool of have fever to measure, air inside dispel-heat calories, winding dispel-heat on the coil-canister , and its molding tool dispel-heat calories of, and cause the steel wire the temperature difference pass in and outing the molding tool to influence steel wire machine function, under the experiment inside pull to stir the term according to the same race different work stabber Cape half of adoption the Cape(7 degree,10 degree,12 degrees, the best mold Cape, experience mold Cape) settle the path to take the length(2-3 mm,3-4 mm,4-5 mms, theories length, experience length) to proceed to experiment, and get the conclusion, work stabber the Cape then is more big the steel wire get in touch with the length with

通过对Wisterch参数,钢丝与模具接触长度、定径带长度、工作锥角度的研究得到了在不同拉拨条件下模芯采用的最佳几何尺寸,并研究最佳工作锥角、定径带长度下钢丝在模具中的发热量、空气中散热量、卷筒上的散热量及其模具散热量的不同,导致钢丝进出模具的温度差别对钢丝机械性能的影响,在实验中根据同种拉拨条件下采用不同工作锥角半角、定径带长度进行试验,得出结论,工作锥角越大则钢丝与模具接触长度越短,钢丝变形越剧烈,在模具中发热量就越高,导致钢丝出模温度高,使钢丝强度、硬度升高,弯曲、扭转次数降低,并且降低模具使用寿命。在其它条件相同的情况下,定径带越长则钢丝与定径带简壁磨擦增大,使钢丝发热量加大,从而使钢丝强度、硬度略升高,弯曲扭转次数略降低但定径带的增大减小了钢丝变形产生的弹性后效使钢丝出模直径比较稳定。

The rules of photo bioactivities are: Without illumination, the tested xanthene derivatives showed almost the same level of inherent toxicity to the same organism, which showed the inherent toxicity of xanthene derivatives were primarily dependent on the structure of parent molecule. Upon illumination, the photosensitizers showed obvious phototoxicity to all organisms. The xanthene derivatives showed stronger phototoxicity to Gram-positive bacteria. With the increasing number of halogen substituents, the singlet oxygen yields increased and the phototoxic activity increased too.

呫吨类化合物的生物活性的变化规律为:未经光照的条件下,呫吨类化合物的对同种待测微生物的固有生物活性基本相同,说明固有毒性可能主要决定于化合物分子的母核结构,其卤素取代基团对其影响较小;经实验光源照射下,所测MLC值明显低于未经光照条件下的值,表明呫吨类化合物对三种微生物的光敏活性明显强于对其相应的固有活性;呫吨类化合物对革兰氏阳性菌能发挥较大的光敏活性。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC;分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态;流式细胞术检测DC表型;HRP吞噬实验测定DC的群体内吞能力;MTT法检测同种异体混合淋巴细胞反应;ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平;冻融法制备肝癌细胞可溶性抗原;流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性;MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响;SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度,ELISA测定活性;流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型;MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应;流式细胞术检测肝癌细胞表面HLA-DR表达;MTT法检测肝癌DC疫苗对自身淋巴细胞的活化;原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达;流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L;MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用;Cell-ELISA检测人肝癌细胞hAFP表达;MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响;ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平;肝癌细胞凋亡的诱导和检测;DC吞噬凋亡肝癌细胞后的电子显微镜观察;DC对肝癌细胞的生长抑制试验;人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定;DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤;冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

In addition this paper,"Bio-Rad method," two parasitic wasps of the post-embryonic development the process of protein accumulation in young bees and the host Plutella xylostella in this process in vivo haemolymph protein content were measured and found that over time, was gifuensis parasitic larvae of the diamondback moth haemolymph protein content showed an upward trend in nearly a straight line, Ichneumonidae parasitic For example, in the early parasitic diamondback moth haemolymph protein content also showed the trend of slow growth, but in the mid-and late parasitic young bee about to be completed in the development of the host body when to stop feeding, a significant downward trend却呈, and significantly lower than the third day of its own protein content; on the same day of the different species of the diamondback moth parasitic wasp hemolymph protein content compared Ichneumonidae Parasitism of diamondback moth have hemolymph protein content than in the third and fourth day after the parasitic Braconidae hemolymph content to be high, but very different in the case of the fifth day, as evidenced by her side after the parasitic bee moth haemolymph protein content is cocoon of the parasitic bee hemolymph protein content of 0.7 times.

另外本文采用&Bio-Rad法&对两种寄生蜂胚后发育过程中幼蜂蛋白积累及寄主小菜蛾在此过程中体内血淋巴蛋白含量进行了测定,结果发现随时间的推移,被茧蜂寄生小菜蛾幼虫的血淋巴蛋白含量是呈近直线增加趋势,而对于姬蜂寄生来说,在寄生初期小菜蛾血淋巴蛋白含量也呈缓慢增长趋势,但在寄生中后期,幼蜂即将完成在寄主体内的发育取食停止时,却呈大幅下降趋势,且明显低于其自身第三天的蛋白含量;对同一天的被不同种寄生蜂寄生的小菜蛾血淋巴蛋白含量进行比较,姬蜂寄生过的小菜蛾血淋巴蛋白含量在第三天和第四天均比茧蜂寄生后的血淋巴含量要高,但在第五天的情况大不相同,表现为被姬蜂寄生后的小菜蛾血淋巴蛋白含量是被茧蜂寄生的血淋巴蛋白含量的0.7倍。

An antibody binding to IGF-IR and inhibiting the binding of IGF-I and IGF-II to IGF-IR which is characterized in that said antibody is a is ofIgG1 isotype, b shows a ratio of IC50 values of inhibition of the binding of IGF-I to IGF-IR to the inhibition of binding of IG-II to IGF-IR of 1:3 to 3:1, c inhibits for at least 80% at a concentration of 5 nM IGF-IR phospohrylation in a cellular phosphorylation assay using 3T3 cells providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0.5% heat inactivated fetal calf serum when compared to such an assay without said antibody, and d shows no IGF-IR stimulating activity measured as IGF-IR phophorylation at a concentration of 10 M in a cellular phosphorylation assay using 3T3 providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0,5% heat inactivated fetal calf serum when compared to such an assay without said antibody has improved properties in antitumor therapy.

一种已经提高了抗肿瘤治疗的特性的抗体,所述抗体结合IGF-IR并且抑制IGF-I和IGF-II与IGF-IR结合,其特征在于所述抗体a是IgG1同种型,b显示其对IGF-I与IGF-IR结合的抑制的IC 50 值与其对IGF-II与IGF-IR结合的抑制的IC 50 值的比率为1∶3-3∶1,c当与没有所述抗体的这种测定比较时,其在5nM的浓度上,在包含0.5%的热灭活胎牛血清的培养基中,使用3T3细胞的细胞磷酸化测定中,抑制至少80%的IGF-IR磷酸化,所述3T3细胞提供400,000-600,000分子IGF-IR/细胞,和d当与没有所述抗体的这种测定相比,在包含0.5%的热灭活胎牛血清的培养基中,使用3T3的细胞磷酸化测定中,其在10μM的浓度上没有显示作为IGF-IR磷酸化所测量的IGF-IR刺激活性,所述3T3提供400,000-600,000分子IGF-IR/细胞。

The article begins with the establishment of the ETUR and its disputation, argues the rationality of the use of explanation context here, then analyses the different explanations of the ETUR and educes three different explanation contexts, as epistemological, ontological and semasiological, considering the reference, meaning and its extended meaning in quantum mechanics as the elements of explanation context.

文章首先从能量-时间不确定关系的提出、关于其的争论等入手,论证了对其作解释语境考察的合理性,而后基于物理学家们所做出的不同种解释进行分析归纳,指出△E和△t的指称、△E·△t≥/2的意义及其意义在量子力学中的延伸作为解释语境的构成要素共同构成了对于ETUR的三种不同解释语境,即认识论的解释语境、本体论的解释语境、语义学的解释语境。

The markets are just as kind to other euro-zone spendthrifts. Government bonds from Greece (deficit last year, 5.2% of GDP; debt, 108%; but GDP growth, healthy) yield about the same as Italy's. Those from Portugal (deficit, 6%; debt, 63%; growth, perkier this year after a feeble run) yield ten basis points less.

希腊(去年的赤字占到该国GDP的5.2%,债务占到GDP的108%,但是该国GDP的增长健康)公债收益率和意大利同种公债的收益率相同,而比葡萄牙(赤字占到该国GDP的6%,债务占到GDP的63%,在经历了缓慢的增长后,今年该国的经济增长会更有强劲)低10个基点。

Sets of kingbirds' eggs, eight sets of house wrens'eggs, four sets of mockingbirds' eggs, etc.; sets ofeggs taken in low trees, high trees, medium trees;spotted sets, dark sets, plain sets, and light setsof the same species of bird. Many collections aremade on this latter plan.

我听说,一个私人的收藏品包含12窝必胜鸟的蛋,8窝莺鹪鹩的蛋,4窝仿生鸟的蛋,等等;那些成窝成窝的鸟蛋都是在低矮的树、高树、中等型的树上被截获的;是一窝窝同种鸟类的蛋,有斑点的,黑色的,无花纹的和浅色的。

The village had formerly contained, side by side with the agricultural labourers, an interesting and better informed class, ranking distinctly above the former - the class to which Tesss father and mother had belonged - and including the carpenter, the smith, the shoemaker, the huckster, together with nondescript workers other than farm-labourers; a set of people who owed a certain stability of aim and conduct to the fact of their being life-holders like Tesss father, or copyholders, or, occasionally, small freeholders.

从前在乡村里,还有另外一个有趣的、见识广的阶级同种地的庄稼汉居住在一起,他们的地位比庄稼汉高,苔丝的父亲和母亲属于这个阶级,这个阶级包括木匠、铁匠、鞋匠、小贩,还有一些除了种地的庄稼汉而外的不好分类的人。他们这一班人都有固定的目的和职业,有的和苔丝的父亲一样,是不动产的终身所有人,也有的是副本持有不动产的人,有时候也有一些小不动产所有人。

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The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性,因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊,这是第二次你打败了我!

So a kind of strong antagonism permeated in the relations of two countries.

所以两国这一时期的政治关系始终弥漫着一种强烈的对抗情绪。