同种异体的

But the role of allograft and xenograft as carriers of rhBMP-2 in spine arthrodesis wasn't reported and reserved exploring

但是同种冷冻干燥异体骨和异种冷冻干燥骨做为rhBMP-2的载体在脊柱后外侧融合方面的作用鲜有人报道，值得探索。

Before long it developed into two more complicated forms: homograft and heterograft .

不久，自体移植术又发展为两种较复杂的形式：同种移植术和异体移植术。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

The purpose of this study was to prospectiely analyze the clinical outcome and graft morphology of patients who receied fresh, hypothermically stored, allograft tissue for the treatment of symptomatic chondral and osteochondral defects of the knee.

这项研究的目的就是前瞻性分析接受用新鲜低温冻存的同种异体骨组织治疗有症状的膝关节软骨和骨软骨缺损的病人的临床疗效和移植物的形态学变化。

Simvastatin may exert its function to prevent allograftangiopathy by the mechanism of cholesterol-lowering, the increase expression ofeNOS and secretion of NO, direct anti-inflammatory effect, commissural inhibitionwith cyclosporine on MHC-II and CD4~+ T cells.

他汀类药物可能通过降低血胆固醇水平、促进eNOS 的表达并增加NO 的分泌、直接的抗炎作用、与环孢素联合抑制MHC-II 的表达和T 淋巴细胞的活化增殖等机制来对抗同种异体血管硬化的作用。

The fusion rate of allograft+rhBMP-2 in lumbar posterolateral intertransverse fusion exeperiment in rabbits was 100%, and superior to autologous iliac crest and sustitute it.

得出结论：同种冷冻干燥异体骨+rhBMP-2复合物在兔子腰椎后外侧融合实验中，优于自体髂骨，可以取代自体髂骨，其融合率为100％，消除了假关节的发生。

Results:pedunculated omentum majus-cirded trachea transplantation ensured an early re-establishment of blood circulation of transplanted trachea and maintenance of its normal animate states; allogeneoilc transplantation of trachea activated bodys immune mechanism. resulting in a chronic process of rejection and finally leading to progressive necrosis of transplanted trachea tissues: chondroregeneration occurred at every period under perichondrium after transplantation. and the degree of regeneration was related with favorable blood circulation; the emplogyment of a supporting tube in trachea,under the condition of favorable re-establishment of early blood circulation, could preventgranulation tissue hyperplasia and keep trachea cavity unobstructed.Postgraduate Ding XiaoQuan Directed by-Lin LeShen

结果 带蒂大网膜包绕移植段气管行腹腔移植，早期重建了移植段气管的血液循环，保证了移植气管的早期成活及维持其正常生机状态；同种异体气管移植所激发机体的免疫机制，引起一个慢性排斥反应过程，导致移植段气管组织进行性坏死；软骨再生存在于移植后各个时间段，其再生程度与良好的血液循环有关；气管内支撑管的应用可防止肉芽组织增生堵塞管腔，从而保证气管管腔通畅。

As results, TEM showed characteristic ultrastructure morphology of apoptosis cell with chromatin marginal condense, nucleus pycnosis, mitochondrion vacuoles and cell shrinkage in IR injury group and no apoptosis cells in control group. Increased TUNEL-positive cell and typical DNA laddering were found and cells apoptotic rate were approximately 20% by flow cytometry assay in experimental groups.

值得探讨。本试验通过流式细胞仪、生物电镜、TUNEL法、RT-PCR以及DNA电泳等试验方法研究了带血供同种异体骨移植过程的细胞凋亡现象，并检测了Fas、FasL、Caspase-1、Caspase-3、Bcl-2等凋亡相关基因在IR损伤时的表达及变化情况，初步探讨了骨移植时IR损伤中细胞凋亡的发生机制及其在IR损伤中的生物学意义，希望为今后更深入的研究打下一定基础。

The adipose derived stem cells of inbreds train of rat wereinduced to differentiate into schwann-like cells and the cells were injectedinto the acellular nerve allograff.The proliferation or adhersion of these cells on the graft were observed by inverted microscope or ScanningElectronic Microscope. Activity of cells were detected using MTT. 10mmnerve gap of inbreds train of rats in two group were bridged by tissue-engineered peripheral nerve or autogenous nerve, the effect wereappraised by naked eye, recovery rate of sciatic function index,nerve-electrophysiological, histology, Transmission ElectronMicroscopy and quantitative analysis of recovery rate of myelinated fiberpopulations,diameter of myelinated fiber and thickness of myelin sheat.

按照前述方法培养并诱导近交系大鼠脂肪干细胞向类Sehwann分化，将诱导分化的脂肪干细胞悬液注入已制备的去细胞同种异体神经支架管中，倒置显微镜观察细胞生长情况；扫描电镜下观察细胞在材料上附着情况；四唑盐比色试验测定细胞活性；取两组近交系大鼠，分别用组织工程化神经和自体神经桥接10mm神经缺损，通过大体观察、坐骨神经功能指数恢复率的测定、神经电生理的测定、组织学光镜观察、透射电镜观察、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率的测定等指标评价实验效果。

Construct PLXRN-IL-1Ra and PLXRN-IL-10 vector, lapine synoviocytes were first transduced in culture by retroviral infection. The genetically modified synovial cells were then transplanted by intra-articular injection into the knee joints, assay of joint lavages confirmed that the gene expression was not lost after 14 days of transfer. Knees receiving the hIL-1Ra had significantly reduced cartilage breakdown. Delivery of the hIL-10 was less effective, When both genes were used together, there was a greater inhibition of cartilage breakdown and a considerable reduction of cartilage matrix degradation.

构建PLXRN-IL-1Ra、PLXRN-IL-10逆转录病毒载体，体外感染同种异体原代滑膜细胞，接着将转染了外源基因的滑膜细胞行关节腔注射入创伤性骨关节炎兔膝关节，通过对关节滑液的ELISA分析证明外源基因的表达在基因转移后14天还很稳定，只接受hIL-1Ra基因治疗的膝关节软骨损坏明显减轻，接受hIL-10基因治疗的膝关节治疗也有一定效果，当两种基因同时导入时，有非常明显的抑制软骨破坏和软骨基质降解的作用。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！