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Objective: To explore the potential of reconstruction of cervical vertebrae with the compound humeral cortical ring allograft packed with red bone morrow and cancellous allogenic bone, and find sonic evidence for the clinical practice of allogenic composite implants on reconstruction of vertebral body.

目的:探讨同种异体皮质骨环、自体红骨髓及异体松质骨复合骨重建颈椎的能力,为临床应用提供理论依据。

Methods: the first experiment Immunetolerance in mice sciatic nerve allograft by intrathymic injection of allogene: 48 mice were divided into 4 groups randomly: Group A,Group B, Group C and Group D(nerve allograft under immunosurppressant).

研究技术路线:第一部分:小鼠胸腺内注射异基因抗原在同种异体坐骨神经移植免疫耐受中的作用 1、动物分组:48只Balb/c小鼠随机分为4组,A组;B组;C组;D组(异体神经移植加用免疫抑制剂组)。

ConclusionThe first experiment Immunetolerance in mice sciatic nerve allograft by intrathymic injection of allogene : the specific immune tolerance can be induced in the sciatic nerve allograft by intrathymus injection of MHC allogene.

研究结论:第一部分:小鼠胸腺内注射异基因抗原在同种异体坐骨神经移植免疫耐受中的作用胸腺内注射异基因MHC抗原可诱导异体坐骨神经移植的特异性免疫耐受。

The cells were cultured under the following serum microenvironment. The primary cells of autoserum group were cultured with autoserum, changing the medium with fetal bovine serum after passage. The primary cells of homogeneity foreign serum group were cultured with homogeneity foreign serum, changing the medium with fetal bovine serum after passage. The primary cells of fetal bovine serum group were cultured with fetal bovine serum, and cultured with fetal bovine serum after passage. The primary cells of Dulbecco's modified Eagle's medium group were cultured with serum-free DMEM, changing the medium with fetal bovine serum after passage.

分别以下列血清微环境培养:自身血清组:分离细胞后原代用含大鼠自身血清的培养基培养,传代后换用含胎牛血清的培养基培养;同种异体血清组:分离细胞后原代用含同种异体血清的培养基培养,以后用胎牛血清培养基培养;胎牛血清组:分离后用含胎牛血清培养基培养,以后一直用含胎牛血清培养基培养;DMEM组:分离细胞后原代用不含血清的DMEM培养基培养,传代后换用含胎牛血清的培养基培养。

Objective: To study the survival and distribution of allogenic mesenchymal stem cells after implantation into other rabbits, evaluate osteogenetic effectiveness of collagen-sponge mixed with allogenic BMscs of rabbit in repairing of radial shaft defects, and lay the foundation for further application.

目的:了解兔骨髓间充质干细胞同种异体移植的存活分布情况及同种异体兔BMses复合胶原海绵情况,观察其对兔桡骨1.5cm缺损的修复效果,为将来的进一步应用莫定实验基础。

Then 3 rabbits with 6 limbs of each group were killed in the 1st, 3, 5, 7, 10 and 13th week respectively, to compare the bone healing roentgenologically and histologically There was receptive reaction in fresh homogenous bone implant ati...

实验证明脱钙同种异体骨是一种较好的植入物。彼优于新鲜异体骨,与新鲜自体骨植入无显著差异。新骨及软骨出现在植入物中而不在邻近宿主骨处,表明新骨形成可能是骨诱导作用的结果。

Allogeneic nerve grafts,heterogenous nerve grafts and synthetic materials are all nerve duct that bridge grafting neurologic defeat,in which the chemical extraction heterogenous nerve and the biodegradable nerve graft duct are the ideal materials.

自体神经、同种异体神经和合成材料均可作为桥接神经缺损的神经导管,以化学萃取的同种异体神经及可降解的生物材料导管较为理想。

Drill of cross pin system was used to make defects vertically to cartilage face at condylus medialis femoris of left knee joint in rabbits.

用横穿钉钻头在兔左膝关节股骨内侧髁垂直于软骨面制造缺损,缺损深4 mm,直径4.45 mm,自体移植组取右膝关节内侧髁骨软骨柱植入到左侧膝关节相应缺损处,异体移植组将经过程序性深低温冷冻保存的同种异体骨软骨柱移植到相应缺损处。

With the establishment of animal models of aortic homograft trans-plantation,using immunohistochemical staining,the differ-ence of the expression of MHCⅠantigen and MHCⅡanti-gen was detected between fresh aortic homografts and cryop-reserved aortic homografts.

建立大鼠同种异体主动脉移植模型,采用免疫组织化学实验研究新鲜主动脉和低温保存的主动脉同种异体移植后MHCⅠ类抗原和MHCⅡ类抗原表达的区别。

The results also showed that moxibustion serum could significantly enhance the susceptibility of tumor cells to lysis by Cytotoxicity T Lymphocytes in TILs in vitro, promote target distribution, raise activity of T cell clones which shows specific lysis to allogeneic tumor cells.

艾灸血清能明显提高指数生长期TIL中CTL的杀伤活性,提高CTL的靶向分布,增强对同种异体肿瘤特异性杀伤的T细胞克隆的活性,表现对同种异体肿瘤细胞较强的杀伤活性,而对异种肿瘤细胞低杀伤活性或不杀伤。

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