同种基因的

At the beginning, HLA class I/peptide complex teramer was prepared, including the most frequent alleles in Chinese, i.e., HLA-A2 and HLA-A24. Then the aAPCs were assembled by coating a cell-sized bead with pMHC and anti-CD28 antibody, which were able to provide the dual signals required for T cell activation, and used as stimulator to generate pMHC-specific CTL by co-culture with allogeneic peripheral blood lymphocyte.

首先制备包括我国人群基因频率最高的HLA-A2，HLA-A24的HLA/抗原肽四聚体，在此基础上，制备能够为T细胞提供第一、第二活化信号的aAPC，其表面包被的HLA/抗原肽复合物容易控制，用于诱导同种个体的T细胞产生同种CTL。

ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子，在许多同种的真核生物有它的同源基因，这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关，而这个家族的蛋白质和DNA的结合能，各种ARID蛋白质的专一性尽相同，过大致上偏好於和AT-rich的序结合；我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因，分别是garid1和garid2，我们首先对於garid1做分析；将AU1标记接到gARID1转染形鞭毛虫，用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下，过其阳性染色和蛋白质表现有明显增加；EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合，经由突变序分析，也显示gARID1的结合序为AGATC和AATAAAATA，随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

We obtained a 1.5Kb DNA fragment of the succinate dehydrogenase flavoprotein subunit by PCR amplification and carried out the homolog analysis with those of other organisms.The result indicated a high homologh in this fragment among the five organisms.

通过PCR方法得到了琥珀酸脱氢酶黄素蛋白基因的一个1.5KB大小的DNA片段，并与其它生物的同种酶的基因序列进行了同源性分析，发现在所比较的五种不同生物之间这个区段具有很高的同源性。

Methods The skeletal muscle stem cells were enzymatically isolated with collagen and dispase, and then purified by different adhesion time from 6 female SD rats. The purified cells were transinfected with adenovirus carrying lac-Z gene, and injected into the bladder neck of 6 autologous and 4 allogeneic rats. Tissues of blader neck and ureter were obtained in sacrified rats after 5 and 15 days, histological examination and X-gal staining were performed.

采用混合酶消化及反复差速贴壁法分离、纯化培养SD大鼠骨骼肌干细胞，然后用携带lac-Z基因的腺病毒载体转染肌干细胞，待转染成功后将携带lac-Z基因的骨骼肌干细胞注射于自体和同种异体的膀胱颈及后尿道周围，分别于注射后5、15d，处死大鼠取出膀胱颈及后尿道，行组织学检查及X-gal染色。

The cDNA squence of antibiotic, an antibacterial peptide, a process for preparing the said cDNA and a process for recombining the antibacterial peptide are disclosed. The present frequency of amino acid codens in Attacin A cDNA is analyzed. The high-frequency coden is introdued to its protein sequence to configure its cDNA sequence. The target gene is synthesized by chemical synthesis method or polymerase chain reaction.

本发明公开了葛佬素的cDNA序列，葛佬素cDNA的制备方法以及重组抗菌肽葛佬素的制备方法；通过对同种已知蛋白质Attacin A cDNA中氨基酸密码子的出现频度进行分析，采用出现频度高的密码子带入葛佬素蛋白质序列中进行葛佬素的cDNA序列构建，用化学合成法及聚合酶链式反应进行目的基因的合成，另外，采用基因重组法制备抗菌肽葛佬素，并测定了其活性。

But all act as markers that may be associated with different alleles of the same gene.

然而所有这些都用以指示下一步的研究，因为这些联系可能与同种基因体的不同等位基因相联接。

Construct PLXRN-IL-1Ra and PLXRN-IL-10 vector, lapine synoviocytes were first transduced in culture by retroviral infection. The genetically modified synovial cells were then transplanted by intra-articular injection into the knee joints, assay of joint lavages confirmed that the gene expression was not lost after 14 days of transfer. Knees receiving the hIL-1Ra had significantly reduced cartilage breakdown. Delivery of the hIL-10 was less effective, When both genes were used together, there was a greater inhibition of cartilage breakdown and a considerable reduction of cartilage matrix degradation.

构建PLXRN-IL-1Ra、PLXRN-IL-10逆转录病毒载体，体外感染同种异体原代滑膜细胞，接着将转染了外源基因的滑膜细胞行关节腔注射入创伤性骨关节炎兔膝关节，通过对关节滑液的ELISA分析证明外源基因的表达在基因转移后14天还很稳定，只接受hIL-1Ra基因治疗的膝关节软骨损坏明显减轻，接受hIL-10基因治疗的膝关节治疗也有一定效果，当两种基因同时导入时，有非常明显的抑制软骨破坏和软骨基质降解的作用。

Optionally, hematopoietic stem cells, autologous, syngeneic, allogeneic or xenogeneic, are delivered to increase the speed of regeneration of the patient's immune system.

可选择地，输入自体的、同种同基因的、同种异基因的、或异种异基因的造血干细胞，来增加患者免疫系统重建的速度。

The cells may be autologous, syngeneic, allogeneic or xenogeneic, as tolerance to foreign cells is created in the patient during reactivation of the thymus.

因为患者在胸腺重新活化过程中产生外来细胞抗性，因而这些细胞可以是自体的、同种同基因的、同种异基因的、或异种异基因的。

On the other hand, individuals who metabolised caffeine quickly and consumed two to three cups of coffee a day had a 22% reduction in the risk of heart attack compared with those with the same genetic makeup who consumed just one cup or less each day.

另一方面，那些咖啡因代谢较快的人，如果他们每天喝2杯至3杯咖啡，那么他们就会比那些拥有同种基因构成，但每天只喝1杯或更少量咖啡的人，患心脏病的几率少22％。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。