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Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因,用PCR、测序鉴定缺失菌株,通过动物实验观察clpE基因缺失株毒力改变情况,同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力,最后用实时荧光定量PCR分析自溶素(major autolysin A,lytA)、表面黏附素A(pneumococcal surface adhesion A,psaA)、溶血素(pneumolysin,ply)、肺炎球菌表面蛋白A(pneumococcal surface protein A, pspA和神经氨酸酶(neuraminidase, nanA)的表达。

The research results showed that there were no big difference in time of beginning fertilization between autotetraploid and diploid rice.

研究结果表明,同源四倍体水稻和二倍体水稻在发生受精作用的时间上没有存在着很大的差异。

The research results showed that there were no big difference in the time of beginning fertilization between autotetraploid and diploid rice.

研究结果表明,同源四倍体水稻和二倍体水稻在发生受精作用的时间上不存在很大的差异。

The changes of endogenous hormones in adventitious root formation in softwood cuttings of autotetraploid grape Muscat Hamburg were studied.

以葡萄同源四倍体玫瑰香和其二倍体玫瑰香母株的嫩枝插条为试材,研究了扦插生根过程中(0~18d)生根部位皮层的内源激素含量变化。

The formation and development of embryo sac in the diploid and its autotetraploid of rice cultivar IR36 was examined using Laser Scanning Confocal Microscopy.

利用激光扫描共聚焦显微镜术对水稻品种IR36二倍体及其同源四倍体的胚囊形成和发育过程进行了系统的研究。

To establish a new technique for in vitro rapid propagation and conservation of germplasm of autotetraploid plant of Plalycodon grandiflorum, basal MS medium supplemented with different hormones was filtrated and optimized by using uniform design and orthogonal design.

为了建立优良同源四倍体桔梗的快速繁殖和离体保存技术,采用均匀设计和正交设计以添加不同激素的MS培养基为基本培养基进行筛选和优化。

It is suggested that autotetraploidy rices are better females in the distant hybridization due to the fact that they are weaker in sexually reproductive ability.

结果暗示由于同源四倍体水稻具有比较弱的有性生殖能力,在远缘杂交中它们是比较好的杂交母本。

With the development of molecular biology, an efficient bacteriophage λ based Escherichia coli homologous recombination system has been developed for genetic engineering.

伴随着分子生物学的发展,一种基于λ噬菌体Red重组酶的同源重组系统已应用于大肠杆菌基因工程研究。

There are 3 and 4 homologous bolA genes in rice and Arabidopsis thaliana respectively, and genes encoding proteins with a SufE motif and a BolA motif are existed in both rice and Arabidopsis.

水稻和拟南芥中各有3和4个相关的同源基因,其中各有一个基因编码的蛋白还多含一个SufE结构域。

Vars. botrytis suggests that either AP1 or CAL gene plays a important role in the curd formation.

对拟南芥菜cauliflower突变体的分析及对结球植物花椰菜同源基因的分析结果表明,CAL与AP1基因可能与花球形成有关。

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