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The pHybLex/Zeo-Idl plasmid and the cDNA library plasmid were sequentially transformed into the yeast swains and screened to obtain Leu2^+ and Leu2^+LacZ^+ clones, with the false positive clones excluded using positive and negative controls, and the plasmid of the true positive clone was sequenced and blasted for homological analysis.

方法PCR方法扩增Id1的编码序列并定向克隆入诱饵质粒pHybLex/Zeo,构建重组诱饵质粒pHybLex/Zeo-Id1,酶切鉴定后转化入酵母株EGY48/pSH18-34,检测重组诱饵质粒有无非特异激活报告基因Leu2、LacZ作用;扩增并提取成人肺组织文库质粒,将文库质粒及重组诱饵质粒转化入酵母细胞,依次筛选Leu2^+,Leu2^+LacZ^+克隆;设置阴性及阳性对照,重复筛选Leu2+LacZ+克隆并排除假阳性克隆,获取真阳性文库质粒,进行测序和同源性比对。

Surprisingly a high degree of nucleotide identity was found with Staph.

令人惊讶的高度同源性,发现与金黄色葡萄球菌。

The sequence analysis result indicated that ompH is of hign homology between different serotypes.

序列分析结果表明,ompH基因在不同血清型之间具有较高的同源性。

Sequence analysis of PmSERK1 revealed high levels of homology to other plant SERK gene.

序列分析发现PmSERK1与其它植物的SERK基因高度同源。

A pair of primers spanned the ORF of goat IL-18BP gene stemming from sheep poxvirus genome were designed.

在羊痘病毒基因组序列中也可能有编码IL-18BP的同源基因。

Zhongshan Wang bronze inscription has a large number of paronyms, phonograms, homophones and dialect words, these are valuable material for phonology study in ancient times.

中山王铜器铭文中有大量同源词、谐声词、通假字和方言词,是研究上古音有价值的材料。

Today, the drug can be obtained through a semi-synthetic process from its precursors, including 10-deacetylbaccatin III, baccatin III and so on, which can be separated from yew tree.

现在紫杉醇以及它的抗癌同源衍生物可以通过其前体进行化学半合成制得。

Objective To explore the application value of CDX2 in differential diagnosis of gastric signet-ring cell carcinoma and xanthelasma.

目的探讨尾型同源盒转录因子-2(CDX2)在胃印戒细胞癌和黄色斑鉴别诊断中的应用价值。

A small joint in the middle of a bird's wing, homologous with the thumb and bearing three or four quill-like feathers.

小翼羽鸟翅中部的小骨节,与大拇指同源,上有三四片羽毛

After pathogenic fungi identified,we found 26 of them can cause cotton leaf spot. The 26 pathogenetic strains were carried out morphological and molecular identification of the firm. The initial identification of the pathogens is through the morphological observation such as the determination of growth, colony morphology, conidia and conidial stalk.

通过对生长量的测定,菌落形态,分生孢子和分生孢子梗等形态学观察进行形态学鉴定;通过PCR扩增真菌核糖体基因转录间隔区序列并测序,并将序列在GenBank上进行同源性比对和构建系统系统发育树等方法进行分子鉴定,鉴定出这26株真菌分属链格孢属、镰孢属、黑孢属、刺盘孢属、匍柄霉属等5个属。

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推荐网络例句

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了,但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场,你等着挨绳子吧!

Several theories about reigniting and extinguishing of the arc have been refered.

本文综合考虑了几种电弧重燃和熄灭理论。