同源的

According to sequence cluster analysis of the the tested samples, it indicates that the gene distance of RKN-9, RKN-8, RKN-21, RKN-11, RKN-18 , RKN-20, RKN-17, RKN-22, RKN-23, RKN-24 and RKN-25 to Meloidogyne incognita and Meloidogyne arenaria was very close with homology of 100% and 99%, respectively.

根据25个根结线虫种群序列比对分析和聚类分析可知，河北廊坊的番茄根结线虫、安徽宿州的黄瓜和番茄根结线虫、安徽太和的桔梗根结线虫、安徽和县的番茄根结线虫、浙江杭州的黄瓜根结线虫、四川成都的番茄根结线虫、安徽泗县的黄瓜根结线虫和中国农业大学四个不同番茄品种上的根结线虫的序列之间同源性为100％；它们与花生根结线虫和南方根结线虫(M.incognita)的遗传距离最近，序列同源性分别达到100％和99％。

The cDNA document of ACC oxidase gene of Citrus aurantium L. was cloned. By using the program of BLAST on NCBI GenBank database, the sequence presented a very high match with the ACC oxidase genes from other plants. The base sequence was analysed by using biology programe of DNAStar 5.0. 278 amino acids were coded by the base sequence and the base sequence had the same conserve region of ACC oxidase gene of many kinds of other plants. The base sequences comparability was more than 72% compared with those of many kinds of other plants. The amino acid sequences comparability was more than 70% compared with those of a lot of other plants.

克隆了酸橙1-氨基环丙烷－1-羟酸氧化酶基因cDNA片段，将片段序列在NCBI网站上进行同源性搜索，显示的皆为不同植物的ACC氧化酶基因，因而认为所克隆的片段就是酸橙ACC氧化酶基因；并运用DNAStar5.0软件进行序列分析，推导的氨基酸序列为278个残基；具有所有植物ACC氧化酶基因共有的保守区域；与多种植物的ACC氧化酶基因的核苷酸和氨基酸序列的同源性都在72%和70%以上。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

In order to direct the construction of plant germplasms by elucidating the relatives among plants at the level of gene, CYP86MF gene analogues from 11 species of 6 genera in Cuciferae were respectively obtained by PCR strategy using gene specific primers designed from conserved regions of CYP86MF gene reported. Sequence comparisonindicated that the similarities among the genes at nucleotide level were over 80%, and the similarities at amino acid level remained above 70%. The differences between the genes at nucleotide and amino acid level between species were 1.0%~ 5.7% and 2.6%~ 7.3% respectively, while those between genera 5.6%~ 22.5% and 7.3%~ 31.2%, respectively. Phylogenetic analysis showed that Brassica was closely related to Raphanus, followed by Rorippa Scop, Arabidopsis Heynh, Capsella Medic orderly, most distantly related to Orychophrogmus.

为了从分子水平阐明十字花科植物间的亲缘进化关系，给植物种质资源的创建提供理论依据，试验根据课题组已报道的CYP86MF基因编码的氨基酸保守区域设计特异引物，运用PCR技术分别从十字花科6个属11个物种中分离克隆到了CYP86MF基因的同源序列，经比较分析，结果表明：这些同源序列的相似性达80%以上，所推导的氨基酸序列相似性达70%以上，且两者种间差异分别为1.0%~ 5.7%和2.6%~ 7.3%，属间差异分别是5.6%~ 22.5%和7.3%~ 31.2%；由氨基酸序列构建的分子系统树可知，在亲缘进化关系上芸薹属与萝卜属较近，其他依次为蔊菜属、拟南芥属、荠菜属，而与诸葛菜属最远。

This work might lead to develop many better methods or means of preventing and controlling dysenteric diarrhea.To elucidate the role of H-NS protein in bacterial physiology, the hns gene insertion and deletion mutants were constructed with RecA and Red recombination system. Then, growth curve and invasion ability of wild strain and deletion mutant were studied.

为了更深入的了解hns基因在细菌生命活动中所起的作用，我们首先利用RecA重组系统构建了痢疾杆菌2457T hns基因的插入突变体；随后，对基于Red重组系统的痢疾杆菌2457T缺失突变体的构建方法进行了摸索并成功地敲除了hns、hdeA、hdeB、yhiE和yhiF 5个基因，研究表明延长同源臂的长度可以明显提高同源重组的效率，缩短构建缺失突变体所需的时间。

A new gene structure is proposed: head+body+tail, which allows the program with necessary complexity and putting some learning mechanism into the search process.② A new homeotic gene structure is proposed, it not only can call for subroutines easily, but also can automatically perform programming.③ The concept of different homeotic gene, a multi-cellular structure is proposed. It can be used to describe the complex multi-level programs and to implement the complex subroutine calls.④ An estimation of distribution operator for guiding search is proposed. It fuses statistic learning mechanism into the search process to accelerate the convergent process and improve the quality of solutions.

提出了一种新的基因结构：头＋身＋尾，使计算机自动设计的程序具有必要的复杂性，又便于引入学习机制；②提出了一种新的同源基因结构，它不仅可实现子程序的调用，还具有很强的编程能力；③提出了异族同源基因的概念：一种多细胞结构，它能描述复杂的多层次程序结构，实现可重用程序的复杂调用；④提出了分布估计变异方法，将统计学习机制融入算法，既提高了算法的收敛速度，又提高了解的质量。

The results of respiratory mechanics, homodynamic mechanics,aterial blood gaS, and inflaInInatory factors [TNF- Q, IL-l fl, IL-6,IL-l0, and adrenomedulin] which detected in animal serumand bronchial alveolar lavage fluid obtained from tiP lObe,heart lobe, and diaPhragIn lobe of right lung seParate1y in the mongreldogs were measured before and after lung injury (including receivinglung protective ventilation).

本实验证明了 loS肺内／肺外不同源性 ARDS动物模型的差异性，而且AnDS的肺部病变存在不均一性和多形性的特点，对有关ARDS进行性低氧血症的发生机制提出了肺动静脉可能存在类似于右一左分流的肺内分流现象，以及因肺内分流而导致严重通气／血流比例失调的解释。发现小潮气十PEEP通气对肺内源性 ARDS疗效不佳，而俯卧位＋小潮气－I－ PEEP通气对肺内／肺外不同源性ARDS均有较好的疗效。。

There are 11 gene bindings disappear in the normal group, but appear in the model group, and then disappear in the treat group, that indicates the 11 gene bindings are correlated with the epilepsy and Caohuozhimutang. After searching in the Gene Bank, 7 bindings in the 11 gene bindings are homogenic with the 8q31 ribosomal protein gene, 15p12 paraneoplastic neuronal antigen gene, 4q22 diacylglycerol kinase iota gene, xq37 FMR2 protein and 11p11 roudabout homolog 1, and 4 bindings of which are novel gene.

经NCBI美国国家基因库检索，这11条基因中的7条已知基因分别与位于鼠染色体8q31位置的核糖体蛋白、15p12位置的周围肿瘤神经元的抗原(paraneoplastic neuronal antigen)、4p22位置的甘油二酯酶、xq37位置的FMR2蛋白（FMR2 protein）的基因以及以及位于果蝇染色体11p11位置的交叉同族体（roundabout homolog 1）有极高的同源性；有4条基因片段同源性较低，为功能未知的新基因，已经在GeneBank进行了注册，注册号分别CK325391、CK325392、CK325393、CK325394。

Results We obtained a lysogenic bacteriophage MZTP01with clear plaque and 1 mm diameter. Fragment D with 2362bp (Genebank No. AY639599) was obtained after the phage DNA hydrolyzed by HindⅢ/EcoRⅠ. Among fragment D, the pep gene with molecular weight of 47 kDa and length of 1101bp was cloned and expressed. Recombinant M15 (pQE30pep) was built and overexpressed in Escherichia coli with a 47kDa clear band. At the same place a clear band was observed by Western blot. Judging from the time course expression, we could conclude that PEP protein produced at 1 hour after induction and then increased gradually. PEP protein was mainly in the form of inclusion body in the recombinant and slowed the growth speed of host. Homologous comparison of PEP protein from phage MZTP01 with other PEPs from BLAST were that phage MZTP01 PEP protein had 100% homologe with that of Escherichia coli K12, and most of others took the similarity in the range between 37%~84%.

诱导获得的溶原性噬菌体MZTP01斑点清晰，直径约1mm，成斑时间12h；从噬菌体基因组DNA双酶切(HindⅢ/EcoRⅠ)片段中回收长度为2362bp的D片段(Genbank登录号： AY639599)，又从D片段中克隆了长度为1101bp、编码367aa、分子量为47kDa的pep基因，表达载体M15(pQE30pep)在大肠杆菌(Escherichia coli， E.coli)中表达获得了47kDa的清晰表达带，在1h 时开始产生蛋白并有逐步上升的趋势； Western blot 也在47kDa处得到一条清晰的条带；可溶性分析表明PEP蛋白在重组菌株中是以不可溶的包含体形式存在的，该蛋白的产生明显地抑制了宿主的生长速度；噬菌体PEP氨基酸序列之间的同源性比较表明，噬菌体MZTP01 PEP蛋白与来自E.coli K12噬菌体的PEP蛋白的同源性程度最大。

Compared the similarity and difference of distribution of homologous fragments on X and Y, we suggested that the sex chromosomes of spiny eel have evolved from a pair of homologous ancestral chromosomes, and during the evolution, a pericentric inversion might have occurred on the original Y chromosome, and subsequently terminal regional duplication might have followed, eventually resulting in the differentiation and formation of X and Y chromosomes, then the recombinants between sex chromosomes were repressed, so the sex chromosomes could be inherited steadily.

根据X、Y染色体上同源片段的分布的相似和相异，我们对刺鳅性染色体的分化历程进行了推测，即：刺鳅的性染色体起源于一对原始的同源染色体，通过原Y染色体上的一个明显的臂间倒位，以及染色体末端的异染色质成分的扩增，导致原始Y染色体与X染色体在染色体结构上的分化，从而抑制了X、Y性染色体之间的遗传物质的重组交换，为性染色体的稳定遗传奠定了基础。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！