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It was reported an cDNA encoding HMG protein was cloned from Pharbitis nil.

HMG蛋白质含有一非常保守的区域HMG-box,为确定裂叶牵牛中是否有其它含HMG-box的蛋白质以及与HMG1的关系,我们根据已知的大豆、裂叶牵牛HMG设计出引物,利用RT-PCR扩增技术、Southern、Northern杂交技术,从裂叶牵牛中克隆出编码HMG蛋白质的另一个cDNA-HMG2,它与HMG-1同源性很高(氨基酸序列同源性〉80%),并对HMG1/2与裂叶牵牛开花功能的关系进行了研究。

The results showed that the gene and amino acid sequence homology between seven species are over 44.3% and 47.90/o, respectively. In addition, conservation of three sequences: EQCGSQAGGALCP, CCSQFOWCGST and CQSQC in the 40 amino acids sequence at the beginning of the N-terminal chitinase mature protein is very high, The conservation of eight Cys at the sites of 3,12,17,18,24,31,36,40 is 100% Finally, the chitinase signal peptide gene from Phaseolus vu?

结果表明七种基因之间有44.3%以上的同源性,蛋白之间有47.9%以上的同源性,而且在成熟蛋白N端前40个氨基酸序列中分别位于1~13、17~27和36~40位的EQCGSQAGGALCP、CCSQFGWCGST和CQSQC序列区保守性很高,尤其是位于第3、12、17、18、24、31、36、40位的八个半胱氨酸的保守性达到100%。

The CDA gene shares high sequence similarity with that of fungi including Rhizopus oryzae (75%), CDA1(58%) and CDA2(56%) of Rhizopus circinans, Mucor rouxii(56%), Gongreonella bulteri(48%), Rhizopus stolonifer(39%), Phycomyces blakesleeanus(39%), CDA1(17%) and CDA2(16%) of Saccharomyces cerevisiae.

总状毛霉CDA基因与其它相近种米根霉、卷柄根霉的CDA1和CDA2、鲁氏毛霉、卵形孢球托霉、匍枝根霉、布拉克须霉、酿酒酵母的CDA1和CDA2的基因序列同源性分别为:75%、58%、56%、56%、48%、39%、39%、17%和16%;相应的氨基酸序列的同源性分别为:69.0%、57%、59%、55%、47%、30%、32%、18%、21%。

In a giving stage after pollination,p53 homologue showed high levels in the antipodal cells,integument,immature endosperm,ovary wall,tracheary elements,and aleurone layer,while c-myc homologue showed low levels in these tissues,only before pollination showed high expression in polar nucleus.

结果发现,在授粉后的一定阶段,在反足细胞、珠被、未成熟的胚乳、子房壁、导管组织和糊粉层中,p53同源基因表达强烈,c-myc同源基因的表达相反,在授粉后的这些组织中基本不表达,而在授粉前的中央细胞的极核中表达水平较高。

Generally, two hybridization events have happened in the generation of gynogenetic crucian carp: the first one occurred about 6, 000 years ago when maternal ancestor of C. a. gibelio hybridized with males of C. a. auratus (ancestor of C. a. colored variety) and a diploid hybrid was produced; the second one happened over 10, 000 years ago, when the diploid hybrid was backcrossed with one of its parental population, by which the current triploid gynogenetic crucian carp emerged.

初步推断出东北雌核生殖银鲫是通过鲫鱼种群间的两次杂交产生的,第一次杂交是母本的银鲫同源种群与父本的彩鲫同源种群杂交,形成二倍体杂种;第二次杂交是二倍体杂种与两个祖先鲫鱼种群之一回交,形成了今天的三倍体杂种银鲫。

Selecting the better amplified from Diacylglycerol acyltransferase, gene, Acyl- desaturase gene ,then cloning sequencing, afer that, through the NCBI and nucleotide sequence to match homology , the results show that: cloning by the cassava, castor-oil plant Jatropha curcas DGAT and SAD genes and gene fragments have the high homology with other known plant DGAT genes and SAD genes .

从中筛选出扩增效果较好的二酰基甘油酰基转移酶(Diacylglycerol acyltransferase, DGAT)基因,硬脂酰-酰基载体蛋白脱饱和酶(Acyl- desaturase, SAD)基因克隆测序,经测序,通过NCBI与已知的核苷酸序列进行同源性比对,结果表明:克隆所获得的木薯、蓖麻和麻疯树 DGAT基因和SAD基因的片段与其它已知植物的DGAT基因和SAD基因具有很高的同源性。

Selecting the better amplified from Diacylglycerol acyltransferase, gene, Acyl- desaturase gene ,then cloning sequencing, afer that, through the NCBI and nucleotide sequence to match homology , the results show that: cloning by the cassava, castor-oil plant Jatropha curcas DGAT and SAD genes and gene fragments have the high homology with other known plant DGAT genes and SAD genes .

从中筛选出扩增效果较好的二基甘油基转移(Diacylglycerol acyltransferase, DGAT)基因,硬脂-基载体蛋白脱饱和(Acyl- desaturase, SAD)基因克隆测序,经测序,通过NCBI与已知的核酸序列进行同源性比对,结果表明:克隆所获得的木薯、蓖麻和麻疯树 DGAT基因和SAD基因的片段与其它已知植物的DGAT基因和SAD基因具有很高的同源性。

576 sera samples of drug user were collected, and HCV antibodies were detected by ELISA for those samples.

山西吸毒者HCV 1b型分离株在其核心区的144个核苷酸序列中,与日本、河北、上海、湖南及邻省的同源性都在94%以上,与湖南株的同源性最好(97%);而推导的氨基酸序列与日本的差异最大(7%)。

These authors were impressed with the exceptionally well-differentiated system of double vocal cords (homologous to man's true and false cords) as well as with animal's delicate arytenoids cartilages (for the homologous structure and its function in phonation see Fig. 2. 15),which indicate an ability to control and adjust the tension of the cords during phonation.

这些作家对异常分化良好的双声带系统(与人类真的及人造的声带同源)及动物精美的杓状软骨;杓状软骨在发声时可以控制及调整声带的张力(同源结构及发声功能见图2.15 )有极深的印象。

Bioinformatics was used to seek the homologous gene of EXT1 in Strongylocentrotus purpuratus which was just discovered and had the correlation to human hereditary multiple exostoses. The sequence, exons, coding protein, physical and chemical characters of EXT1 gene were analyzed. The structure and function of its coding protein were predicted, and the phylogenetic tree for the homologous gene was constructed, which provided certain basis for the future research of human EXT1 gene.

利用生物信息学方法寻找人类遗传多发性外生性骨疣EXT1基因在紫色球海胆中的同源基因,对该基因的序列、外显子信息、编码蛋白及其理化性质进行分析,并预测其编码蛋白的结构与功能,构建其同源基因的系统进化树,旨在为进一步研究人体EXT1基因提供一定的依据。

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